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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium dichloromethanicum (strain DM4))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciMEXT661410:GJA1-205-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationSAAS annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Gene namesi
Name:glnDUniRule annotationImported
Ordered Locus Names:METDI0212Imported
OrganismiMethylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium dichloromethanicum (strain DM4))Imported
Taxonomic identifieri661410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000008070: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi661410.METDI0212.

Structurei

3D structure databases

ProteinModelPortaliC7C6X7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini510 – 614105HDUniRule annotationAdd
BLAST
Domaini725 – 80278ACT 1UniRule annotationAdd
BLAST
Domaini835 – 91884ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 367367UridylyltransferaseUniRule annotationAdd
BLAST
Regioni369 – 724356Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 1 HD domain.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains ACT domains.SAAS annotation
Contains HD domain.SAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C7C6X7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFDPVPVLEK IVASLDRDAR EPTKLRGLLV PELRKVIETG HAEAERLLLK
60 70 80 90 100
ERDGLACAQR LSRLTDAVVR AIYDAVVWRL YPNDNPSTGE HLAIVATGGY
110 120 130 140 150
GRGTMAPGSD IDLLFLLPYK QTAWSESVVE AMLYVLWDLK LKVGHATRSV
160 170 180 190 200
EECLREGRAD MTIRTALLES RFLFGSRSLF EEMVTRFDTE LVIGSASEFV
210 220 230 240 250
DAKLRERDAR VAKAGASRYL VEPNVKDGKG GLRDLNTLFW IAKYTYRVRN
260 270 280 290 300
QAELVHAGLF TPDEYRLFER CEEFLWRVRC HIHFVTGRPE ERLSFGLQPK
310 320 330 340 350
IAERLGFGSR GGLSGVERFM KAYFLIAKDV GDLTAIVCAE LEARHAKRTP
360 370 380 390 400
VLDRWIGRFR DRFRATSIEA EDFWIDNGRV NIRGEDAFER DPVNLIRLFW
410 420 430 440 450
LADRHNLPIH PDAARLANRS LKLITHAVRI DPEANRLFLD ILTSKNAPET
460 470 480 490 500
ILRSMNEAGV LGRFIPEFGR IVAMMQFNMY HHYTVDEHLL RSLGVLAAID
510 520 530 540 550
SGRVRDEHPL ASRLIDTIHN RRALYVAILL HDIAKGRPED HSIAGAAIAR
560 570 580 590 600
KLGPRFGLSQ AETETVSWLV EHHLLMSMTA QSRDLSDRRT IEKFASEVQS
610 620 630 640 650
LERLKLLAIL TVADIKAVGP GVWTAWKGTL LRTLYDETEV VLSGGHSEIA
660 670 680 690 700
RTDRVRLIQM ALREQLSDWN SELFDGYAAR HNQAYWLKVD STRHFKNARF
710 720 730 740 750
LRTVMEEGRT SATTYETDPV RGVTELTVYS PDHPRLLAII TGACATMGGN
760 770 780 790 800
IVDAQIFTTT DGFALDSIFI SRAFERDEDE LRRAGRIATA IERALKGEIK
810 820 830 840 850
IAELVADKHP KQPPKTFLVP PDVSIDNALS SRETVVEITG LDRPGLLYEL
860 870 880 890 900
TTGLNRLSLN ITSAHVATFG ERAVDVFYVT DLTGTRVVQP DRLAMIRAAV
910 920
MEVFASDVAA LRAEGLDALV DSPPPREL
Length:928
Mass (Da):104,425
Last modified:September 22, 2009 - v1
Checksum:iD3C1E4F56083055A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP103042 Genomic DNA. Translation: CAX21881.1.
RefSeqiYP_003065946.1. NC_012988.1.

Genome annotation databases

EnsemblBacteriaiCAX21881; CAX21881; METDI0212.
GeneIDi8190687.
KEGGimdi:METDI0212.
PATRICi22519508. VBIMetExt143287_0329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP103042 Genomic DNA. Translation: CAX21881.1.
RefSeqiYP_003065946.1. NC_012988.1.

3D structure databases

ProteinModelPortaliC7C6X7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi661410.METDI0212.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX21881; CAX21881; METDI0212.
GeneIDi8190687.
KEGGimdi:METDI0212.
PATRICi22519508. VBIMetExt143287_0329.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Enzyme and pathway databases

BioCyciMEXT661410:GJA1-205-MONOMER.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 5838 / DM4Imported.

Entry informationi

Entry nameiC7C6X7_METED
AccessioniPrimary (citable) accession number: C7C6X7
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: February 4, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.