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C7M1S8 (C7M1S8_ACIFD) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Ordered Locus Names:Afer_1921
OrganismAcidimicrobium ferrooxidans (strain DSM 10331 / JCM 15462 / NBRC 103882 / ICP) [Complete proteome] [HAMAP]
Taxonomic identifier525909 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Acidimicrobidae › Acidimicrobiales › Acidimicrobineae › Acidimicrobiaceae › Acidimicrobium

Protein attributes

Sequence length850 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
C7M1S8 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 1B899AE1A0B7B62F

FASTA85094,456
        10         20         30         40         50         60 
MPKPLVIVES PTKARTIRRY LGDEWVVEAS VGHVRDLPRS AKEIPQAVKK EPWARLGVDT 

        70         80         90        100        110        120 
EHGFRPLYVI PPEKREQVKR LKALLADASE LFLATDEDRE GESIAWHLLE VLAPRVPVRR 

       130        140        150        160        170        180 
MVFHEITERA IREAVGATRG IDRGLVDAQE TRRVLDRLFG YEVSPVLWKK VMPRLSAGRV 

       190        200        210        220        230        240 
QSVACRLLVE RERERMAFRR ASYFDVVAEF SRAEAPAPFA ARLVALDEAR IATGRDFDAT 

       250        260        270        280        290        300 
GRVATGVVVL DGAHALAVAE AVEGRGGVVR ELTERPFTRR PDAPFMTSTL QQEASRKLRF 

       310        320        330        340        350        360 
SAQRTMAVAQ RLYEQGFITY MRTDSVQLAG EALAEARRLI AERFPGALPD APRQYRSRVK 

       370        380        390        400        410        420 
NAQEAHEAIR PAGTRWRSPD EVAREAPGDE ARLYELIWRR TLASQMTDAR GMSAVATIDV 

       430        440        450        460        470        480 
ALGPEVRIDG REIERATFVA RGRTLTDPGF LAVYPEQLSE DDEVDLPPLA AGVALTTTGA 

       490        500        510        520        530        540 
QPQGHETQPP ARYTEASLVR ALEERGIGRP STYAQIISTL VERSYAWRRS QALIPTFLGF 

       550        560        570        580        590        600 
AVVQLLERHF AELVDYSFTA RLEDDLDRIA IGEEEPTPYL AHFYWGNGAP GLHQLVTERL 

       610        620        630        640        650        660 
GEIDARDVNS IPIGQRADGT PVVVRVGRYG PFLQAGEETA SIPEDLPPDE LTLERAEELL 

       670        680        690        700        710        720 
ERGRLGDRVL GELDGQPVLL RTGRFGPYVQ LGEGDDRSLR RASLLPGQRV ETLSYEDAVA 

       730        740        750        760        770        780 
LLSLPRLVGV DPESQQEIWA HNGRFGPYLA KGRDTRSLDK PEQLFTIDLT EALARFREPK 

       790        800        810        820        830        840 
RRGRSASVLE LGTHPDTGAR VVVRSGRYGP YVSDGEVNAS LPRGVDPASL DLDTAVELLA 

       850 
ERRARLGSGS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001631 Genomic DNA. Translation: ACU54825.1.
RefSeqYP_003110498.1. NC_013124.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGC7M1S8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8324018.
GenomeReviewsGene locus Afer_1921 in contig CP001631_GR.
KEGGafo:Afer_1921.
PATRIC20643703. VBIAciFer34262_2027.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAKKVMPRL.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7M1S8_ACIFD
AccessionPrimary (citable) accession number: C7M1S8
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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