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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Methylobacterium extorquens (strain DSM 6343 / CIP 106787 / DM4) (Methylobacterium dichloromethanicum (strain DM4))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotationSAAS annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100ElectrophileUniRule annotation1
Active sitei170Proton acceptorUniRule annotation1
Binding sitei176Substrate; via amide nitrogenUniRule annotation1
Binding sitei215SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotationImported

Keywords - Biological processi

GluconeogenesisUniRule annotationSAAS annotation, GlycolysisUniRule annotationSAAS annotation, Pentose shuntUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Short name:
TPIUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotationImported
Ordered Locus Names:METDI5716Imported
OrganismiMethylobacterium extorquens (strain DSM 6343 / CIP 106787 / DM4) (Methylobacterium dichloromethanicum (strain DM4))Imported
Taxonomic identifieri661410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008070 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliC7C913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15Substrate bindingUniRule annotation3
Regioni236 – 237Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000226414.
KOiK01803.
OMAiWKMNNDL.
OrthoDBiPOG091H02GL.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C7C913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEGRRPLV AGNWKMNGLL SSVPTVEAIR DGLSPALADK IDVLICPPAT
60 70 80 90 100
LISSCVAAVY GSVVAIGGQN LHARPSGAFT GSISAEMFAD LGATYVIVGH
110 120 130 140 150
SERRAYHYET DDGVHAKALG ARRAGLRGII CVGETMEERQ QGRALDIVRA
160 170 180 190 200
QLAIGLPKGA TGADTVIAYE PVWAIGSGRT PTATEIAEVH ASLREMLDKL
210 220 230 240 250
VGEEAHKIRI LYGGSVKPSN ARELMAVENV DGALVGGASL VAEDFLGICR

AYEG
Length:254
Mass (Da):26,563
Last modified:September 22, 2009 - v1
Checksum:i0F35FB416B7D4E0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP103042 Genomic DNA. Translation: CAX27320.1.
RefSeqiWP_003602146.1. NC_012988.1.

Genome annotation databases

EnsemblBacteriaiCAX27320; CAX27320; METDI5716.
KEGGimdi:METDI5716.
PATRICi22529885. VBIMetExt143287_5445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP103042 Genomic DNA. Translation: CAX27320.1.
RefSeqiWP_003602146.1. NC_012988.1.

3D structure databases

ProteinModelPortaliC7C913.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX27320; CAX27320; METDI5716.
KEGGimdi:METDI5716.
PATRICi22529885. VBIMetExt143287_5445.

Phylogenomic databases

HOGENOMiHOG000226414.
KOiK01803.
OMAiWKMNNDL.
OrthoDBiPOG091H02GL.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC7C913_METED
AccessioniPrimary (citable) accession number: C7C913
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.