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C7N8L9 (LACG_LEPBD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-galactosidase

EC=3.2.1.85
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG
Gene names
Name:lacG
Ordered Locus Names:Lebu_0590
OrganismLeptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249) [Complete proteome] [HAMAP]
Taxonomic identifier523794 [NCBI]
Taxonomic lineageBacteriaFusobacteriaFusobacterialesLeptotrichiaceaeLeptotrichia

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. Ref.2

Enzyme regulation

Inhibited by both galactose-6-phosphate and ATP. Ref.2

Pathway

Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574

Induction

Induced during growth on lactose or lactulose. Ref.2

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.54 mM for oNPbetagal6P (at pH 7.5) Ref.2

Vmax=50.94 µmol/min/mg enzyme with oNPbetagal6P as substrate

pH dependence:

Optimum pH is 7.0-7.5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4674676-phospho-beta-galactosidase HAMAP-Rule MF_01574
PRO_0000398183

Sites

Active site1601Proton donor By similarity
Active site3751Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
C7N8L9 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: D8642C3EEC575EC1

FASTA46754,271
        10         20         30         40         50         60 
MSKKLPEDFI FGGATAAYQA EGAIKIDGKG PVAWDKFLEE NYWYTAEPAS DFYHQYPVDL 

        70         80         90        100        110        120 
KLCEEFGING IRISIAWSRI FPNGYGEVNP KGVEFYHKLF AECKKRKVEP FVTLHHFDTP 

       130        140        150        160        170        180 
EVLHSNGDFL NRENIEHFVN YAKFCFEEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY 

       190        200        210        220        230        240 
DFEKLFQSHH NMVLAHAKAV NLFKKNGYHG EIGMVCALPT KYPYDPNNPK DVRAAELDDI 

       250        260        270        280        290        300 
IHNKFILDAT FKGEYSKNTM EGVNHILQVN GGKLDLREED FEELKAAKDL NDFLGINYYM 

       310        320        330        340        350        360 
SDWMAEYDGE TEIIHNATGN KGSSKYQIKG VGQRKANESI PRTDWDWIIY PQGLYDQISR 

       370        380        390        400        410        420 
VKKDYPNYKK IYITENGLGY KDVFEDNTVY DDARIDYIRQ HLEVISDAIK DGANVKGYFL 

       430        440        450        460 
WSLMDVFSWS NGYEKRYGLF YVDFETQKRY PKKSAYWYKK VSETKEV 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Leptotrichia buccalis type strain (C-1013-b)."
Ivanova N., Gronow S., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Lucas S., Chen F., Tice H., Cheng J.F., Saunders E., Bruce D., Goodwin L., Brettin T., Detter J.C., Han C., Pitluck S., Mikhailova N. expand/collapse author list , Pati A., Mavrommatis K., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Chain P., Rohde C., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.
Stand. Genomic Sci. 1:126-132(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249.
[2]"Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201."
Thompson J.
FEMS Microbiol. Lett. 214:183-188(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 3-33, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001685 Genomic DNA. Translation: ACV38500.1.
RefSeqYP_003163491.1. NC_013192.1.

3D structure databases

ProteinModelPortalC7N8L9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING523794.Lebu_0590.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACV38500; ACV38500; Lebu_0590.
GeneID8407294.
KEGGlba:Lebu_0590.
PATRIC22400484. VBILepBuc70646_0602.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088632.
KOK01220.
OMAVKRFATF.
OrthoDBEOG60658J.
ProtClustDBPRK13511.

Enzyme and pathway databases

BioCycLBUC523794:GHCR-615-MONOMER.
UniPathwayUPA00542; UER00605.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
HAMAPMF_01574. LacG.
InterProIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01233. lacG. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLACG_LEPBD
AccessionPrimary (citable) accession number: C7N8L9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 13, 2009
Last modified: April 16, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries