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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Methylobacterium extorquens (strain DSM 6343 / CIP 106787 / DM4) (Methylobacterium dichloromethanicum (strain DM4))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
Gene namesi
Name:glnDUniRule annotationImported
ORF Names:METD_I0212Imported
OrganismiMethylobacterium extorquens (strain DSM 6343 / CIP 106787 / DM4) (Methylobacterium dichloromethanicum (strain DM4))Imported
Taxonomic identifieri661410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008070 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliC7C6X7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini485 – 607HDInterPro annotationAdd BLAST123
Domaini725 – 802ACTInterPro annotationAdd BLAST78
Domaini835 – 918ACTInterPro annotationAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 367UridylyltransferaseUniRule annotationAdd BLAST367

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatSAAS annotation

Phylogenomic databases

HOGENOMiHOG000261779
KOiK00990
OMAiARICGYF
OrthoDBiPOG091H04HO

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

C7C6X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDPVPVLEK IVASLDRDAR EPTKLRGLLV PELRKVIETG HAEAERLLLK
60 70 80 90 100
ERDGLACAQR LSRLTDAVVR AIYDAVVWRL YPNDNPSTGE HLAIVATGGY
110 120 130 140 150
GRGTMAPGSD IDLLFLLPYK QTAWSESVVE AMLYVLWDLK LKVGHATRSV
160 170 180 190 200
EECLREGRAD MTIRTALLES RFLFGSRSLF EEMVTRFDTE LVIGSASEFV
210 220 230 240 250
DAKLRERDAR VAKAGASRYL VEPNVKDGKG GLRDLNTLFW IAKYTYRVRN
260 270 280 290 300
QAELVHAGLF TPDEYRLFER CEEFLWRVRC HIHFVTGRPE ERLSFGLQPK
310 320 330 340 350
IAERLGFGSR GGLSGVERFM KAYFLIAKDV GDLTAIVCAE LEARHAKRTP
360 370 380 390 400
VLDRWIGRFR DRFRATSIEA EDFWIDNGRV NIRGEDAFER DPVNLIRLFW
410 420 430 440 450
LADRHNLPIH PDAARLANRS LKLITHAVRI DPEANRLFLD ILTSKNAPET
460 470 480 490 500
ILRSMNEAGV LGRFIPEFGR IVAMMQFNMY HHYTVDEHLL RSLGVLAAID
510 520 530 540 550
SGRVRDEHPL ASRLIDTIHN RRALYVAILL HDIAKGRPED HSIAGAAIAR
560 570 580 590 600
KLGPRFGLSQ AETETVSWLV EHHLLMSMTA QSRDLSDRRT IEKFASEVQS
610 620 630 640 650
LERLKLLAIL TVADIKAVGP GVWTAWKGTL LRTLYDETEV VLSGGHSEIA
660 670 680 690 700
RTDRVRLIQM ALREQLSDWN SELFDGYAAR HNQAYWLKVD STRHFKNARF
710 720 730 740 750
LRTVMEEGRT SATTYETDPV RGVTELTVYS PDHPRLLAII TGACATMGGN
760 770 780 790 800
IVDAQIFTTT DGFALDSIFI SRAFERDEDE LRRAGRIATA IERALKGEIK
810 820 830 840 850
IAELVADKHP KQPPKTFLVP PDVSIDNALS SRETVVEITG LDRPGLLYEL
860 870 880 890 900
TTGLNRLSLN ITSAHVATFG ERAVDVFYVT DLTGTRVVQP DRLAMIRAAV
910 920
MEVFASDVAA LRAEGLDALV DSPPPREL
Length:928
Mass (Da):104,425
Last modified:September 22, 2009 - v1
Checksum:iD3C1E4F56083055A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP103042 Genomic DNA Translation: CAX21881.1
RefSeqiWP_012779027.1, NC_012988.1

Genome annotation databases

EnsemblBacteriaiCAX21881; CAX21881; METDI0212
KEGGimdi:METDI0212

Similar proteinsi

Entry informationi

Entry nameiC7C6X7_METED
AccessioniPrimary (citable) accession number: C7C6X7
Entry historyiIntegrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health