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Protein

Formate-dependent phosphoribosylglycinamide formyltransferase

Gene

purT

Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.UniRule annotation

Catalytic activityi

Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Formate-dependent phosphoribosylglycinamide formyltransferase (purT)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei815'-phosphoribosylglycinamideUniRule annotation1
Binding sitei113ATPUniRule annotation1
Binding sitei154ATPUniRule annotation1
Binding sitei202ATPUniRule annotation1
Metal bindingi266MagnesiumUniRule annotation1
Metal bindingi278MagnesiumUniRule annotation1
Binding sitei2855'-phosphoribosylglycinamideUniRule annotation1
Binding sitei3535'-phosphoribosylglycinamideUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 164ATPUniRule annotation6
Nucleotide bindingi194 – 197ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationImported
Biological processPurine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMFER573064:G1GFN-370-MONOMER.
UniPathwayiUPA00074; UER00127.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate-dependent phosphoribosylglycinamide formyltransferaseUniRule annotation
Alternative name(s):
5'-phosphoribosylglycinamide transformylase 2UniRule annotation
Formate-dependent GAR transformylaseUniRule annotation (EC:2.1.2.-UniRule annotation)
GAR transformylase 2UniRule annotation
Short name:
GART 2UniRule annotation
Non-folate glycinamide ribonucleotide transformylaseUniRule annotation
Phosphoribosylglycinamide formyltransferase 2UniRule annotation
Gene namesi
Name:purTUniRule annotation
Ordered Locus Names:Mefer_0364Imported
OrganismiMethanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)Imported
Taxonomic identifieri573064 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000001495 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi573064.Mefer_0364.

Structurei

3D structure databases

ProteinModelPortaliC7P6L7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini118 – 307ATP-graspInterPro annotationAdd BLAST190

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 225'-phosphoribosylglycinamide bindingUniRule annotation2
Regioni360 – 3615'-phosphoribosylglycinamide bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01598. Archaea.
COG0027. LUCA.
HOGENOMiHOG000072820.
KOiK08289.
OMAiGMVTMIT.
OrthoDBiPOG093Z039L.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_01643. PurT. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR016185. PreATP-grasp_dom_sf.
IPR005862. PurT.
PfamiView protein in Pfam
PF02222. ATP-grasp. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01142. purT. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

C7P6L7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIGTPLLKG SVKFLLLGSG ELGKEVVIEA QRLGVECIAV DRYQNAPAMQ
60 70 80 90 100
VAHRSYVIDM KDYDALMAVI EREKPDYIVP EIEAINTDAL IDAEKMGYNV
110 120 130 140 150
VPTAEATKIT MNRELIRRLA AEKLGLRTAK YAFADSLDEL RDAVENIGLP
160 170 180 190 200
CVVKPIMSSS GKGQSIIRRE EDIEKAWITA KEGARGLGNR VIVEEFINFD
210 220 230 240 250
YEITLLTART AEGTKFCEPI GHIQIDGDYH ESWQPHNMSD ELKEQAQKIA
260 270 280 290 300
KKVTDALGGY GIFGVELFVK EDEVIFSEVS PRPHDTGMVT MITQEMSEFE
310 320 330 340 350
IHVRAILGLP VSTKLIHPGA SHVIKAEINK YAPKYYIEEA LKVPNTKLRL
360 370 380
FGKPNAKIGR RMGVALAYAD SVEKARELAE KCAHAVKIE
Length:389
Mass (Da):43,213
Last modified:October 13, 2009 - v1
Checksum:i1D2274F0D0136346
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA. Translation: ACV24199.1.
RefSeqiWP_015790939.1. NC_013156.1.

Genome annotation databases

EnsemblBacteriaiACV24199; ACV24199; Mefer_0364.
GeneIDi8365033.
KEGGimfe:Mefer_0364.

Similar proteinsi

Entry informationi

Entry nameiC7P6L7_METFA
AccessioniPrimary (citable) accession number: C7P6L7
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: March 28, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported