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C7G0B5

- PIF_PINFU

UniProt

C7G0B5 - PIF_PINFU

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Protein

Protein PIF

Gene
N/A
Organism
Pinctada fucata (Pearl oyster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the organic matrix for normal growth of the nacreous layer. The complex contributes to the initiation of aragonite crystallization as well as subsequenct stacking of aragonite tablets in the nacreous layer.
Pif80 binds to both aragonite and calcite crystals, with a higher specificity to aragonite crystals.
Pif97 contains a chitin-binding domain that allows for attachment of the entire complex to the chitin-containing organic framework.

GO - Molecular functioni

  1. chitin binding Source: InterPro

GO - Biological processi

  1. chitin metabolic process Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PIF
Cleaved into the following 2 chains:
Alternative name(s):
Aragonite-binding protein
OrganismiPinctada fucata (Pearl oyster)
Taxonomic identifieri50426 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaPterioidaPterioideaPteriidaePinctada

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 547525Protein Pif97PRO_5000503552Add
BLAST
Chaini548 – 1007460Protein Pif80PRO_5000503553Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi296 ↔ 309PROSITE-ProRule annotation
Disulfide bondi352 ↔ 364PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Component of conchiolin, the organic matrix of nacre. Expressed at extremely high levels in the dorsal region of the mantle, which region may be responsible for the nacreous layer formation, but only in trace amounts at the mantle edge, which region may be responsible for the prismatic layer formation.

Interactioni

Subunit structurei

Heterooligomer; disulfide-linked. Pif97, Pif80, N16 and other proteins form a complex.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 202174VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini255 – 372118Chitin-binding type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni544 – 5474Kex2-like proteinase cleavage site

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi367 – 981615Asp-richAdd
BLAST
Compositional biasi379 – 3857Poly-Asp
Compositional biasi388 – 3936Poly-Asp
Compositional biasi858 – 87215Poly-AspAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR002557. Chitin-bd_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00092. VWA. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 2 hits.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C7G0B5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVPSIRVVF LLTAVFCVGV KSDECKTADV VVNVDASDDV SDQDFDKLKR
60 70 80 90 100
AMLMMVRGLS IDDNQIRLGM VTYGSEVCDS IPLQGDRLDL ARTIRYMKKP
110 120 130 140 150
TGPSKPFKGM GEARRMFSSR GRYNVPHITM NLGGDIVDTE VKDLMDETDK
160 170 180 190 200
ARDEDIKVMA IGLGAKVDRD EIESIAYDRD QAYFMDDEDD LIRKVKEIPD
210 220 230 240 250
YLCKIIKAKK PKVSGGKKSK PAKKVDNGPA GKSPGFDALK QSDDKSDKAK
260 270 280 290 300
KVEVKELCDD AEWVDGVGYG SVPTRCEDFV MCQNVSGSLR KTLKSCPFGQ
310 320 330 340 350
YWSKRQTSCV LTEDEDCSDD LCKTMLLPSR EYDVSCRAYW KCEKGKSVAR
360 370 380 390 400
CCPSGMAYEP GKGCVLDLDC DEECPPKNDG DDDDDSSDED DDDEIEYNPN
410 420 430 440 450
CPLRPIKGHP EKFKQHTGDD NWEDFDCAPG TLFSARDCAC SILGTAKKDD
460 470 480 490 500
KNDDGGDAHK VCEPELYLPF CDDLHDYSGK ETHVENEGDA VIIENGKAYF
510 520 530 540 550
NGRAGLKIPR FSGVPYGKSV FIKMKYKEDE DDDKNKNDDD KKLRMKRDER
560 570 580 590 600
SRKDYLKAIL KRDDRKDKTD DTKGRRIIDR NDIIDDRRGR RKDDRKDGGR
610 620 630 640 650
DDGKDGRRDD RKDNRLDDIK DKNDEPMTLI SNGECDNFEL NDCFEKPSIA
660 670 680 690 700
ITTGKKSAGF SVTSSEKDEV DLEIDEDKKG YLWDKEDGPD RNGKDKDRNG
710 720 730 740 750
DRSDDRRGYY WKKKDKDDNG KDKDKKGDKS DDKKGYYWKK DKDDKNGKDK
760 770 780 790 800
DKRRDKSDDK KSLDDIVREI ERSKGNGKDD NKDDEDDDKK GWKTVSLKIS
810 820 830 840 850
NGHIRGRRDD REDKDVLDGD LKTTFSGFQI GQGASNKNFK GYMDEVYIYF
860 870 880 890 900
CDPGKEADYD DEDDDDDDDD SDENDKNDDK KDGKKTDDKD KKDRKDGRDD
910 920 930 940 950
RKDGRDDRKD RRDDRKDDRK GGKDDRKDDR KGGKDDRKDG RDVRDDRDRG
960 970 980 990 1000
DKYDKKDDKD NDRLSDKDDR KDVDDNDKDD DNEKLYKRAM KKCDYVNKNV

AKWLDKR
Length:1,007
Mass (Da):115,355
Last modified:October 13, 2009 - v1
Checksum:iA4C31FD25964F52B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB236929 mRNA. Translation: BAH97338.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

String of intrusion - Issue 112 of December 2009

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB236929 mRNA. Translation: BAH97338.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.140.10. 1 hit.
3.40.50.410. 1 hit.
InterProi IPR002557. Chitin-bd_dom.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF00092. VWA. 1 hit.
[Graphical view ]
SMARTi SM00494. ChtBD2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEi PS50940. CHIT_BIND_II. 2 hits.
PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An acidic matrix protein, Pif, is a key macromolecule for nacre formation."
    Suzuki M., Saruwatari K., Kogure T., Yamamoto Y., Nishimura T., Kato T., Nagasawa H.
    Science 325:1388-1390(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 548-564 AND 815-842, SUBUNIT, FUNCTION.
    Tissue: Mantle.
  2. "The molecular basis of nacre formation."
    Kroger N.
    Science 325:1351-1352(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPIF_PINFU
AccessioniPrimary (citable) accession number: C7G0B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 13, 2009
Last modified: October 29, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Pif80 and Pif97 are rich in Asp. This amino-acid appears to be common to biomineral-forming organisms.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3