ID   C7CE33_METED            Unreviewed;       326 AA.
AC   C7CE33;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   27-MAR-2024, entry version 76.
DE   SubName: Full=Cysteine synthase (O-acetylserine sulfhydrolase) {ECO:0000313|EMBL:CAX22737.1};
DE            EC=2.5.1.47 {ECO:0000313|EMBL:CAX22737.1};
GN   Name=cysK {ECO:0000313|EMBL:CAX22737.1};
GN   ORFNames=METD_I1120 {ECO:0000313|EMBL:CAX22737.1};
OS   Methylorubrum extorquens (strain DSM 6343 / CIP 106787 / DM4)
OS   (Methylobacterium extorquens).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=661410 {ECO:0000313|EMBL:CAX22737.1, ECO:0000313|Proteomes:UP000008070};
RN   [1] {ECO:0000313|Proteomes:UP000008070}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6343 / CIP 106787 / DM4
RC   {ECO:0000313|Proteomes:UP000008070};
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933,
CC         ECO:0000256|PIRSR:PIRSR605856-50};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis.
CC       {ECO:0000256|ARBA:ARBA00004895}.
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DR   EMBL; FP103042; CAX22737.1; -; Genomic_DNA.
DR   RefSeq; WP_003599935.1; NC_012988.1.
DR   AlphaFoldDB; C7CE33; -.
DR   GeneID; 72988335; -.
DR   KEGG; mdi:METDI1120; -.
DR   HOGENOM; CLU_021018_1_0_5; -.
DR   Proteomes; UP000008070; Chromosome.
DR   GO; GO:0004124; F:cysteine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006535; P:cysteine biosynthetic process from serine; IEA:InterPro.
DR   CDD; cd01561; CBS_like; 1.
DR   Gene3D; 3.40.50.1100; -; 2.
DR   InterPro; IPR005856; Cys_synth.
DR   InterPro; IPR005859; CysK.
DR   InterPro; IPR001926; TrpB-like_PALP.
DR   InterPro; IPR036052; TrpB-like_PALP_sf.
DR   NCBIfam; TIGR01139; cysK; 1.
DR   NCBIfam; TIGR01136; cysKM; 1.
DR   PANTHER; PTHR10314; CYSTATHIONINE BETA-SYNTHASE; 1.
DR   PANTHER; PTHR10314:SF253; CYSTEINE SYNTHASE 1; 1.
DR   Pfam; PF00291; PALP; 1.
DR   SUPFAM; SSF53686; Tryptophan synthase beta subunit-like PLP-dependent enzymes; 1.
PE   4: Predicted;
KW   Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW   ECO:0000256|PIRSR:PIRSR605856-50};
KW   Transferase {ECO:0000313|EMBL:CAX22737.1}.
FT   DOMAIN          22..310
FT                   /note="Tryptophan synthase beta chain-like PALP"
FT                   /evidence="ECO:0000259|Pfam:PF00291"
FT   BINDING         91
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605856-50"
FT   BINDING         195..199
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605856-50"
FT   BINDING         283
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605856-50"
FT   MOD_RES         61
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605856-51"
SQ   SEQUENCE   326 AA;  34472 MW;  6BAB10C3C5C3212A CRC64;
     MTDTSTPDTV RKPGHGRVYG SITETIGNTP LVRLNRLTKE RGVDAEILLK LEFFNPISSV
     KDRIGVNMID ALEASGRLKP GGTLVEPTSG NTGIALAFVA AARGYRLILV MPETMSVERR
     KMLAFLGAQL ELTPGAQGMK GAIGRAEELL REIDGAVMPQ QFSNPANPEI HRKTTAEEIW
     NDTQGQLDAF VAGVGTGGTV TGVGEVLKPR LPNLKVFAVE PVDSPVISGG QPGPHKIQGI
     GAGFIPDNLH TDILDGVLKV SNQQAIDTAR DLAKFEGIPG GISTGGNVAA ALELAGRPEF
     QGKRIVTIAC SFAERYISSV LFEGIG
//