ID   C7NVQ3_HALMD            Unreviewed;       449 AA.
AC   C7NVQ3;
DT   13-OCT-2009, integrated into UniProtKB/TrEMBL.
DT   13-OCT-2009, sequence version 1.
DT   27-MAR-2024, entry version 74.
DE   RecName: Full=Probable tRNA pseudouridine synthase D {ECO:0000256|HAMAP-Rule:MF_01082};
DE            EC=5.4.99.27 {ECO:0000256|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000256|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA pseudouridylate synthase D {ECO:0000256|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA-uridine isomerase D {ECO:0000256|HAMAP-Rule:MF_01082};
GN   Name=truD {ECO:0000256|HAMAP-Rule:MF_01082};
GN   OrderedLocusNames=Hmuk_0017 {ECO:0000313|EMBL:ACV46168.1};
OS   Halomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB
OS   13541) (Haloarcula mukohataei).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Halomicrobium.
OX   NCBI_TaxID=485914 {ECO:0000313|EMBL:ACV46168.1, ECO:0000313|Proteomes:UP000001746};
RN   [1] {ECO:0000313|EMBL:ACV46168.1, ECO:0000313|Proteomes:UP000001746}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541
RC   {ECO:0000313|Proteomes:UP000001746};
RX   PubMed=21304667; DOI=10.4056/sigs.42644;
RA   Tindall B.J., Schneider S., Lapidus A., Copeland A., Glavina Del Rio T.,
RA   Nolan M., Lucas S., Chen F., Tice H., Cheng J.F., Saunders E., Bruce D.,
RA   Goodwin L., Pitluck S., Mikhailova N., Pati A., Ivanova N., Mavrommatis K.,
RA   Chen A., Palaniappan K., Chain P., Land M., Hauser L., Chang Y.J.,
RA   Jeffries C.D., Brettin T., Han C., Rohde M., Goker M., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C.,
RA   Detter J.C.;
RT   "Complete genome sequence of Halomicrobium mukohataei type strain (arg-
RT   2).";
RL   Stand. Genomic Sci. 1:270-277(2009).
CC   -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC       uracil-13 in transfer RNAs. {ECO:0000256|HAMAP-Rule:MF_01082}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA;
CC         Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01082};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC       {ECO:0000256|ARBA:ARBA00007953, ECO:0000256|HAMAP-Rule:MF_01082}.
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DR   EMBL; CP001688; ACV46168.1; -; Genomic_DNA.
DR   RefSeq; WP_012807561.1; NC_013202.1.
DR   AlphaFoldDB; C7NVQ3; -.
DR   STRING; 485914.Hmuk_0017; -.
DR   GeneID; 8409513; -.
DR   KEGG; hmu:Hmuk_0017; -.
DR   eggNOG; arCOG04252; Archaea.
DR   HOGENOM; CLU_005281_4_1_2; -.
DR   OrthoDB; 1798at2157; -.
DR   Proteomes; UP000001746; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.1510.30; -; 1.
DR   Gene3D; 3.30.70.3160; -; 1.
DR   Gene3D; 3.30.2350.20; TruD, catalytic domain; 1.
DR   HAMAP; MF_01082; TruD; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001656; PsdUridine_synth_TruD.
DR   InterPro; IPR020119; PsdUridine_synth_TruD_CS.
DR   InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR   InterPro; IPR042214; TruD_catalytic.
DR   NCBIfam; TIGR00094; tRNA_TruD_broad; 1.
DR   PANTHER; PTHR13326:SF21; PSEUDOURIDYLATE SYNTHASE PUS7L; 1.
DR   PANTHER; PTHR13326; TRNA PSEUDOURIDINE SYNTHASE D; 1.
DR   Pfam; PF01142; TruD; 1.
DR   PIRSF; PIRSF037016; Pseudouridin_synth_euk_prd; 2.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS50984; TRUD; 1.
DR   PROSITE; PS01268; UPF0024; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01082};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001746};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW   Rule:MF_01082}.
FT   DOMAIN          176..408
FT                   /note="TRUD"
FT                   /evidence="ECO:0000259|PROSITE:PS50984"
FT   ACT_SITE        93
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01082"
SQ   SEQUENCE   449 AA;  48978 MW;  543E506E26DE3C6E CRC64;
     MRPAQPIEQA VGMRYYVSDG DGIGGRLRAS PEDFAVRELE AFEAEPADAD SGAYAHLVVR
     ATLHNWDTND FASALSDRLG ISRERVSWAG TKDKRAVTTQ LFSIADADAA DVPEIDGAEI
     DVVGRAGRPV LFGDLAGNAF EIIVREVDRP DGVDGITEQL REFAGSADAA DEPTVAVPNY
     FGQQRFGSKR PITHEVGLAI VRNDWEGAVM AYLGNPNERE PEATQAARAY VEETHDWEGA
     LERFPRALGY ERALCHSLAD ADDASPAAFR SALETLPTNL QTLFVNAAQS YLFNRVLSER
     LDRGLPFDRP VAGDVVCFAD SDAPDGLPLP DTDRTQRVTA GRVDTVERHC ERGRAFVTAP
     LVGTETELAE GEPGEIERAV LADAELEPTD FALPGEFDSS GTRRAVQVRS QIDVRRRDGD
     AVEFAFSLPK GSYATVLLRE YLKRDPAQL
//