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C7LCL8 (C7LCL8_BRUMC) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL ACU48253.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:BMI_I1282 EMBL ACU48253.1
OrganismBrucella microti (strain CCM 4915) [Complete proteome] [HAMAP] EMBL ACU48253.1
Taxonomic identifier568815 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 346206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
C7LCL8 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 0851C55190A0EAF2

FASTA35337,550
        10         20         30         40         50         60 
MASGKMRIVV LFGGRSAEHD VSVLSATNVM NALDPAKYEA VPVFVTRAGQ WLLSRFVNGA 

        70         80         90        100        110        120 
LEKPSSGAEL CLVPGGCGRA IVVPDAGAPY EADKIDIIFP VLHGLHGEDG AVQGLAQVAR 

       130        140        150        160        170        180 
VPLAGCGIPG SANALDKDIA KRLFNEAGLS TAKSVTITRE EVPAFSALEQ ALGLPIFIKP 

       190        200        210        220        230        240 
ARQGSSVGVH KVVTEADYQA AMSDGFTYDD KLLAEEFIQA REVECGVLED EGGALFVSRA 

       250        260        270        280        290        300 
GEIVPAESHC FYSYDAKYID ADGTEIKVPA ELPEQVENEI RAIAAKAFRV LGCDSMARVD 

       310        320        330        340        350 
FFVTADRRIV LNEINTIPGF TDMSMYSKVM AVSGVSYPEI INRLVAHGLA RGS 

« Hide

References

[1]"Brucella microti: the genome sequence of an emerging pathogen."
Audic S., Lescot M., Claverie J.M., Scholz H.C.
BMC Genomics 10:352-352(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCM 4915 EMBL ACU48253.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001578 Genomic DNA. Translation: ACU48253.1.
RefSeqYP_003107202.1. NC_013119.1.

3D structure databases

ProteinModelPortalC7LCL8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING568815.BMI_I1282.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACU48253; ACU48253; BMI_I1282.
GeneID8320843.
KEGGbmr:BMI_I1282.
PATRIC17807578. VBIBruMic92249_2493.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBMIC568815:GJUE-1279-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7LCL8_BRUMC
AccessionPrimary (citable) accession number: C7LCL8
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: April 16, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)