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C7FF05

- PPO4_AGABI

UniProt

C7FF05 - PPO4_AGABI

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Protein
Polyphenol oxidase 4
Gene
PPO4
Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Copper-containing oxidase that catalyzes both the o-hydroxylation of monophenols and the subsequent oxidation of the resulting o-diphenols into reactive o-quinones, which evolve spontaneously to produce intermediates, which associate in dark brown pigments. Involved in the initial step of melanin synthesis. Melanins constitute a mechanism of defense and resistance to stress such as UV radiations, free radicals, gamma rays, dehydratation and extreme temperatures, and contribute to the fungal cell-wall resistance against hydrolytic enzymes in avoiding cellular lysis. Fungal pigments are also involved in the formation and stability of spores By similarity.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Binds 2 copper ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Copper 1; via tele nitrogen By similarity
Metal bindingi82 – 821Copper 1; via tele nitrogen By similarity
Metal bindingi91 – 911Copper 1; via tele nitrogen By similarity
Metal bindingi251 – 2511Copper 2; via tele nitrogen By similarity
Metal bindingi255 – 2551Copper 2; via tele nitrogen By similarity
Binding sitei255 – 2551Substrate By similarity
Metal bindingi283 – 2831Copper 2; via tele nitrogen By similarity
Sitei379 – 3802Cleavage Inferred

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. melanin biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase 4 (EC:1.14.18.1)
Short name:
PPO4
Short name:
Phenolase 4
Alternative name(s):
Cresolase
Tyrosinase 4
Gene namesi
Name:PPO4
OrganismiAgaricus bisporus (White button mushroom)
Taxonomic identifieri5341 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Polyphenol oxidase 4
PRO_0000416866Add
BLAST
Propeptidei380 – 611232Removed in mature form Inferred
PRO_0000416867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki80 ↔ 822'-(S-cysteinyl)-histidine (Cys-His) By similarity

Post-translational modificationi

The C-ter is probably cleaved after Gly-379 since the mature active protein is smaller than the protein encoded by the gene By similarity.

Keywords - PTMi

Thioether bond

Interactioni

Subunit structurei

Heterotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliC7FF05.

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C7FF05-1 [UniParc]FASTAAdd to Basket

« Hide

MSLLATVGPT GGVKNRLDIV DFVRDEKFFT LYVRALQAIQ DKDQADYSSF    50
FQLSGIHGLP FTPWAKPKDT PTVPYESGYC THSQVLFPTW HRVYVSIYEQ 100
VLQEAAKGIA KKFTVHKKEW VQAAEDLRQP YWDTGFALVP PDEIIKLEQV 150
KITNYDGTKI TVRNPILRYS FHPIDPSFSG YPNFDTWRTT VRNPDADKKE 200
NIPALIAKLD LEADSTREKT YNMLKFNANW EAFSNHGEFD DTHANSLEAV 250
HDDIHGFVGR GAIRGHMTHA LFAAFDPIFW LHHSNVDRHL SLWQALYPGV 300
WVTQGPEREG SMGFAPGTEL NKDSALEPFY ETEDKPWTSV PLTDTALLNY 350
SYPDFDKVKG GTPDLVRDYI NDHIDRRYGI KKSEGGKNPA LDLLSDFKGV 400
THDHNEDLKM FDWTIQASWK KFELDDSFAI IFYFAADGST NVTKENYIGS 450
INIFRGTTPT NCANCRTQDN LVQEGFVHLD RFIARDLDTF DPQAVHRYLK 500
EKKLSYKVVA DDHSVTLKSL RIRVQGRPLH LPPGVSFPRL DKNIPIVNFD 550
DVLDLVTGVV NIGLTAVGAT AGVAIGVVGA TAGTAIGVAG AATDAVTNIA 600
KGGLGALGRI F 611
Length:611
Mass (Da):68,318
Last modified:September 22, 2009 - v1
Checksum:iD9667D24204E614B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GQ354802 mRNA. Translation: ACU29458.1.
GU936493 Genomic DNA. Translation: ADE67052.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GQ354802 mRNA. Translation: ACU29458.1 .
GU936493 Genomic DNA. Translation: ADE67052.1 .

3D structure databases

ProteinModelPortali C7FF05.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
InterProi IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
Pfami PF00264. Tyrosinase. 1 hit.
[Graphical view ]
PRINTSi PR00092. TYROSINASE.
SUPFAMi SSF48056. SSF48056. 1 hit.
PROSITEi PS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization and expression of two new polyphenol oxidase cDNAs from Agaricus bisporus."
    Wu J., Chen H., Gao J., Liu X., Cheng W., Ma X.
    Biotechnol. Lett. 32:1439-1447(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and characterization of two polyphenoloxidase genes from the mushrooms Agaricus bisporus."
    Li N.Y., Cai W.M., Liu C.Y., Ran F.L.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: As2796.

Entry informationi

Entry nameiPPO4_AGABI
AccessioniPrimary (citable) accession number: C7FF05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: September 22, 2009
Last modified: June 11, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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