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C7C6X7

- C7C6X7_METED

UniProt

C7C6X7 - C7C6X7_METED

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium dichloromethanicum (strain DM4))
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Ligandi

    MagnesiumUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciMEXT661410:GJA1-205-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Gene namesi
    Name:glnDUniRule annotationImported
    Ordered Locus Names:METDI0212Imported
    OrganismiMethylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium dichloromethanicum (strain DM4))Imported
    Taxonomic identifieri661410 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000008070: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi661410.METDI0212.

    Structurei

    3D structure databases

    ProteinModelPortaliC7C6X7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini510 – 614105HDUniRule annotationAdd
    BLAST
    Domaini725 – 80278ACT 1UniRule annotationAdd
    BLAST
    Domaini835 – 91884ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 367367UridylyltransferaseUniRule annotationAdd
    BLAST
    Regioni369 – 724356Uridylyl-removingUniRule annotationAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 1 HD domain.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains ACT domains.SAAS annotation
    Contains HD domain.SAAS annotation

    Keywords - Domaini

    RepeatUniRule annotationSAAS annotation

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261779.
    KOiK00990.
    OMAiLYCLWDM.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C7C6X7-1 [UniParc]FASTAAdd to Basket

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    MFDPVPVLEK IVASLDRDAR EPTKLRGLLV PELRKVIETG HAEAERLLLK    50
    ERDGLACAQR LSRLTDAVVR AIYDAVVWRL YPNDNPSTGE HLAIVATGGY 100
    GRGTMAPGSD IDLLFLLPYK QTAWSESVVE AMLYVLWDLK LKVGHATRSV 150
    EECLREGRAD MTIRTALLES RFLFGSRSLF EEMVTRFDTE LVIGSASEFV 200
    DAKLRERDAR VAKAGASRYL VEPNVKDGKG GLRDLNTLFW IAKYTYRVRN 250
    QAELVHAGLF TPDEYRLFER CEEFLWRVRC HIHFVTGRPE ERLSFGLQPK 300
    IAERLGFGSR GGLSGVERFM KAYFLIAKDV GDLTAIVCAE LEARHAKRTP 350
    VLDRWIGRFR DRFRATSIEA EDFWIDNGRV NIRGEDAFER DPVNLIRLFW 400
    LADRHNLPIH PDAARLANRS LKLITHAVRI DPEANRLFLD ILTSKNAPET 450
    ILRSMNEAGV LGRFIPEFGR IVAMMQFNMY HHYTVDEHLL RSLGVLAAID 500
    SGRVRDEHPL ASRLIDTIHN RRALYVAILL HDIAKGRPED HSIAGAAIAR 550
    KLGPRFGLSQ AETETVSWLV EHHLLMSMTA QSRDLSDRRT IEKFASEVQS 600
    LERLKLLAIL TVADIKAVGP GVWTAWKGTL LRTLYDETEV VLSGGHSEIA 650
    RTDRVRLIQM ALREQLSDWN SELFDGYAAR HNQAYWLKVD STRHFKNARF 700
    LRTVMEEGRT SATTYETDPV RGVTELTVYS PDHPRLLAII TGACATMGGN 750
    IVDAQIFTTT DGFALDSIFI SRAFERDEDE LRRAGRIATA IERALKGEIK 800
    IAELVADKHP KQPPKTFLVP PDVSIDNALS SRETVVEITG LDRPGLLYEL 850
    TTGLNRLSLN ITSAHVATFG ERAVDVFYVT DLTGTRVVQP DRLAMIRAAV 900
    MEVFASDVAA LRAEGLDALV DSPPPREL 928
    Length:928
    Mass (Da):104,425
    Last modified:September 22, 2009 - v1
    Checksum:iD3C1E4F56083055A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FP103042 Genomic DNA. Translation: CAX21881.1.
    RefSeqiWP_012779027.1. NC_012988.1.
    YP_003065946.1. NC_012988.1.

    Genome annotation databases

    EnsemblBacteriaiCAX21881; CAX21881; METDI0212.
    GeneIDi8190687.
    KEGGimdi:METDI0212.
    PATRICi22519508. VBIMetExt143287_0329.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FP103042 Genomic DNA. Translation: CAX21881.1 .
    RefSeqi WP_012779027.1. NC_012988.1.
    YP_003065946.1. NC_012988.1.

    3D structure databases

    ProteinModelPortali C7C6X7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 661410.METDI0212.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAX21881 ; CAX21881 ; METDI0212 .
    GeneIDi 8190687.
    KEGGi mdi:METDI0212.
    PATRICi 22519508. VBIMetExt143287_0329.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261779.
    KOi K00990.
    OMAi LYCLWDM.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci MEXT661410:GJA1-205-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 5838 / DM4Imported.

    Entry informationi

    Entry nameiC7C6X7_METED
    AccessioniPrimary (citable) accession number: C7C6X7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

    External Data

    Dasty 3