UniProtKB - C7P6L7 (C7P6L7_METFA)
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Protein
Formate-dependent phosphoribosylglycinamide formyltransferase
Gene
purT
Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)
Status
Functioni
Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.UniRule annotation
Catalytic activityi
Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Formate-dependent phosphoribosylglycinamide formyltransferase (purT)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 81 | 5'-phosphoribosylglycinamideUniRule annotation | 1 | |
Binding sitei | 113 | ATPUniRule annotation | 1 | |
Binding sitei | 154 | ATPUniRule annotation | 1 | |
Binding sitei | 202 | ATPUniRule annotation | 1 | |
Metal bindingi | 266 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 278 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 285 | 5'-phosphoribosylglycinamideUniRule annotation | 1 | |
Binding sitei | 353 | 5'-phosphoribosylglycinamideUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 159 – 164 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 194 – 197 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: InterPro
- phosphoribosylglycinamide formyltransferase 2 activity Source: UniProtKB-UniRule
- phosphoribosylglycinamide formyltransferase activity Source: InterPro
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | TransferaseUniRule annotationImported |
Biological process | Purine biosynthesisUniRule annotation |
Ligand | ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MFER573064:G1GFN-370-MONOMER. |
UniPathwayi | UPA00074; UER00127. |
Names & Taxonomyi
Protein namesi | Recommended name: Formate-dependent phosphoribosylglycinamide formyltransferaseUniRule annotationAlternative name(s): 5'-phosphoribosylglycinamide transformylase 2UniRule annotation Formate-dependent GAR transformylaseUniRule annotation (EC:2.1.2.-UniRule annotation) GAR transformylase 2UniRule annotation Short name: GART 2UniRule annotation Non-folate glycinamide ribonucleotide transformylaseUniRule annotation Phosphoribosylglycinamide formyltransferase 2UniRule annotation |
Gene namesi | Name:purTUniRule annotation Ordered Locus Names:Mefer_0364Imported |
Organismi | Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)Imported |
Taxonomic identifieri | 573064 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › |
Proteomesi |
|
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 573064.Mefer_0364. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 118 – 307 | ATP-graspInterPro annotationAdd BLAST | 190 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 22 | 5'-phosphoribosylglycinamide bindingUniRule annotation | 2 | |
Regioni | 360 – 361 | 5'-phosphoribosylglycinamide bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the PurK/PurT family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG01598. Archaea. COG0027. LUCA. |
HOGENOMi | HOG000072820. |
KOi | K08289. |
OMAi | GMVTMIT. |
OrthoDBi | POG093Z039L. |
Family and domain databases
Gene3Di | 3.30.1490.20. 1 hit. |
HAMAPi | MF_01643. PurT. 1 hit. |
InterProi | View protein in InterPro IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013815. ATP_grasp_subdomain_1. IPR016185. PreATP-grasp_dom_sf. IPR005862. PurT. |
Pfami | View protein in Pfam PF02222. ATP-grasp. 1 hit. |
SUPFAMi | SSF52440. SSF52440. 1 hit. |
TIGRFAMsi | TIGR01142. purT. 1 hit. |
PROSITEi | View protein in PROSITE PS50975. ATP_GRASP. 1 hit. |
i Sequence
Sequence statusi: Complete.
C7P6L7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIGTPLLKG SVKFLLLGSG ELGKEVVIEA QRLGVECIAV DRYQNAPAMQ
60 70 80 90 100
VAHRSYVIDM KDYDALMAVI EREKPDYIVP EIEAINTDAL IDAEKMGYNV
110 120 130 140 150
VPTAEATKIT MNRELIRRLA AEKLGLRTAK YAFADSLDEL RDAVENIGLP
160 170 180 190 200
CVVKPIMSSS GKGQSIIRRE EDIEKAWITA KEGARGLGNR VIVEEFINFD
210 220 230 240 250
YEITLLTART AEGTKFCEPI GHIQIDGDYH ESWQPHNMSD ELKEQAQKIA
260 270 280 290 300
KKVTDALGGY GIFGVELFVK EDEVIFSEVS PRPHDTGMVT MITQEMSEFE
310 320 330 340 350
IHVRAILGLP VSTKLIHPGA SHVIKAEINK YAPKYYIEEA LKVPNTKLRL
360 370 380
FGKPNAKIGR RMGVALAYAD SVEKARELAE KCAHAVKIE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001696 Genomic DNA. Translation: ACV24199.1. |
RefSeqi | WP_015790939.1. NC_013156.1. |
Genome annotation databases
EnsemblBacteriai | ACV24199; ACV24199; Mefer_0364. |
GeneIDi | 8365033. |
KEGGi | mfe:Mefer_0364. |
Similar proteinsi
Entry informationi
Entry namei | C7P6L7_METFA | |
Accessioni | C7P6L7Primary (citable) accession number: C7P6L7 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 13, 2009 |
Last sequence update: | October 13, 2009 | |
Last modified: | March 28, 2018 | |
This is version 59 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |