Skip Header

Contribute Send feedback
Read comments (?) or add your own

C7NT23 (C7NT23_HALUD) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036
Ordered Locus Names:Huta_1929
OrganismHalorhabdus utahensis (strain DSM 12940 / JCM 11049 / AX-2) [Complete proteome] [HAMAP]
Taxonomic identifier519442 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halorhabdus

Protein attributes

Sequence length926 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036 SAAS SAAS022429

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036 SAAS SAAS022429

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6141Zinc By similarity HAMAP MF_00036
Metal binding6181Zinc By similarity HAMAP MF_00036
Metal binding7171Zinc By similarity HAMAP MF_00036
Metal binding7211Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
C7NT23 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: D763295F57A944F9

FASTA926102,257
        10         20         30         40         50         60 
MSDLEEEYRL EYFEEHGFHR KECMECGDHF WTLDGARETC GEPPCDEYSF IDDPGFDEAF 

        70         80         90        100        110        120 
ELGEMREQFL SFFEEHDHER IDPYPVAANR WREDVLLTQA SIYDFQPHVT SGASPPPANP 

       130        140        150        160        170        180 
LAVSQPCIRM QDIDNVGVTG RHTMAFEMMG HHAFNADEGT DYAYEGEVYW KDECVEYCMD 

       190        200        210        220        230        240 
LLAEVGADRE AVTLIEDPWV GGGNAGPAFE VIYEGAELAT LVFMQFERDP DGDFEMKDGN 

       250        260        270        280        290        300 
TYSRMDRRVI DTGYGLERWT WMSQGTPTVY EAVYPDMIDF LTDNAGIDHT ADEEEIVHKA 

       310        320        330        340        350        360 
AKLSGRMDID EAEDVEAARD DIAAEIGVAT DRLRELVEPL ESIYAIADHS RTLAYMLGDG 

       370        380        390        400        410        420 
IVPSNVGTGY LARMVLRRTK RLVDEVGVDA PLDELVDMQA ERLGYDNRDT IRDIVRTEVE 

       430        440        450        460        470        480 
KYRETLDRGH RRVEQLAEEY ADSGDPIPAD ELVELYDSHG MQPETVEQIA ADRGVAVETP 

       490        500        510        520        530        540 
EDFYSLVAER HEDAERAGST EESDTDERLA DLPETEKLYY DDQTRTEFEA VVLDVFERAD 

       550        560        570        580        590        600 
GYDVVLDQTM FYPEGGGQPP DQGTLSTDDE TVEVTDVQIQ DGVILHRTDA DPGTGDFVRG 

       610        620        630        640        650        660 
QVDGRRRRRL MAHHTATHIV VHAARQVLGD HIRQAGAQKG TDSARIDVRH YEPVSRAAVK 

       670        680        690        700        710        720 
EIERVANDIV TDNAQVTQEW PDRHEAEAEY GFDLYQGGIP AGEQIRLIHV EDDVQACGGT 

       730        740        750        760        770        780 
HVSRTGEVGA IKIRSTERIQ DGVVRLTFAA GEAAIEATQA TEDALYEAAE AFDVDPQDVP 

       790        800        810        820        830        840 
ETAERFFAEW KERGKEIEAL RAELAEVRAS GASEGEEVDL DGTTAVVQRV DGEMDELRAT 

       850        860        870        880        890        900 
ANAIADSGSV AVVGSAADGA QFVVAAPEGS GVDAGSVVSE LADRVGGGGG GPSDFAQGGG 

       910        920 
PDADALDAAL DAAPDVLRQV MRPSDR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001687 Genomic DNA. Translation: ACV12098.1.
RefSeqYP_003130831.1. NC_013158.1.

3D structure databases

ProteinModelPortalC7NT23.
ModBaseSearch...

Protein-protein interaction databases

STRINGC7NT23.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8384221.
GenomeReviewsGene locus Huta_1929 in contig CP001687_GR.
KEGGhut:Huta_1929.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAMFTNSGM.
ProtClustDBPRK13902.

Family and domain databases

HAMAPMF_00036_A. Ala_tRNA_synth_A.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR022429. Ala-tRNA_synth_arc.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR03683. A-tRNA_syn_arch. 1 hit.
TIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7NT23_HALUD
AccessionPrimary (citable) accession number: C7NT23
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: January 25, 2012
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info