C7GWZ2 (KEX1_YEAS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pheromone-processing carboxypeptidase KEX1
EC=3.4.16.6

Alternative name(s):
Carboxypeptidase D
Killer expression defective protein 1

Gene names

Name:	KEX1
ORF Names:	C1Q_05019

Organism

Saccharomyces cerevisiae (strain JAY291) (Baker's yeast) [Complete proteome]

Taxonomic identifier

574961 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from the precursors of K1, K2 and K28 killer toxins and a-factor (mating pheromone). Involved in the programmed cell death caused by defective N-glycosylation and contributes also to the active cell death program induced by acetic acid stress or during chronological aging. Promotes cell fusion by proteolytically processing substrates that act in parallel to PRM1 as an alternative fusion machine, as cell wall components, or both By similarity.
Catalytic activity	Preferential release of a C-terminal arginine or lysine residue.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 737	715	Pheromone-processing carboxypeptidase KEX1		PRO_0000411954
Regions
	Topological domain	23 – 624	602	Lumenal Potential
	Transmembrane	625 – 645	21	Helical; Potential
	Topological domain	646 – 737	92	Cytoplasmic Potential
	Compositional bias	526 – 531	6	Poly-Glu
	Compositional bias	545 – 561	17	Poly-Asp
	Compositional bias	567 – 586	20	Poly-Asp
	Compositional bias	605 – 615	11	Poly-Glu
	Compositional bias	711 – 719	9	Poly-Lys
Sites
	Active site	198	1	By similarity
	Active site	405	1	By similarity
	Active site	470	1	By similarity
Amino acid modifications
	Modified residue	668	1	Phosphoserine By similarity
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	459	1	N-linked (GlcNAc...) Potential
	Glycosylation	467	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	C7GWZ2 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 9379A1E2CBC66548	FASTA	737	83,179
	10 20 30 40 50 60 MFYNRWLGTW LAMSALIRIS VSLPSSEEYK VAYELLPGLS EVPDPSNIPQ MHAGHIPLRS 70 80 90 100 110 120 EDADEQDNSD LEYFFWKFTN NDSNGNVDRP LIIWLNGGPG CSSMDGALVE SGPFRVNSDG 130 140 150 160 170 180 KLYLNEGSWI SKGDLLFIDQ PTGTGFSVEQ NKDEGKIDKN KFDEDLEDVT KHFMDFLENY 190 200 210 220 230 240 FKIFPEDLTR KIILSGESYA GQYIPFFANA ILNHNKFSKI DGDTYDLKAL LIGNGWIDPN 250 260 270 280 290 300 TQSLSYLPFA MEKKLIDESN PNFKHLTNAH ENCQNLINSA STDEAAHFSY QECENILNLL 310 320 330 340 350 360 LSYTRESSQK GTADCLNMYN FNLKDSYPSC GMNWPKDVSF VSKFFSTPGV IDSLHLDSDK 370 380 390 400 410 420 IDHWKECTNS VGTKLSNPIS KPSIHLLPGL LESGIEIVLF NGDKDLICNN KGVLDTIDNL 430 440 450 460 470 480 KWGGIKGFSD DAVSFDWIHK SKSTDDSEEF SGYVKYDRNL TFVSVYNASH MVPFDKSLVS 490 500 510 520 530 540 RGIVDIYSND VMIIDNNGKN VMITTDDDSD QDATTESGDK PKENLEEEEQ EAQNEEGKEK 550 560 570 580 590 600 EGNKDKDGDD DNDNDDDDED DHNSEGDDDD DDDDDDDDDD DDDEDDNNEK QSNQGLEDSR 610 620 630 640 650 660 HKSSEYEQEE EEVEEFAEEI SMYKHKAVVV TIVTFLIVVL GVYAYDRRVR RKARHTILVD 670 680 690 700 710 720 PNNRQHDSPN KTVSWADDLE SGLGAEDDLE QDEQLEGGAP ISSTSNKAGS KLKTKKKKKY 730 TSLPNTEIDE SFEMTDF

References

[1]

"Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production." Argueso J.L., Carazzolle M.F., Mieczkowski P.A., Duarte F.M., Netto O.V.C., Missawa S.K., Galzerani F., Costa G.G.L., Vidal R.O., Noronha M.F., Dominska M., Andrietta M.G.S., Andrietta S.R., Cunha A.F., Gomes L.H., Tavares F.C.A., Alcarde A.R., Dietrich F.S. , McCusker J.H., Petes T.D., Pereira G.A.G. Genome Res. 19:2258-2270(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JAY291.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACFL01000397 Genomic DNA. Translation: EEU04662.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001563. Peptidase_S10. IPR018202. Peptidase_S10_AS. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. 1 hit. PS00131. CARBOXYPEPT_SER_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX1_YEAS2

Accession

Primary (citable) accession number: C7GWZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	October 13, 2009
Last modified:	October 3, 2012
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program