C7MPF8 (C7MPF8_CRYCD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase HAMAP MF_00091
EC=4.4.1.21 HAMAP MF_00091

Alternative name(s):
AI-2 synthesis protein HAMAP MF_00091
Autoinducer-2 production protein LuxS HAMAP MF_00091

Gene names

Name:	luxS HAMAP MF_00091
Ordered Locus Names:	Ccur_11080

Organism

Cryptobacterium curtum (strain ATCC 700683 / DSM 15641 / 12-3) [Complete proteome] [HAMAP]

Taxonomic identifier

469378 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Coriobacteridae › Coriobacteriales › Coriobacterineae › Coriobacteriaceae › Cryptobacterium

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091 SAAS SAAS003815
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091 SAAS SAAS003815
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091 SAAS SAAS003815
Subunit structure	Homodimer By similarity. HAMAP MF_00091 SAAS SAAS003815
Sequence similarities	Belongs to the LuxS family. HAMAP MF_00091

Ontologies

Keywords
Biological process	Autoinducer synthesis HAMAP MF_00091 SAAS SAAS003815 Quorum sensing HAMAP MF_00091 SAAS SAAS003815
Ligand	Iron HAMAP MF_00091 SAAS SAAS003815 Metal-binding HAMAP MF_00091 SAAS SAAS003815
Molecular function	Lyase HAMAP MF_00091 SAAS SAAS003815
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	55	1	Iron By similarity HAMAP MF_00091
	Metal binding	59	1	Iron By similarity HAMAP MF_00091
	Metal binding	126	1	Iron By similarity HAMAP MF_00091

Sequences

	Sequence		Length	Mass (Da)
	C7MPF8 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 9340FA499F876784	FASTA	161	18,068
	10 20 30 40 50 60 MDKIASFTVD HLTLEPGVYV SREDVDVETG AVVTTFDIRM TAPNREPVMD TAAVHCLEHL 70 80 90 100 110 120 GATFMRNDAE WGSRIVYFGP MGCRTGFYLL VFGTVSSREI VGLLKRFCVF VRDFNGEVPG 130 140 150 160 ARPEECGNYH DSDLPQAQWW AQRFLDSTLE GIDADHLSYP V

References

[1]

"Complete genome sequence of Cryptobacterium curtum type strain (12-3)." Mavrommatis K., Pukall R., Rohde C., Chen F., Sims D., Brettin T., Kuske C., Detter J.C., Han C., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Lucas S., Tice H., Cheng J.F., Bruce D., Goodwin L. , Pitluck S., Ovchinnikova G., Pati A., Ivanova N., Chen A., Palaniappan K., Chain P., D'haeseleer P., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Rohde M., Klenk H.P., Kyrpides N.C. Stand. Genomic Sci. 1:93-100(2009) [PubMed: 21304644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700683 / DSM 15641 / 12-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001682 Genomic DNA. Translation: ACU94798.1.
RefSeq	YP_003151480.1. NC_013170.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C7MPF8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8375315.
GenomeReviews	Gene locus Ccur_11080 in contig CP001682_GR.
KEGG	ccu:Ccur_11080.
PATRIC	21526021. VBICryCur116861_1060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TTFDIRM.
ProtClustDB	PRK02260.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KO	K07173.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C7MPF8_CRYCD

Accession

Primary (citable) accession number: C7MPF8

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	December 14, 2011
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)