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C7C422

- BLAN1_KLEPN

UniProt

C7C422 - BLAN1_KLEPN

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Protein

Beta-lactamase NDM-1

Gene

blaNDM-1

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Confers resistance to many beta-lactam antibiotics, including some carbapenems. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin.1 Publication

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.1 Publication

Cofactori

Binds 2 zinc ions per subunit.By similarity

Kineticsi

No activity detected against the monobactam aztreonam or the beta-lactamase inhibitor clavulanic acid.

  1. KM=8 µM for cefuroxime1 Publication
  2. KM=10 µM for cefotaxime1 Publication
  3. KM=10 µM for cephalothin1 Publication
  4. KM=12 µM for piperacillin1 Publication
  5. KM=16 µM for penicillin G1 Publication
  6. KM=22 µM for ampicillin1 Publication
  7. KM=49 µM for cefoxitin1 Publication
  8. KM=49 µM for meropenem1 Publication
  9. KM=77 µM for cefepime1 Publication
  10. KM=94 µM for imipenem1 Publication
  11. KM=181 µM for ceftazidime1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201Zinc 1By similarity
Metal bindingi122 – 1221Zinc 1By similarity
Metal bindingi124 – 1241Zinc 2By similarity
Metal bindingi189 – 1891Zinc 1By similarity
Metal bindingi208 – 2081Zinc 2By similarity
Metal bindingi250 – 2501Zinc 2By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase NDM-1 (EC:3.5.2.6)
Short name:
NDM-1
Alternative name(s):
Metallo-beta-lactamase NDM-1
Gene namesi
Name:blaNDM-1
Encoded oniPlasmid pKpANDM-10 Publication
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Subcellular locationi

Periplasm Curated
Note: The predicted signal sequence is not very convincing.

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 270242Beta-lactamase NDM-1PRO_0000400316Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 363Combined sources
Beta strandi43 – 464Combined sources
Beta strandi49 – 557Combined sources
Beta strandi58 – 6710Combined sources
Turni68 – 703Combined sources
Beta strandi71 – 8212Combined sources
Beta strandi85 – 906Combined sources
Helixi95 – 10814Combined sources
Beta strandi113 – 1175Combined sources
Beta strandi120 – 1223Combined sources
Helixi123 – 1264Combined sources
Helixi129 – 1346Combined sources
Beta strandi138 – 1425Combined sources
Helixi143 – 1486Combined sources
Helixi149 – 1513Combined sources
Beta strandi158 – 1614Combined sources
Beta strandi167 – 1693Combined sources
Helixi171 – 1733Combined sources
Beta strandi180 – 1834Combined sources
Beta strandi187 – 1904Combined sources
Beta strandi195 – 1984Combined sources
Turni199 – 2024Combined sources
Beta strandi203 – 2053Combined sources
Turni207 – 2093Combined sources
Helixi221 – 2233Combined sources
Turni226 – 2283Combined sources
Helixi229 – 23911Combined sources
Beta strandi245 – 2473Combined sources
Beta strandi249 – 2513Combined sources
Helixi257 – 26711Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PG4X-ray2.00A49-270[»]
3Q6XX-ray1.30A/B29-270[»]
3RKJX-ray2.00A/B39-270[»]
3RKKX-ray2.35A/B39-270[»]
3SBLX-ray2.31A39-270[»]
3SFPX-ray2.27A/B/C/D39-270[»]
3SPUX-ray2.10A/B/C/D/E27-270[»]
3SRXX-ray2.50A/B37-270[»]
3ZR9X-ray1.91A42-270[»]
4EXSX-ray2.40A/B1-270[»]
4EXYX-ray1.47A/B1-270[»]
4EY2X-ray1.17A/B1-270[»]
4EYBX-ray1.16A/B1-270[»]
4EYFX-ray1.80A/B1-270[»]
4EYLX-ray1.90A/B1-270[»]
4GYQX-ray1.35A/B/C/D31-270[»]
4GYUX-ray1.80A31-270[»]
4H0DX-ray1.50A/B31-270[»]
4HKYX-ray2.00A/B31-270[»]
4HL1X-ray1.50A/B31-270[»]
4HL2X-ray1.05A/B31-270[»]
4U4LX-ray1.90A/B/C/D27-270[»]
ProteinModelPortaliC7C422.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiC7C422.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Beta-lactamas-like.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C7C422-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELPNIMHPV AKLSTALAAA LMLSGCMPGE IRPTIGQQME TGDQRFGDLV
60 70 80 90 100
FRQLAPNVWQ HTSYLDMPGF GAVASNGLIV RDGGRVLVVD TAWTDDQTAQ
110 120 130 140 150
ILNWIKQEIN LPVALAVVTH AHQDKMGGMD ALHAAGIATY ANALSNQLAP
160 170 180 190 200
QEGMVAAQHS LTFAANGWVE PATAPNFGPL KVFYPGPGHT SDNITVGIDG
210 220 230 240 250
TDIAFGGCLI KDSKAKSLGN LGDADTEHYA ASARAFGAAF PKASMIVMSH
260 270
SAPDSRAAIT HTARMADKLR
Length:270
Mass (Da):28,499
Last modified:September 22, 2009 - v1
Checksum:i1B6009120B945F14
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN396876 Genomic DNA. Translation: CAZ39946.1.
RefSeqiYP_005352173.1. NC_016980.1.
YP_006958736.1. NC_019153.1.
YP_006959150.1. NC_019158.1.
YP_006959255.1. NC_019162.1.
YP_006959318.1. NC_019163.1.
YP_007195502.1. NC_019889.1.
YP_007641427.1. NC_020811.1.
YP_007936899.1. NC_021180.1.
YP_008719663.1. NC_022609.1.
YP_009023060.1. NC_023908.1.

