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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Saccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431ZincUniRule annotation
Binding sitei58 – 581Cysteinyl adenylateUniRule annotation
Binding sitei227 – 2271Cysteinyl adenylateUniRule annotation
Metal bindingi231 – 2311ZincUniRule annotation
Metal bindingi256 – 2561ZincUniRule annotation
Binding sitei283 – 2831Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSVIR471857:GHAV-2211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Svir_22240
OrganismiSaccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101)
Taxonomic identifieri471857 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharomonospora
Proteomesi
  • UP000000841 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400479Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi471857.Svir_22240.

Structurei

3D structure databases

ProteinModelPortaliC7MWX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 464Cysteinyl adenylate bindingUniRule annotation
Regioni81 – 833Cysteinyl adenylate bindingUniRule annotation
Regioni249 – 2513Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 5511"HIGH" regionUniRule annotationAdd
BLAST
Motifi187 – 1926"ERGGDP" regionUniRule annotation
Motifi289 – 2935"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

C7MWX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTWSSVAVP RVPGTSRPLR LYDTATGQIR PTAPGRVAKM YVCGITPYDA
60 70 80 90 100
THLGHAATYL AFDLVHRLWL DAGHEVHYVQ NVTDIDDPLL ERAERDSEDW
110 120 130 140 150
VVLGLRETAL FREDMEALRV LPPRDFVGAV ESIPEVVEMI EKLLASGAAY
160 170 180 190 200
RVDDPEYPDV YFDRSFTGRF GYESNYDDET MRAIFPERGG DPDRPGKRDP
210 220 230 240 250
LDALLWRVER PGEPAWDSSL GRGRPGWHIE CSAIALKHLG IGFDVQGGGS
260 270 280 290 300
DLVFPHHEFS AAHAEAMTGE HPFARHYVHA GMIGLDGEKM SKSKGNLVFV
310 320 330 340 350
SRLRADDVDP SAIRLALFAG HYRDDREWTD ELLKQANSRL ARWREAVSLP
360 370 380 390 400
SGPDAEATVD RLRDHLADDL DTPKALAAVD AWVDEALRHR SGTVGSESAP
410
ALVRAAVDSL LGVVL
Length:415
Mass (Da):46,005
Last modified:October 13, 2009 - v1
Checksum:iF68C433B0F43C1D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001683 Genomic DNA. Translation: ACU97232.1.
RefSeqiWP_015786545.1. NC_013159.1.

Genome annotation databases

EnsemblBacteriaiACU97232; ACU97232; Svir_22240.
KEGGisvi:Svir_22240.
PATRICi23394898. VBISacVir111818_2234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001683 Genomic DNA. Translation: ACU97232.1.
RefSeqiWP_015786545.1. NC_013159.1.

3D structure databases

ProteinModelPortaliC7MWX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi471857.Svir_22240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU97232; ACU97232; Svir_22240.
KEGGisvi:Svir_22240.
PATRICi23394898. VBISacVir111818_2234.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiPOG091H004K.

Enzyme and pathway databases

BioCyciSVIR471857:GHAV-2211-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_SACVD
AccessioniPrimary (citable) accession number: C7MWX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: October 13, 2009
Last modified: September 7, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.