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C7KGG6 (C7KGG6_ACEPA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase HAMAP-Rule MF_00206

EC=2.8.1.8 HAMAP-Rule MF_00206
Alternative name(s):
Lip-syn HAMAP-Rule MF_00206
Lipoate synthase HAMAP-Rule MF_00206
Lipoic acid synthase HAMAP-Rule MF_00206
Sulfur insertion protein LipA HAMAP-Rule MF_00206
Gene names
Name:lipA HAMAP-Rule MF_00206
ORF Names:APA26_12140 EMBL BAI11557.1
OrganismAcetobacter pasteurianus IFO 3283-26 [Complete proteome] EMBL BAI11557.1
Taxonomic identifier634456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_00206

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00206 SAAS SAAS003698.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_00206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding651Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding701Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding761Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding911Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding951Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206

Sequences

Sequence LengthMass (Da)Tools
C7KGG6 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 6558A0DE698EAFB5

FASTA32636,515
        10         20         30         40         50         60 
MSIRVVIDHR TGGSSPNKLR HPEKAHRPDN PIARKPDWIR VKAPNHPVYH ETRALMRSNK 

        70         80         90        100        110        120 
LVTVCEEAAC PNIGECWSQR HATMMIMGEI CTRACAFCNV TTGLPHALDA DEPTRVAEAV 

       130        140        150        160        170        180 
ARLGLRHVVI TSVDRDDLED GGAHHFARVI HAIRAAAPET TIEILTPDFL RKKNALEVVV 

       190        200        210        220        230        240 
EARPDVFNHN LETVPRLYPT IRPGARYYQS LRLLDRVKQL DASIFTKSGL MLGLGEEKME 

       250        260        270        280        290        300 
LAQVMDDLRV ADVDFITMGQ YLQPTVKHAP VARFVTPAEF EDYGTMARVK GFLQVSSSPL 

       310        320 
TRSSYHADAD FAALRDARNA RLKEDQ 

« Hide

References

[1]"Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus."
Azuma Y., Hosoyama A., Matsutani M., Furuya N., Horikawa H., Harada T., Hirakawa H., Kuhara S., Matsushita K., Fujita N., Shirai M.
Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: IFO 3283 EMBL BAI11557.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP011149 Genomic DNA. Translation: BAI11557.1.
RefSeqYP_005498857.1. NC_017146.1.

3D structure databases

ProteinModelPortalC7KGG6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAI11557; BAI11557; APA26_12140.
GeneID12087627.
KEGGapx:APA26_12140.
PATRIC36879179. VBIAcePas144932_1284.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03644.

Enzyme and pathway databases

BioCycAPAS634456:GL79-1218-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC7KGG6_ACEPA
AccessionPrimary (citable) accession number: C7KGG6
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: June 11, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)