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C7LSH6

- C7LSH6_DESBD

UniProt

C7LSH6 - C7LSH6_DESBD

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Protein

Riboflavin biosynthesis protein RibBA

Gene

ribBA

Organism
Desulfomicrobium baculatum (strain DSM 4028 / VKM B-1378) (Desulfovibrio baculatus)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotationSAAS annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation
Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281Magnesium or manganese 1UniRule annotation
Metal bindingi28 – 281Magnesium or manganese 2UniRule annotation
Binding sitei32 – 321D-ribulose 5-phosphateUniRule annotation
Sitei126 – 1261Essential for DHBP synthase activityUniRule annotation
Binding sitei164 – 1641D-ribulose 5-phosphateUniRule annotation
Sitei164 – 1641Essential for DHBP synthase activityUniRule annotation
Metal bindingi257 – 2571Zinc; catalyticUniRule annotation
Metal bindingi268 – 2681Zinc; catalyticUniRule annotation
Metal bindingi270 – 2701Zinc; catalyticUniRule annotation
Binding sitei273 – 2731GTPUniRule annotation
Binding sitei317 – 3171GTPUniRule annotation
Active sitei329 – 3291Proton acceptor; for GTP cyclohydrolase activityUniRule annotation
Active sitei331 – 3311Nucleophile; for GTP cyclohydrolase activityUniRule annotation
Binding sitei352 – 3521GTPUniRule annotation
Binding sitei357 – 3571GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi252 – 2565GTPUniRule annotation
Nucleotide bindingi295 – 2973GTPUniRule annotation

GO - Molecular functioni

  1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. GTP cyclohydrolase II activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
  6. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. riboflavin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported, LyaseUniRule annotation

Keywords - Biological processi

Riboflavin biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

GTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciDBAC525897:GI50-62-MONOMER.
UniPathwayiUPA00275; UER00399.
UPA00275; UER00400.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Gene namesi
Name:ribBAUniRule annotation
Ordered Locus Names:Dbac_0060Imported
OrganismiDesulfomicrobium baculatum (strain DSM 4028 / VKM B-1378) (Desulfovibrio baculatus)Imported
Taxonomic identifieri525897 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfomicrobiaceaeDesulfomicrobium
ProteomesiUP000002216: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi525897.Dbac_0060.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 201201DHBP synthaseUniRule annotationAdd
BLAST
Regioni27 – 282D-ribulose 5-phosphate bindingUniRule annotation
Regioni202 – 404203GTP cyclohydrolase IIUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0108.
HOGENOMiHOG000115440.
KOiK14652.
OMAiGKGLICM.
OrthoDBiEOG679TK8.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFiPIRSF001259. RibA. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

C7LSH6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHKCSAEEAI KEIKAGKMII LVDDEDRENE GDLTIAAEMV TPEAINFMAK
60 70 80 90 100
YGRGLICLAL EPALVDKLEL PLMARRNTSK FGTNFTVSIE AKQGVTTGIS
110 120 130 140 150
AHDRALTIQT AVADQTTPED LATPGHIFPL RAKPGGVLVR AGQTEGSVDL
160 170 180 190 200
ARLAGLKGAA VICEIMNDDG SMSRMPDLRK FAEEHDMKIA TIADLIAYRS
210 220 230 240 250
RKDSLVRRVA EARMPTCYGE FTIVAYENDI DSHTHIALVK GEINEETPVL
260 270 280 290 300
VRVHSECLTG DVFGSMRCDC GSQLQRAMQM VNDEGAGVIL YMRQEGRGIG
310 320 330 340 350
LGNKIKAYHL QDEGRDTVEA NLELGFAPDL RDYGLGAQIL VDLGVKRMRL
360 370 380 390 400
LTNNPKKIIG LEGYGLKVEE RISIEIPACD DNKCYLHTKH SKLGHLLQFE

AENK
Length:404
Mass (Da):44,379
Last modified:October 13, 2009 - v1
Checksum:iD4CB39048F1F5266
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001629 Genomic DNA. Translation: ACU88190.1.
RefSeqiWP_012805275.1. NC_013173.1.
YP_003156606.1. NC_013173.1.

Genome annotation databases

EnsemblBacteriaiACU88190; ACU88190; Dbac_0060.
GeneIDi8375693.
KEGGidba:Dbac_0060.
PATRICi21702370. VBIDesBac69216_0058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001629 Genomic DNA. Translation: ACU88190.1 .
RefSeqi WP_012805275.1. NC_013173.1.
YP_003156606.1. NC_013173.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 525897.Dbac_0060.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACU88190 ; ACU88190 ; Dbac_0060 .
GeneIDi 8375693.
KEGGi dba:Dbac_0060.
PATRICi 21702370. VBIDesBac69216_0058.

Phylogenomic databases

eggNOGi COG0108.
HOGENOMi HOG000115440.
KOi K14652.
OMAi GKGLICM.
OrthoDBi EOG679TK8.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00399 .
UPA00275 ; UER00400 .
BioCyci DBAC525897:GI50-62-MONOMER.

Family and domain databases

Gene3Di 3.90.870.10. 1 hit.
HAMAPi MF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view ]
Pfami PF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001259. RibA. 1 hit.
SUPFAMi SSF55821. SSF55821. 1 hit.
TIGRFAMsi TIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 4028 / VKM B-1378Imported.

Entry informationi

Entry nameiC7LSH6_DESBD
AccessioniPrimary (citable) accession number: C7LSH6
Entry historyi
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation, Reference proteomeImported

External Data

Dasty 3