C7NZJ2 (C7NZJ2_HALMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase HAMAP MF_00435
EC=1.1.1.86 HAMAP MF_00435

Alternative name(s):
Acetohydroxy-acid isomeroreductase HAMAP MF_00435
Alpha-keto-beta-hydroxylacil reductoisomerase HAMAP MF_00435

Gene names

Name:	ilvC HAMAP MF_00435
Ordered Locus Names:	Hmuk_2654

Organism

Halomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541) (Haloarcula mukohataei) [Complete proteome] [HAMAP]

Taxonomic identifier

485914 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halomicrobium

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family. HAMAP MF_00435

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00435
Ligand	NADP HAMAP MF_00435
Molecular function	Isomerase EMBL ACV48760.1 Oxidoreductase HAMAP MF_00435 EMBL ACV48760.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	112	1	By similarity HAMAP MF_00435

Sequences

	Sequence		Length	Mass (Da)
	C7NZJ2 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 428EB9EAF33B19CC	FASTA	344	37,301
	10 20 30 40 50 60 MTDKLTTDVY YDEDVDASHI EDKTVAILGY GSQGHAQAQN LDDSGVDVVV GLRESSSSRT 70 80 90 100 110 120 EAEADGLRVE TPAAAASEAD IVVMLVPDTV QPDVYETIED GLDAGDTLQF AHGFNIHYGQ 130 140 150 160 170 180 IEPAADVDVT MVAPKSPGHL VRRTYKRDEG TPGLIAVEQD ATGDAKQEAL AYARAVGCAR 190 200 210 220 230 240 AGVIETTFQE EVETDLFGEQ AVLCGGVTDL VKAGFETLVD AGYAPEMAYF ECLNELKLIV 250 260 270 280 290 300 DLMYEGGHMG MWNSVSDTAE YGGLTRGESV IDREGMEKIL EEVQNGEFAR EWISENQANR 310 320 330 340 PAYKQYRSAE QNHQIERVGA DLRELFAWDG EDADADAAEA PADD

References

[1]

"Complete genome sequence of Halomicrobium mukohataei type strain (arg-2)." Tindall B.J., Schneider S., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Lucas S., Chen F., Tice H., Cheng J.F., Saunders E., Bruce D., Goodwin L., Pitluck S., Mikhailova N., Pati A., Mavromatis K., Ivanova N. , Chen A., Palaniappan K., Chain P., Land M., Hauser L., Chang Y.J., Jeffries C.D., Brettin T., Han C., Rohde M., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C., Detter J.C. Stand. Genomic Sci. 1:270-277(2009) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001688 Genomic DNA. Translation: ACV48760.1.
RefSeq	YP_003178467.1. NC_013202.1.
3D structure databases
ProteinModelPortal	C7NZJ2.
ModBase	Search...
Protein-protein interaction databases
STRING	C7NZJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8412204.
GenomeReviews	Gene locus Hmuk_2654 in contig CP001688_GR.
KEGG	hmu:Hmuk_2654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C7NZJ2_HALMD

Accession

Primary (citable) accession number: C7NZJ2

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	November 16, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)