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Protein

5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase

Gene

purP

Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.UniRule annotation

Catalytic activityi

ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase (purP)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateUniRule annotation1
Binding sitei94SubstrateUniRule annotation1
Binding sitei230ATPUniRule annotation1
Binding sitei258SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processPurine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMFER573064:G1GFN-311-MONOMER.
UniPathwayiUPA00074; UER00134.

Names & Taxonomyi

Protein namesi
Recommended name:
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseUniRule annotation (EC:6.3.4.23UniRule annotation)
Alternative name(s):
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligaseUniRule annotation
Gene namesi
Name:purPUniRule annotation
Ordered Locus Names:Mefer_0308Imported
OrganismiMethanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)Imported
Taxonomic identifieri573064 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000001495 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi573064.Mefer_0308.

Structurei

3D structure databases

ProteinModelPortaliC7P6G2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 348ATP-graspInterPro annotationAdd BLAST233

Sequence similaritiesi

Belongs to the phosphohexose mutase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04346. Archaea.
COG1759. LUCA.
HOGENOMiHOG000228474.
KOiK06863.
OMAiKFPGARG.
OrthoDBiPOG093Z04BP.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_01163. IMP_biosynth_PurP. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR023656. IMP_biosynth_PurP.
IPR009720. IMP_biosynth_PurP_C.
IPR010672. IMP_biosynth_PurP_N.
IPR016185. PreATP-grasp_dom_sf.
PANTHERiPTHR38147. PTHR38147. 1 hit.
PfamiView protein in Pfam
PF06849. DUF1246. 1 hit.
PF06973. DUF1297. 1 hit.
PIRSFiPIRSF004602. ATPgrasp_PurP. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

C7P6G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKEEILEI FDKYNKDEIT IATLGSHTSL HILKGAKLEG FSTVCITMKG
60 70 80 90 100
RDVPYKRFKV ADKFIYVDNF SDIKNEEIQE KLRELNAIVV PHGSFIAYCG
110 120 130 140 150
LDNVENNFLV PMFGNRAILR WESERSLEGK LLREAGVRVP KKYESPEDID
160 170 180 190 200
GTVIVKFPGA RGGRGYFIAS STEEFYKKAE ELKNRGILSD EDIANAHIEE
210 220 230 240 250
YVVGTNFCIH YFYSPLKDEV ELLGMDKRYE SNIDGLVRIP AKDQLEMDIN
260 270 280 290 300
PSYVITGNIP VVIRESLLPQ VFDIGDKLVA KAKELVPPGM IGPFCLQTLC
310 320 330 340 350
NENLELVVFE MSARVDGGTN SFMNGSPYSF LYNAEPLSMG QRIAREIKMA
360
LQLDMIDKVI S
Length:361
Mass (Da):40,790
Last modified:October 13, 2009 - v1
Checksum:iE3C12520B2C3DFBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA. Translation: ACV24144.1.
RefSeqiWP_015790884.1. NC_013156.1.

Genome annotation databases

EnsemblBacteriaiACV24144; ACV24144; Mefer_0308.
GeneIDi8364975.
KEGGimfe:Mefer_0308.

Similar proteinsi

Entry informationi

Entry nameiC7P6G2_METFA
AccessioniPrimary (citable) accession number: C7P6G2
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported