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Protein

Riboflavin biosynthesis protein RibBA

Gene

ribBA

Organism
Desulfomicrobium baculatum (strain DSM 4028 / VKM B-1378) (Desulfovibrio baculatus)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB), Riboflavin biosynthesis protein RibBA (ribBA)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.SAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Riboflavin biosynthesis protein RibBA (ribBA)
  2. Riboflavin biosynthesis protein RibD (Dbac_0062)
  3. Riboflavin biosynthesis protein RibD (Dbac_0062)
  4. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Magnesium or manganese 1UniRule annotation1
Metal bindingi28Magnesium or manganese 2UniRule annotation1
Binding sitei32D-ribulose 5-phosphateUniRule annotation1
Sitei126Essential for DHBP synthase activityUniRule annotation1
Binding sitei164D-ribulose 5-phosphateUniRule annotation1
Sitei164Essential for DHBP synthase activityUniRule annotation1
Metal bindingi257Zinc; catalyticUniRule annotation1
Metal bindingi268Zinc; catalyticUniRule annotation1
Metal bindingi270Zinc; catalyticUniRule annotation1
Binding sitei273GTPUniRule annotation1
Binding sitei317GTPUniRule annotation1
Active sitei329Proton acceptor; for GTP cyclohydrolase activityUniRule annotation1
Active sitei331Nucleophile; for GTP cyclohydrolase activityUniRule annotation1
Binding sitei352GTPUniRule annotation1
Binding sitei357GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi252 – 256GTPUniRule annotation5
Nucleotide bindingi295 – 297GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported, LyaseUniRule annotation, Multifunctional enzymeUniRule annotation
Biological processRiboflavin biosynthesisUniRule annotationSAAS annotation
LigandGTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciDBAC525897:G1GEX-61-MONOMER
UniPathwayiUPA00275; UER00399
UPA00275; UER00400

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Including the following 2 domains:
GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation)
Alternative name(s):
GTP cyclohydrolase IIUniRule annotation
3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation)
Short name:
DHBP synthaseUniRule annotation
Gene namesi
Name:ribBAUniRule annotation
Ordered Locus Names:Dbac_0060Imported
OrganismiDesulfomicrobium baculatum (strain DSM 4028 / VKM B-1378) (Desulfovibrio baculatus)Imported
Taxonomic identifieri525897 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfomicrobiaceaeDesulfomicrobium
Proteomesi
  • UP000002216 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi525897.Dbac_0060

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 372GTP_cyclohydro2InterPro annotationAdd BLAST165

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 201DHBP synthaseUniRule annotationAdd BLAST201
Regioni27 – 28D-ribulose 5-phosphate bindingUniRule annotation2
Regioni202 – 404GTP cyclohydrolase IIUniRule annotationAdd BLAST203

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C66 Bacteria
COG0108 LUCA
COG0807 LUCA
HOGENOMiHOG000115440
KOiK14652
OMAiGCTTGIS
OrthoDBiPOG091H008U

Family and domain databases

CDDicd00641 GTP_cyclohydro2, 1 hit
Gene3Di3.40.50.10990, 1 hit
HAMAPiMF_00179 RibA, 1 hit
MF_00180 RibB, 1 hit
MF_01283 RibBA, 1 hit
InterProiView protein in InterPro
IPR017945 DHBP_synth_RibB-like_a/b_dom
IPR000422 DHBP_synthase_RibB
IPR032677 GTP_cyclohydro_II
IPR000926 RibA
IPR036144 RibA-like_sf
IPR016299 Riboflavin_synth_RibBA
PfamiView protein in Pfam
PF00926 DHBP_synthase, 1 hit
PF00925 GTP_cyclohydro2, 1 hit
SUPFAMiSSF142695 SSF142695, 1 hit
SSF55821 SSF55821, 1 hit
TIGRFAMsiTIGR00505 ribA, 1 hit
TIGR00506 ribB, 1 hit

Sequencei

Sequence statusi: Complete.

C7LSH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKCSAEEAI KEIKAGKMII LVDDEDRENE GDLTIAAEMV TPEAINFMAK
60 70 80 90 100
YGRGLICLAL EPALVDKLEL PLMARRNTSK FGTNFTVSIE AKQGVTTGIS
110 120 130 140 150
AHDRALTIQT AVADQTTPED LATPGHIFPL RAKPGGVLVR AGQTEGSVDL
160 170 180 190 200
ARLAGLKGAA VICEIMNDDG SMSRMPDLRK FAEEHDMKIA TIADLIAYRS
210 220 230 240 250
RKDSLVRRVA EARMPTCYGE FTIVAYENDI DSHTHIALVK GEINEETPVL
260 270 280 290 300
VRVHSECLTG DVFGSMRCDC GSQLQRAMQM VNDEGAGVIL YMRQEGRGIG
310 320 330 340 350
LGNKIKAYHL QDEGRDTVEA NLELGFAPDL RDYGLGAQIL VDLGVKRMRL
360 370 380 390 400
LTNNPKKIIG LEGYGLKVEE RISIEIPACD DNKCYLHTKH SKLGHLLQFE

AENK
Length:404
Mass (Da):44,379
Last modified:October 13, 2009 - v1
Checksum:iD4CB39048F1F5266
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001629 Genomic DNA Translation: ACU88190.1
RefSeqiWP_012805275.1, NC_013173.1

Genome annotation databases

EnsemblBacteriaiACU88190; ACU88190; Dbac_0060
KEGGidba:Dbac_0060

Similar proteinsi

Entry informationi

Entry nameiC7LSH6_DESBD
AccessioniPrimary (citable) accession number: C7LSH6
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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