C7NHF2 (C7NHF2_KYTSD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182
Ordered Locus Names:	Ksed_12770

Organism

Kytococcus sedentarius (strain ATCC 14392 / DSM 20547 / CCM 314 / 541) (Micrococcus sedentarius) [Complete proteome] [HAMAP]

Taxonomic identifier

478801 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Dermacoccaceae › Kytococcus › Kytococcus sedentarius

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS005794
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS005794
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS005794
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS005794 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	120 – 123	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	C7NHF2 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: C504F90096DD9BC3	FASTA	336	35,194
	10 20 30 40 50 60 MPLVRIVFAG TPEAALPSLE HLLDSARTAD GRDRHEVVGV LTRPDARVGR GRKLRPSPVK 70 80 90 100 110 120 ARALEHGLPV IESDRPWEDE PLASLRELAP DVGAIVAYGA LLPTSVLELP THGWVNLHFS 130 140 150 160 170 180 LLPAWRGAAP AQRALMAGDD LTGATTFVLT EGMDTGPVLG TLTEAIRPTD TAGDLLERLS 190 200 210 220 230 240 EAGAPLLTDS LHGLVSGALA PVPQPADGVS RAPKLTVDEV RLDFTQPAFA VVRRVAGASP 250 260 270 280 290 300 APGAWTTFRG DRLKVLQAAQ VAGAPSSTGA GTAGPDDPST PHLPPGRLLL HDGQVLVGTA 310 320 330 THPVRLQRVQ PPGKQAMEAE AWARGARFDD QEALGG

References

[1]

"Complete genome sequence of Kytococcus sedentarius type strain (541)." Sims D., Brettin T., Detter J.C., Han C., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Chen F., Lucas S., Tice H., Cheng J.F., Bruce D., Goodwin L., Pitluck S., Ovchinnikova G., Pati A., Ivanova N. , Mavrommatis K., Chen A., Palaniappan K., D'haeseleer P., Chain P., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Schneider S., Goker M., Pukall R., Kyrpides N.C., Klenk H.P. Stand. Genomic Sci. 1:12-20(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14392 / DSM 20547 / CCM 314 / 541.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001686 Genomic DNA. Translation: ACV06309.1.
RefSeq	YP_003149074.1. NC_013169.1.
3D structure databases
ProteinModelPortal	C7NHF2.
ModBase	Search...
Protein-protein interaction databases
STRING	478801.Ksed_12770.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACV06309; ACV06309; Ksed_12770.
GeneID	8372785.
KEGG	kse:Ksed_12770.
PATRIC	22192211. VBIKytSed14067_1214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	LPVIESD.
Enzyme and pathway databases
BioCyc	KSED478801:GI4L-1272-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C7NHF2_KYTSD

Accession

Primary (citable) accession number: C7NHF2

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)