Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

C7FF05

- PPO4_AGABI

UniProt

C7FF05 - PPO4_AGABI

Protein

Polyphenol oxidase 4

Gene

PPO4

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 20 (01 Oct 2014)
      Sequence version 1 (22 Sep 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Copper-containing oxidase that catalyzes both the o-hydroxylation of monophenols and the subsequent oxidation of the resulting o-diphenols into reactive o-quinones, which evolve spontaneously to produce intermediates, which associate in dark brown pigments. Involved in the initial step of melanin synthesis. Melanins constitute a mechanism of defense and resistance to stress such as UV radiations, free radicals, gamma rays, dehydratation and extreme temperatures, and contribute to the fungal cell-wall resistance against hydrolytic enzymes in avoiding cellular lysis. Fungal pigments are also involved in the formation and stability of spores By similarity.By similarity

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Copper 1; via tele nitrogenBy similarity
    Metal bindingi82 – 821Copper 1; via tele nitrogenBy similarity
    Metal bindingi91 – 911Copper 1; via tele nitrogenBy similarity
    Metal bindingi251 – 2511Copper 2; via tele nitrogenBy similarity
    Metal bindingi255 – 2551Copper 2; via tele nitrogenBy similarity
    Binding sitei255 – 2551SubstrateBy similarity
    Metal bindingi283 – 2831Copper 2; via tele nitrogenBy similarity
    Sitei379 – 3802CleavageCurated

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. monophenol monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyphenol oxidase 4 (EC:1.14.18.1)
    Short name:
    PPO4
    Short name:
    Phenolase 4
    Alternative name(s):
    Cresolase
    Tyrosinase 4
    Gene namesi
    Name:PPO4
    OrganismiAgaricus bisporus (White button mushroom)
    Taxonomic identifieri5341 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Polyphenol oxidase 4PRO_0000416866Add
    BLAST
    Propeptidei380 – 611232Removed in mature formCuratedPRO_0000416867Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki80 ↔ 822'-(S-cysteinyl)-histidine (Cys-His)By similarity

    Post-translational modificationi

    The C-ter is probably cleaved after Gly-379 since the mature active protein is smaller than the protein encoded by the gene.By similarity

    Keywords - PTMi

    Thioether bond

    Interactioni

    Subunit structurei

    Heterotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliC7FF05.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 1 hit.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C7FF05-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLLATVGPT GGVKNRLDIV DFVRDEKFFT LYVRALQAIQ DKDQADYSSF    50
    FQLSGIHGLP FTPWAKPKDT PTVPYESGYC THSQVLFPTW HRVYVSIYEQ 100
    VLQEAAKGIA KKFTVHKKEW VQAAEDLRQP YWDTGFALVP PDEIIKLEQV 150
    KITNYDGTKI TVRNPILRYS FHPIDPSFSG YPNFDTWRTT VRNPDADKKE 200
    NIPALIAKLD LEADSTREKT YNMLKFNANW EAFSNHGEFD DTHANSLEAV 250
    HDDIHGFVGR GAIRGHMTHA LFAAFDPIFW LHHSNVDRHL SLWQALYPGV 300
    WVTQGPEREG SMGFAPGTEL NKDSALEPFY ETEDKPWTSV PLTDTALLNY 350
    SYPDFDKVKG GTPDLVRDYI NDHIDRRYGI KKSEGGKNPA LDLLSDFKGV 400
    THDHNEDLKM FDWTIQASWK KFELDDSFAI IFYFAADGST NVTKENYIGS 450
    INIFRGTTPT NCANCRTQDN LVQEGFVHLD RFIARDLDTF DPQAVHRYLK 500
    EKKLSYKVVA DDHSVTLKSL RIRVQGRPLH LPPGVSFPRL DKNIPIVNFD 550
    DVLDLVTGVV NIGLTAVGAT AGVAIGVVGA TAGTAIGVAG AATDAVTNIA 600
    KGGLGALGRI F 611
    Length:611
    Mass (Da):68,318
    Last modified:September 22, 2009 - v1
    Checksum:iD9667D24204E614B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ354802 mRNA. Translation: ACU29458.1.
    GU936493 Genomic DNA. Translation: ADE67052.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GQ354802 mRNA. Translation: ACU29458.1 .
    GU936493 Genomic DNA. Translation: ADE67052.1 .

    3D structure databases

    ProteinModelPortali C7FF05.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 1 hit.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and expression of two new polyphenol oxidase cDNAs from Agaricus bisporus."
      Wu J., Chen H., Gao J., Liu X., Cheng W., Ma X.
      Biotechnol. Lett. 32:1439-1447(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning and characterization of two polyphenoloxidase genes from the mushrooms Agaricus bisporus."
      Li N.Y., Cai W.M., Liu C.Y., Ran F.L.
      Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: As2796.

    Entry informationi

    Entry nameiPPO4_AGABI
    AccessioniPrimary (citable) accession number: C7FF05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 20 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3