C7N8L9 (LACG_LEPBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phospho-beta-galactosidase
EC=3.2.1.85

Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	Lebu_0590

Organism

Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249) [Complete proteome] [HAMAP]

Taxonomic identifier

523794 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Leptotrichiaceae › Leptotrichia › Leptotrichia buccalis

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. Ref.2
Enzyme regulation	Inhibited by both galactose-6-phosphate and ATP. Ref.2
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574
Induction	Induced during growth on lactose or lactulose. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Biophysicochemical properties	Kinetic parameters: KM=0.54 mM for oNPbetagal6P (at pH 7.5) Ref.2 Vmax=50.94 µmol/min/mg enzyme with oNPbetagal6P as substrate pH dependence: Optimum pH is 7.0-7.5.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	lactose catabolic process Inferred from direct assay Ref.2. Source: UniProtKB lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phospho-beta-galactosidase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	6-phospho-beta-galactosidase HAMAP-Rule MF_01574		PRO_0000398183
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	C7N8L9 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: D8642C3EEC575EC1	FASTA	467	54,271
	10 20 30 40 50 60 MSKKLPEDFI FGGATAAYQA EGAIKIDGKG PVAWDKFLEE NYWYTAEPAS DFYHQYPVDL 70 80 90 100 110 120 KLCEEFGING IRISIAWSRI FPNGYGEVNP KGVEFYHKLF AECKKRKVEP FVTLHHFDTP 130 140 150 160 170 180 EVLHSNGDFL NRENIEHFVN YAKFCFEEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY 190 200 210 220 230 240 DFEKLFQSHH NMVLAHAKAV NLFKKNGYHG EIGMVCALPT KYPYDPNNPK DVRAAELDDI 250 260 270 280 290 300 IHNKFILDAT FKGEYSKNTM EGVNHILQVN GGKLDLREED FEELKAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMAEYDGE TEIIHNATGN KGSSKYQIKG VGQRKANESI PRTDWDWIIY PQGLYDQISR 370 380 390 400 410 420 VKKDYPNYKK IYITENGLGY KDVFEDNTVY DDARIDYIRQ HLEVISDAIK DGANVKGYFL 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQKRY PKKSAYWYKK VSETKEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Leptotrichia buccalis type strain (C-1013-b)." Ivanova N., Gronow S., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Lucas S., Chen F., Tice H., Cheng J.F., Saunders E., Bruce D., Goodwin L., Brettin T., Detter J.C., Han C., Pitluck S., Mikhailova N. , Pati A., Mavrommatis K., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Chain P., Rohde C., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P. Stand. Genomic Sci. 1:126-132(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249.
[2]	"Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201." Thompson J. FEMS Microbiol. Lett. 214:183-188(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-33, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001685 Genomic DNA. Translation: ACV38500.1.
RefSeq	YP_003163491.1. NC_013192.1.
3D structure databases
ProteinModelPortal	C7N8L9.
ModBase	Search...
Protein-protein interaction databases
STRING	523794.Lebu_0590.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACV38500; ACV38500; Lebu_0590.
GeneID	8407294.
KEGG	lba:Lebu_0590.
PATRIC	22400484. VBILepBuc70646_0602.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2723.
HOGENOM	HOG000088632.
KO	K01220.
OMA	HARIVNA.
ProtClustDB	PRK13511.
Enzyme and pathway databases
BioCyc	LBUC523794:GHCR-615-MONOMER.
UniPathway	UPA00542; UER00605.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
HAMAP	MF_01574. LacG.
InterPro	IPR005928. 6P-beta-galactosidase. IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_LEPBD

Accession

Primary (citable) accession number: C7N8L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	October 13, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program