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Protein

Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

C1Q_01026

Organism
Saccharomyces cerevisiae (strain JAY291) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathway:iUDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (C1Q_01026)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
ORF Names:C1Q_01026
OrganismiSaccharomyces cerevisiae (strain JAY291) (Baker's yeast)
Taxonomic identifieri574961 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000008073 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000393411Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC7GL41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 321320Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini393 – 532140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini565 – 710146SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

OrthoDBiEOG7034RG.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C7GL41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYCNF LIEKTRGEII DTLIEGLQAL EYKEYDSSGI SIQGDELKSL
60 70 80 90 100
NIYKQTGKIS SLKEEIDLYN LNKNLPFISH CGIAHTRRAT HGGLRRANCH
110 120 130 140 150
PHNSDPSNEF VVVHNGVITN FANLKALLVA KGYVFKSDTD TECIPKLYKH
160 170 180 190 200
IYDTSIELGY NLDFHVLTNL VLKELEGSYG LLCTSSHFPD EVVAARKGSP
210 220 230 240 250
LVIGVKGKTD MDVNFVEVEY LDQEEDYLKL NTQTKSSGNV LAAAPVKYNT
260 270 280 290 300
CLRKSPPLRS QYLRNSTTST FNHGSSTETP AENGLPRPME FYLSSDCASL
310 320 330 340 350
ARYVSKVVYL EDNDTAHIYD GELHIHCSKI GSEDFSFRTV QKLELELSKI
360 370 380 390 400
KKGPYDNFMQ KEIYEQCETT KNVMRGRVDA FTNRVVLGGL ENWLTELRRA
410 420 430 440 450
KRIIMIASKS SFHSCLAARP IFEELMEVPV NVELALDFVD RNCCIFRNDV
460 470 480 490 500
CIFVSRSGET TDTINALNYC IKKEAVTIGV VNCSGSSISR FTHCGVHTNT
510 520 530 540 550
GPEKGIATTK SYTSQYIALV MIALWMSEDL VSKIERRKEI IQALTIVPSQ
560 570 580 590 600
IKEVLELEPL IIELCDKKLK QHDTFLLLGR GYQFASALEG ASKMKEISYV
610 620 630 640 650
HSESILTDEL GHRVLAVTSD NPPIIAFATK DAFSPKIASC IDQIIERKGN
660 670 680 690 700
PIIICNKGHK IWEQDKQKGN VVTLEVPQTV DCLQGILNVI PLQLISYWLA
710 720
IKKDIGVDLP RDSAMSAPDI
Length:720
Mass (Da):80,561
Last modified:April 20, 2010 - v2
Checksum:iFCDCAB9E434094A6
GO

Sequence cautioni

The sequence EEU08477.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACFL01000033 Genomic DNA. Translation: EEU08477.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACFL01000033 Genomic DNA. Translation: EEU08477.1. Different initiation.

3D structure databases

ProteinModelPortaliC7GL41.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiEOG7034RG.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JAY291.

Entry informationi

Entry nameiYM084_YEAS2
AccessioniPrimary (citable) accession number: C7GL41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: July 22, 2015
This is version 28 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.