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C7MGF4 (C7MGF4_BRAFD) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Ordered Locus Names:Bfae_26120
OrganismBrachybacterium faecium (strain ATCC 43885 / DSM 4810 / NCIB 9860) [Complete proteome] [HAMAP]
Taxonomic identifier446465 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Dermabacteraceae › Brachybacterium

Protein attributes

Sequence length904 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
C7MGF4 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: D07EF91FBE3A52F4

FASTA90499,425
        10         20         30         40         50         60 
MTGGRHLVIV ESPAKARTIG RYLGEEYDVK ASVGHIRDIP VPRDLPAEMK KGPYGKFGVD 

        70         80         90        100        110        120 
VENGFDPYYI VSPDKKKTVA ELKKALKDAD TLYLATDEDR EGEAIAWHLL ETLKPKKSLP 

       130        140        150        160        170        180 
VKRMVFHEIT KDAIRAALEN TRDIDASLVD AQETRRILDR LVGFELSPVL WRKIKPSLSA 

       190        200        210        220        230        240 
GRVQSVATRL VVERERERMA FVAADYWDIT GTFAAEGGAD GTQFTAKIAT VDGARVATGS 

       250        260        270        280        290        300 
DFADDGTLKP RAKNAAVLTA ESTQTLVEVL GDAPATVTAL ESKPYTRRPA APFTTSSLQQ 

       310        320        330        340        350        360 
EASRKLRLGA RQAMRVAQSL YENGYITYMR TDSVTLSGEA IRASRAQAAE LYGSQFVPDK 

       370        380        390        400        410        420 
PRYYQNKSQA AQEAHEAIRP AGDHFRTPAE VAGQLSGDEF RLYELIWKRT IASQMADAKG 

       430        440        450        460        470        480 
TTSTVELLLE NEGRAATFRA AGTVITFRGF LAAYEEGRDA SRYKDDEEKG GDKRLPQMEE 

       490        500        510        520        530        540 
GQTLDTRALT PDGHTTTPPP RYTEASLVRA LEDRGIGRPS TYASTVATVQ DRGYVLRRGT 

       550        560        570        580        590        600 
ALVPSWTAFA VVRLLEEHFG PFIDYDFTAQ MEQQLDRMAQ GDLARSEYLK NFYLSDGTDG 

       610        620        630        640        650        660 
PIGLKPMVDD LGEIDARAIN SIEIGEGITL RVGRYGAYVE QFPRDEDGEL IEGADPRRAN 

       670        680        690        700        710        720 
VSDEVAPDEL TVEKAKEMLE RAKDDGRVLG TDPETGHEIV AKDGRYGPYV TEVLPEAQTA 

       730        740        750        760        770        780 
DGKKVPKSKQ PKPRTGSLLK SMDLSTVTLE DALKLLSLPR VVGTAADGTE ITAQNGRYGP 

       790        800        810        820        830        840 
YMKKGTDSRS LETEEQIFSI TLEEAEKIYA QPKTRGRAAA KPPLKELGTD PTSEKPIVIK 

       850        860        870        880        890        900 
DGRFGPYITD GTTNVSLRAT EKVEEITEKV AIEKLAEKRA KGPAKKKAPA KRTTTRKKST 


TTKK

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001643 Genomic DNA. Translation: ACU86387.1.
RefSeqYP_003155977.1. NC_013172.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGC7MGF4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8401157.
GenomeReviewsGene locus Bfae_26120 in contig CP001643_GR.
KEGGbfa:Bfae_26120.
PATRIC21176903. VBIBraFae133938_2575.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAKKVMPRL.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7MGF4_BRAFD
AccessionPrimary (citable) accession number: C7MGF4
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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