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C7N8L9

- LACG_LEPBD

UniProt

C7N8L9 - LACG_LEPBD

Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (13 Oct 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.1 Publication

    Enzyme regulationi

    Inhibited by both galactose-6-phosphate and ATP.1 Publication

    Kineticsi

    1. KM=0.54 mM for oNPbetagal6P (at pH 7.5)1 Publication

    Vmax=50.94 µmol/min/mg enzyme with oNPbetagal6P as substrate1 Publication

    pH dependencei

    Optimum pH is 7.0-7.5.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei160 – 1601Proton donorBy similarity
    Active sitei375 – 3751NucleophileBy similarity

    GO - Molecular functioni

    1. 6-phospho-beta-galactosidase activity Source: UniProtKB
    2. galactosidase activity Source: UniProtKB

    GO - Biological processi

    1. lactose catabolic process Source: UniProtKB
    2. lactose catabolic process via tagatose-6-phosphate Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciLBUC523794:GHCR-615-MONOMER.
    UniPathwayiUPA00542; UER00605.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-galactosidase (EC:3.2.1.85)
    Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
    Short name:
    PGALase
    P-beta-Gal
    Short name:
    PBG
    Gene namesi
    Name:lacG
    Ordered Locus Names:Lebu_0590
    OrganismiLeptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249)
    Taxonomic identifieri523794 [NCBI]
    Taxonomic lineageiBacteriaFusobacteriaFusobacterialesLeptotrichiaceaeLeptotrichia
    ProteomesiUP000001910: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4674676-phospho-beta-galactosidasePRO_0000398183Add
    BLAST

    Expressioni

    Inductioni

    Induced during growth on lactose or lactulose.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi523794.Lebu_0590.

    Structurei

    3D structure databases

    ProteinModelPortaliC7N8L9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088632.
    KOiK01220.
    OMAiANESIPR.
    OrthoDBiEOG60658J.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    HAMAPiMF_01574. LacG.
    InterProiIPR005928. 6P-beta-galactosidase.
    IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR01233. lacG. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C7N8L9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKKLPEDFI FGGATAAYQA EGAIKIDGKG PVAWDKFLEE NYWYTAEPAS    50
    DFYHQYPVDL KLCEEFGING IRISIAWSRI FPNGYGEVNP KGVEFYHKLF 100
    AECKKRKVEP FVTLHHFDTP EVLHSNGDFL NRENIEHFVN YAKFCFEEFS 150
    EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY DFEKLFQSHH NMVLAHAKAV 200
    NLFKKNGYHG EIGMVCALPT KYPYDPNNPK DVRAAELDDI IHNKFILDAT 250
    FKGEYSKNTM EGVNHILQVN GGKLDLREED FEELKAAKDL NDFLGINYYM 300
    SDWMAEYDGE TEIIHNATGN KGSSKYQIKG VGQRKANESI PRTDWDWIIY 350
    PQGLYDQISR VKKDYPNYKK IYITENGLGY KDVFEDNTVY DDARIDYIRQ 400
    HLEVISDAIK DGANVKGYFL WSLMDVFSWS NGYEKRYGLF YVDFETQKRY 450
    PKKSAYWYKK VSETKEV 467
    Length:467
    Mass (Da):54,271
    Last modified:October 13, 2009 - v1
    Checksum:iD8642C3EEC575EC1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001685 Genomic DNA. Translation: ACV38500.1.
    RefSeqiYP_003163491.1. NC_013192.1.

    Genome annotation databases

    EnsemblBacteriaiACV38500; ACV38500; Lebu_0590.
    GeneIDi8407294.
    KEGGilba:Lebu_0590.
    PATRICi22400484. VBILepBuc70646_0602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001685 Genomic DNA. Translation: ACV38500.1 .
    RefSeqi YP_003163491.1. NC_013192.1.

    3D structure databases

    ProteinModelPortali C7N8L9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 523794.Lebu_0590.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACV38500 ; ACV38500 ; Lebu_0590 .
    GeneIDi 8407294.
    KEGGi lba:Lebu_0590.
    PATRICi 22400484. VBILepBuc70646_0602.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088632.
    KOi K01220.
    OMAi ANESIPR.
    OrthoDBi EOG60658J.

    Enzyme and pathway databases

    UniPathwayi UPA00542 ; UER00605 .
    BioCyci LBUC523794:GHCR-615-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    HAMAPi MF_01574. LacG.
    InterProi IPR005928. 6P-beta-galactosidase.
    IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR01233. lacG. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249.
    2. "Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201."
      Thompson J.
      FEMS Microbiol. Lett. 214:183-188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-33, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.

    Entry informationi

    Entry nameiLACG_LEPBD
    AccessioniPrimary (citable) accession number: C7N8L9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 13, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3