Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

C7GWZ2 (KEX1_YEAS2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pheromone-processing carboxypeptidase KEX1

EC=3.4.16.6
Alternative name(s):
Carboxypeptidase D
Killer expression defective protein 1
Gene names
Name:KEX1
ORF Names:C1Q_05019
OrganismSaccharomyces cerevisiae (strain JAY291) (Baker's yeast) [Complete proteome]
Taxonomic identifier574961 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from the precursors of K1, K2 and K28 killer toxins and a-factor (mating pheromone). Involved in the programmed cell death caused by defective N-glycosylation and contributes also to the active cell death program induced by acetic acid stress or during chronological aging. Promotes cell fusion by proteolytically processing substrates that act in parallel to PRM1 as an alternative fusion machine, as cell wall components, or both By similarity.

Catalytic activity

Preferential release of a C-terminal arginine or lysine residue.

Subcellular location

Golgi apparatustrans-Golgi network membrane; Single-pass type I membrane protein By similarity.

Sequence similarities

Belongs to the peptidase S10 family.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentGolgi apparatus
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionCarboxypeptidase
Hydrolase
Protease
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionserine-type carboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 737715Pheromone-processing carboxypeptidase KEX1
PRO_0000411954

Regions

Topological domain23 – 624602Lumenal Potential
Transmembrane625 – 64521Helical; Potential
Topological domain646 – 73792Cytoplasmic Potential
Compositional bias526 – 5316Poly-Glu
Compositional bias545 – 56117Poly-Asp
Compositional bias567 – 58620Poly-Asp
Compositional bias605 – 61511Poly-Glu
Compositional bias711 – 7199Poly-Lys

Sites

Active site1981 By similarity
Active site4051 By similarity
Active site4701 By similarity

Amino acid modifications

Modified residue6681Phosphoserine By similarity
Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Potential
Glycosylation4671N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
C7GWZ2 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 9379A1E2CBC66548

FASTA73783,179
        10         20         30         40         50         60 
MFYNRWLGTW LAMSALIRIS VSLPSSEEYK VAYELLPGLS EVPDPSNIPQ MHAGHIPLRS 

        70         80         90        100        110        120 
EDADEQDNSD LEYFFWKFTN NDSNGNVDRP LIIWLNGGPG CSSMDGALVE SGPFRVNSDG 

       130        140        150        160        170        180 
KLYLNEGSWI SKGDLLFIDQ PTGTGFSVEQ NKDEGKIDKN KFDEDLEDVT KHFMDFLENY 

       190        200        210        220        230        240 
FKIFPEDLTR KIILSGESYA GQYIPFFANA ILNHNKFSKI DGDTYDLKAL LIGNGWIDPN 

       250        260        270        280        290        300 
TQSLSYLPFA MEKKLIDESN PNFKHLTNAH ENCQNLINSA STDEAAHFSY QECENILNLL 

       310        320        330        340        350        360 
LSYTRESSQK GTADCLNMYN FNLKDSYPSC GMNWPKDVSF VSKFFSTPGV IDSLHLDSDK 

       370        380        390        400        410        420 
IDHWKECTNS VGTKLSNPIS KPSIHLLPGL LESGIEIVLF NGDKDLICNN KGVLDTIDNL 

       430        440        450        460        470        480 
KWGGIKGFSD DAVSFDWIHK SKSTDDSEEF SGYVKYDRNL TFVSVYNASH MVPFDKSLVS 

       490        500        510        520        530        540 
RGIVDIYSND VMIIDNNGKN VMITTDDDSD QDATTESGDK PKENLEEEEQ EAQNEEGKEK 

       550        560        570        580        590        600 
EGNKDKDGDD DNDNDDDDED DHNSEGDDDD DDDDDDDDDD DDDEDDNNEK QSNQGLEDSR 

       610        620        630        640        650        660 
HKSSEYEQEE EEVEEFAEEI SMYKHKAVVV TIVTFLIVVL GVYAYDRRVR RKARHTILVD 

       670        680        690        700        710        720 
PNNRQHDSPN KTVSWADDLE SGLGAEDDLE QDEQLEGGAP ISSTSNKAGS KLKTKKKKKY 

       730 
TSLPNTEIDE SFEMTDF 

« Hide

References

[1]"Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production."
Argueso J.L., Carazzolle M.F., Mieczkowski P.A., Duarte F.M., Netto O.V.C., Missawa S.K., Galzerani F., Costa G.G.L., Vidal R.O., Noronha M.F., Dominska M., Andrietta M.G.S., Andrietta S.R., Cunha A.F., Gomes L.H., Tavares F.C.A., Alcarde A.R., Dietrich F.S. expand/collapse author list , McCusker J.H., Petes T.D., Pereira G.A.G.
Genome Res. 19:2258-2270(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JAY291.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ACFL01000397 Genomic DNA. Translation: EEU04662.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS10.007.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

OrthoDBEOG7TJ3SJ.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERPTHR11802. PTHR11802. 1 hit.
PfamPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSPR00724. CRBOXYPTASEC.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKEX1_YEAS2
AccessionPrimary (citable) accession number: C7GWZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: October 13, 2009
Last modified: June 11, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries