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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.1 Publication

Enzyme regulationi

Inhibited by both galactose-6-phosphate and ATP.1 Publication

Kineticsi

  1. KM=0.54 mM for oNPbetagal6P (at pH 7.5)1 Publication

Vmax=50.94 µmol/min/mg enzyme with oNPbetagal6P as substrate1 Publication

pH dependencei

Optimum pH is 7.0-7.5.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donorBy similarity
Active sitei375 – 3751NucleophileBy similarity

GO - Molecular functioni

  1. 6-phospho-beta-galactosidase activity Source: UniProtKB
  2. galactosidase activity Source: UniProtKB

GO - Biological processi

  1. lactose catabolic process Source: UniProtKB
  2. lactose catabolic process via tagatose-6-phosphate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciLBUC523794:GHCR-615-MONOMER.
UniPathwayiUPA00542; UER00605.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidase (EC:3.2.1.85)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name:
PGALase
P-beta-Gal
Short name:
PBG
Gene namesi
Name:lacG
Ordered Locus Names:Lebu_0590
OrganismiLeptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249)
Taxonomic identifieri523794 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesLeptotrichiaceaeLeptotrichia
ProteomesiUP000001910 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4674676-phospho-beta-galactosidasePRO_0000398183Add
BLAST

Expressioni

Inductioni

Induced during growth on lactose or lactulose.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi523794.Lebu_0590.

Structurei

3D structure databases

ProteinModelPortaliC7N8L9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088632.
KOiK01220.
OMAiVKRFATF.
OrthoDBiEOG60658J.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C7N8L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKLPEDFI FGGATAAYQA EGAIKIDGKG PVAWDKFLEE NYWYTAEPAS
60 70 80 90 100
DFYHQYPVDL KLCEEFGING IRISIAWSRI FPNGYGEVNP KGVEFYHKLF
110 120 130 140 150
AECKKRKVEP FVTLHHFDTP EVLHSNGDFL NRENIEHFVN YAKFCFEEFS
160 170 180 190 200
EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY DFEKLFQSHH NMVLAHAKAV
210 220 230 240 250
NLFKKNGYHG EIGMVCALPT KYPYDPNNPK DVRAAELDDI IHNKFILDAT
260 270 280 290 300
FKGEYSKNTM EGVNHILQVN GGKLDLREED FEELKAAKDL NDFLGINYYM
310 320 330 340 350
SDWMAEYDGE TEIIHNATGN KGSSKYQIKG VGQRKANESI PRTDWDWIIY
360 370 380 390 400
PQGLYDQISR VKKDYPNYKK IYITENGLGY KDVFEDNTVY DDARIDYIRQ
410 420 430 440 450
HLEVISDAIK DGANVKGYFL WSLMDVFSWS NGYEKRYGLF YVDFETQKRY
460
PKKSAYWYKK VSETKEV
Length:467
Mass (Da):54,271
Last modified:October 13, 2009 - v1
Checksum:iD8642C3EEC575EC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001685 Genomic DNA. Translation: ACV38500.1.
RefSeqiYP_003163491.1. NC_013192.1.

Genome annotation databases

EnsemblBacteriaiACV38500; ACV38500; Lebu_0590.
KEGGilba:Lebu_0590.
PATRICi22400484. VBILepBuc70646_0602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001685 Genomic DNA. Translation: ACV38500.1.
RefSeqiYP_003163491.1. NC_013192.1.

3D structure databases

ProteinModelPortaliC7N8L9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi523794.Lebu_0590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV38500; ACV38500; Lebu_0590.
KEGGilba:Lebu_0590.
PATRICi22400484. VBILepBuc70646_0602.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088632.
KOiK01220.
OMAiVKRFATF.
OrthoDBiEOG60658J.

Enzyme and pathway databases

UniPathwayiUPA00542; UER00605.
BioCyciLBUC523794:GHCR-615-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249.
  2. "Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201."
    Thompson J.
    FEMS Microbiol. Lett. 214:183-188(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-33, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.

Entry informationi

Entry nameiLACG_LEPBD
AccessioniPrimary (citable) accession number: C7N8L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 13, 2009
Last modified: April 1, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.