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C7P221 (C7P221_HALMD) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036
Ordered Locus Names:Hmuk_1124
OrganismHalomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541) (Haloarcula mukohataei) [Complete proteome] [HAMAP]
Taxonomic identifier485914 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halomicrobium

Protein attributes

Sequence length924 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036 SAAS SAAS022429

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036 SAAS SAAS022429

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6151Zinc By similarity HAMAP MF_00036
Metal binding6191Zinc By similarity HAMAP MF_00036
Metal binding7181Zinc By similarity HAMAP MF_00036
Metal binding7221Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
C7P221 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 23C28DF12FD109F8

FASTA924102,271
        10         20         30         40         50         60 
MSDLADEYRL DYFEKEGFER LTCGTCGDHF WTRDASRETC GEPPCAEYGF IDDPGFEETL 

        70         80         90        100        110        120 
SLEEMRETFL SFFEERDHGR VDPYPVAANR WRDDVLLTQA SIYDFQPHVT TGQSPPPSNP 

       130        140        150        160        170        180 
LVVSQPCIRM QDIDNVGKTG RHTMAFEMGG HHAFNADEGA GYAYEGEVYW KEETVQYCVE 

       190        200        210        220        230        240 
LFEELGVDSS ELTLIEDVWV GGGNAGPCFE VIYQGLELAT LVFMQFEQDP DGDYEMKDGN 

       250        260        270        280        290        300 
SYSEMDRRVV DTGYGIERWT WMSQGTPTVY EAIYPEMIEF LRDNAGLDYT DEEDEIVFRA 

       310        320        330        340        350        360 
AKLAGHMDID EAEDVEAARD TMAEQLGVET ARLVELMEPL EDIYAIADHS RTLAYMLGDG 

       370        380        390        400        410        420 
IVPSNVGTGY LARMVLRRTK RLVDTVGVDA PLDELVDMQA DRLGYDNRDT IRDIVRTEVE 

       430        440        450        460        470        480 
KYRETLDRGG RKVRQLADDY AEKDEPIPVQ ELIELYDSHG IQPDMVAEIA AERGVDVDVP 

       490        500        510        520        530        540 
DNFYGLVADR HGGGQAFEED ATVPHEKRLD ELPETDRLYY EDQQRMEFEA VVLEVFDRED 

       550        560        570        580        590        600 
GDYDVVLDQT MFYPEGGGQP PDHGTISTDD VTGEVTDVQV HGGVIVHTCD EDPGTGEFVR 

       610        620        630        640        650        660 
GQVDATRRRR LMRHHTATHV VIHSARQVLG EHVRQAGAQK GTDSSRIDIR HYESISREQV 

       670        680        690        700        710        720 
KEIEHRANEI VMDNAAVTQE WPDRHEAEKA HGFDLYQGGI PTGEQIRLIH VADDVQACGG 

       730        740        750        760        770        780 
THVGRTGDIG AIKLLNTERI QDGVIRLTFA AGDAAIDATH EIEDALYETA ELYDVATEDV 

       790        800        810        820        830        840 
PDTAARFFDE WKARGKEIED LKEQLAEARA SGGGDSEEIE VADTTAVVQR IDADMDELRA 

       850        860        870        880        890        900 
QANAVVDQGS IAVLGSGASG AQFVVAVPDG VAVNAGEVVG ELAGRVGGGG GGPSDFAQGG 

       910        920 
GPDADKLDDA LADAPEILRQ VANA

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001688 Genomic DNA. Translation: ACV47250.1.
RefSeqYP_003176957.1. NC_013202.1.

3D structure databases

ProteinModelPortalC7P221.
ModBaseSearch...

Protein-protein interaction databases

STRINGC7P221.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8410643.
GenomeReviewsGene locus Hmuk_1124 in contig CP001688_GR.
KEGGhmu:Hmuk_1124.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAMFTNSGM.
ProtClustDBPRK13902.

Family and domain databases

HAMAPMF_00036_A. Ala_tRNA_synth_A.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR022429. Ala-tRNA_synth_arc.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR03683. A-tRNA_syn_arch. 1 hit.
TIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7P221_HALMD
AccessionPrimary (citable) accession number: C7P221
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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