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C7N8L9

- LACG_LEPBD

UniProt

C7N8L9 - LACG_LEPBD

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Protein
6-phospho-beta-galactosidase
Gene
lacG, Lebu_0590
Organism
Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.1 Publication

Enzyme regulationi

Inhibited by both galactose-6-phosphate and ATP.1 Publication

Kineticsi

  1. KM=0.54 mM for oNPbetagal6P (at pH 7.5)1 Publication

Vmax=50.94 µmol/min/mg enzyme with oNPbetagal6P as substrate

pH dependencei

Optimum pH is 7.0-7.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donor By similarity
Active sitei375 – 3751Nucleophile By similarity

GO - Molecular functioni

  1. 6-phospho-beta-galactosidase activity Source: UniProtKB
  2. galactosidase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. lactose catabolic process Source: UniProtKB
  2. lactose catabolic process via tagatose-6-phosphate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciLBUC523794:GHCR-615-MONOMER.
UniPathwayiUPA00542; UER00605.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidase (EC:3.2.1.85)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name:
PGALase
P-beta-Gal
Short name:
PBG
Gene namesi
Name:lacG
Ordered Locus Names:Lebu_0590
OrganismiLeptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249)
Taxonomic identifieri523794 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesLeptotrichiaceaeLeptotrichia
ProteomesiUP000001910: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4674676-phospho-beta-galactosidaseUniRule annotation
PRO_0000398183Add
BLAST

Expressioni

Inductioni

Induced during growth on lactose or lactulose.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi523794.Lebu_0590.

Structurei

3D structure databases

ProteinModelPortaliC7N8L9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088632.
KOiK01220.
OMAiANESIPR.
OrthoDBiEOG60658J.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C7N8L9-1 [UniParc]FASTAAdd to Basket

« Hide

MSKKLPEDFI FGGATAAYQA EGAIKIDGKG PVAWDKFLEE NYWYTAEPAS    50
DFYHQYPVDL KLCEEFGING IRISIAWSRI FPNGYGEVNP KGVEFYHKLF 100
AECKKRKVEP FVTLHHFDTP EVLHSNGDFL NRENIEHFVN YAKFCFEEFS 150
EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY DFEKLFQSHH NMVLAHAKAV 200
NLFKKNGYHG EIGMVCALPT KYPYDPNNPK DVRAAELDDI IHNKFILDAT 250
FKGEYSKNTM EGVNHILQVN GGKLDLREED FEELKAAKDL NDFLGINYYM 300
SDWMAEYDGE TEIIHNATGN KGSSKYQIKG VGQRKANESI PRTDWDWIIY 350
PQGLYDQISR VKKDYPNYKK IYITENGLGY KDVFEDNTVY DDARIDYIRQ 400
HLEVISDAIK DGANVKGYFL WSLMDVFSWS NGYEKRYGLF YVDFETQKRY 450
PKKSAYWYKK VSETKEV 467
Length:467
Mass (Da):54,271
Last modified:October 13, 2009 - v1
Checksum:iD8642C3EEC575EC1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001685 Genomic DNA. Translation: ACV38500.1.
RefSeqiYP_003163491.1. NC_013192.1.

Genome annotation databases

EnsemblBacteriaiACV38500; ACV38500; Lebu_0590.
GeneIDi8407294.
KEGGilba:Lebu_0590.
PATRICi22400484. VBILepBuc70646_0602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001685 Genomic DNA. Translation: ACV38500.1 .
RefSeqi YP_003163491.1. NC_013192.1.

3D structure databases

ProteinModelPortali C7N8L9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 523794.Lebu_0590.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACV38500 ; ACV38500 ; Lebu_0590 .
GeneIDi 8407294.
KEGGi lba:Lebu_0590.
PATRICi 22400484. VBILepBuc70646_0602.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088632.
KOi K01220.
OMAi ANESIPR.
OrthoDBi EOG60658J.

Enzyme and pathway databases

UniPathwayi UPA00542 ; UER00605 .
BioCyci LBUC523794:GHCR-615-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
HAMAPi MF_01574. LacG.
InterProi IPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR01233. lacG. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249.
  2. "Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201."
    Thompson J.
    FEMS Microbiol. Lett. 214:183-188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-33, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.

Entry informationi

Entry nameiLACG_LEPBD
AccessioniPrimary (citable) accession number: C7N8L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 13, 2009
Last modified: June 11, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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