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C7G0B5

- PIF_PINFU

UniProt

C7G0B5 - PIF_PINFU

Protein

Protein PIF

Gene
N/A
Organism
Pinctada fucata (Pearl oyster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 21 (01 Oct 2014)
      Sequence version 1 (13 Oct 2009)
      Previous versions | rss
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    Functioni

    Essential component of the organic matrix for normal growth of the nacreous layer. The complex contributes to the initiation of aragonite crystallization as well as subsequenct stacking of aragonite tablets in the nacreous layer.
    Pif80 binds to both aragonite and calcite crystals, with a higher specificity to aragonite crystals.
    Pif97 contains a chitin-binding domain that allows for attachment of the entire complex to the chitin-containing organic framework.

    GO - Molecular functioni

    1. chitin binding Source: InterPro

    GO - Biological processi

    1. chitin metabolic process Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein PIF
    Cleaved into the following 2 chains:
    Alternative name(s):
    Aragonite-binding protein
    OrganismiPinctada fucata (Pearl oyster)
    Taxonomic identifieri50426 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaPterioidaPterioideaPteriidaePinctada

    Subcellular locationi

    GO - Cellular componenti

    1. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 547525Protein Pif97PRO_5000503552Add
    BLAST
    Chaini548 – 1007460Protein Pif80PRO_5000503553Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi296 ↔ 309PROSITE-ProRule annotation
    Disulfide bondi352 ↔ 364PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Component of conchiolin, the organic matrix of nacre. Expressed at extremely high levels in the dorsal region of the mantle, which region may be responsible for the nacreous layer formation, but only in trace amounts at the mantle edge, which region may be responsible for the prismatic layer formation.

    Interactioni

    Subunit structurei

    Heterooligomer; disulfide-linked. Pif97, Pif80, N16 and other proteins form a complex.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 202174VWFAPROSITE-ProRule annotationAdd
    BLAST
    Domaini255 – 372118Chitin-binding type-2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni544 – 5474Kex2-like proteinase cleavage site

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi367 – 981615Asp-richAdd
    BLAST
    Compositional biasi379 – 3857Poly-Asp
    Compositional biasi388 – 3936Poly-Asp
    Compositional biasi858 – 87215Poly-AspAdd
    BLAST

    Sequence similaritiesi

    Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.170.140.10. 1 hit.
    3.40.50.410. 1 hit.
    InterProiIPR002557. Chitin-bd_dom.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF00092. VWA. 1 hit.
    [Graphical view]
    SMARTiSM00494. ChtBD2. 2 hits.
    SM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    SSF57625. SSF57625. 1 hit.
    PROSITEiPS50940. CHIT_BIND_II. 2 hits.
    PS50234. VWFA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C7G0B5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVPSIRVVF LLTAVFCVGV KSDECKTADV VVNVDASDDV SDQDFDKLKR     50
    AMLMMVRGLS IDDNQIRLGM VTYGSEVCDS IPLQGDRLDL ARTIRYMKKP 100
    TGPSKPFKGM GEARRMFSSR GRYNVPHITM NLGGDIVDTE VKDLMDETDK 150
    ARDEDIKVMA IGLGAKVDRD EIESIAYDRD QAYFMDDEDD LIRKVKEIPD 200
    YLCKIIKAKK PKVSGGKKSK PAKKVDNGPA GKSPGFDALK QSDDKSDKAK 250
    KVEVKELCDD AEWVDGVGYG SVPTRCEDFV MCQNVSGSLR KTLKSCPFGQ 300
    YWSKRQTSCV LTEDEDCSDD LCKTMLLPSR EYDVSCRAYW KCEKGKSVAR 350
    CCPSGMAYEP GKGCVLDLDC DEECPPKNDG DDDDDSSDED DDDEIEYNPN 400
    CPLRPIKGHP EKFKQHTGDD NWEDFDCAPG TLFSARDCAC SILGTAKKDD 450
    KNDDGGDAHK VCEPELYLPF CDDLHDYSGK ETHVENEGDA VIIENGKAYF 500
    NGRAGLKIPR FSGVPYGKSV FIKMKYKEDE DDDKNKNDDD KKLRMKRDER 550
    SRKDYLKAIL KRDDRKDKTD DTKGRRIIDR NDIIDDRRGR RKDDRKDGGR 600
    DDGKDGRRDD RKDNRLDDIK DKNDEPMTLI SNGECDNFEL NDCFEKPSIA 650
    ITTGKKSAGF SVTSSEKDEV DLEIDEDKKG YLWDKEDGPD RNGKDKDRNG 700
    DRSDDRRGYY WKKKDKDDNG KDKDKKGDKS DDKKGYYWKK DKDDKNGKDK 750
    DKRRDKSDDK KSLDDIVREI ERSKGNGKDD NKDDEDDDKK GWKTVSLKIS 800
    NGHIRGRRDD REDKDVLDGD LKTTFSGFQI GQGASNKNFK GYMDEVYIYF 850
    CDPGKEADYD DEDDDDDDDD SDENDKNDDK KDGKKTDDKD KKDRKDGRDD 900
    RKDGRDDRKD RRDDRKDDRK GGKDDRKDDR KGGKDDRKDG RDVRDDRDRG 950
    DKYDKKDDKD NDRLSDKDDR KDVDDNDKDD DNEKLYKRAM KKCDYVNKNV 1000
    AKWLDKR 1007
    Length:1,007
    Mass (Da):115,355
    Last modified:October 13, 2009 - v1
    Checksum:iA4C31FD25964F52B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB236929 mRNA. Translation: BAH97338.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    String of intrusion - Issue 112 of December 2009

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB236929 mRNA. Translation: BAH97338.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.140.10. 1 hit.
    3.40.50.410. 1 hit.
    InterProi IPR002557. Chitin-bd_dom.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF00092. VWA. 1 hit.
    [Graphical view ]
    SMARTi SM00494. ChtBD2. 2 hits.
    SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    SSF57625. SSF57625. 1 hit.
    PROSITEi PS50940. CHIT_BIND_II. 2 hits.
    PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An acidic matrix protein, Pif, is a key macromolecule for nacre formation."
      Suzuki M., Saruwatari K., Kogure T., Yamamoto Y., Nishimura T., Kato T., Nagasawa H.
      Science 325:1388-1390(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 548-564 AND 815-842, SUBUNIT, FUNCTION.
      Tissue: Mantle.
    2. "The molecular basis of nacre formation."
      Kroger N.
      Science 325:1351-1352(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPIF_PINFU
    AccessioniPrimary (citable) accession number: C7G0B5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: October 13, 2009
    Last modified: October 1, 2014
    This is version 21 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Pif80 and Pif97 are rich in Asp. This amino-acid appears to be common to biomineral-forming organisms.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3