Genome annotation databases

GeneIDi11934636.
13913959.
13914060.
13914125.
13914405.
14258327.
14858116.
15407950.
17373266.
18983573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN396876 Genomic DNA. Translation: CAZ39946.1 .
RefSeqi YP_005352173.1. NC_016980.1.
YP_006958736.1. NC_019153.1.
YP_006959150.1. NC_019158.1.
YP_006959255.1. NC_019162.1.
YP_006959318.1. NC_019163.1.
YP_007195502.1. NC_019889.1.
YP_007641427.1. NC_020811.1.
YP_007936899.1. NC_021180.1.
YP_008719663.1. NC_022609.1.
YP_009023060.1. NC_023908.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3PG4 X-ray 2.00 A 49-270 [» ]
3Q6X X-ray 1.30 A/B 29-270 [» ]
3RKJ X-ray 2.00 A/B 39-270 [» ]
3RKK X-ray 2.35 A/B 39-270 [» ]
3SBL X-ray 2.31 A 39-270 [» ]
3SFP X-ray 2.27 A/B/C/D 39-270 [» ]
3SPU X-ray 2.10 A/B/C/D/E 27-270 [» ]
3SRX X-ray 2.50 A/B 37-270 [» ]
3ZR9 X-ray 1.91 A 42-270 [» ]
4EXS X-ray 2.40 A/B 1-270 [» ]
4EXY X-ray 1.47 A/B 1-270 [» ]
4EY2 X-ray 1.17 A/B 1-270 [» ]
4EYB X-ray 1.16 A/B 1-270 [» ]
4EYF X-ray 1.80 A/B 1-270 [» ]
4EYL X-ray 1.90 A/B 1-270 [» ]
4GYQ X-ray 1.35 A/B/C/D 31-270 [» ]
4GYU X-ray 1.80 A 31-270 [» ]
4H0D X-ray 1.50 A/B 31-270 [» ]
4HKY X-ray 2.00 A/B 31-270 [» ]
4HL1 X-ray 1.50 A/B 31-270 [» ]
4HL2 X-ray 1.05 A/B 31-270 [» ]
4U4L X-ray 1.90 A/B/C/D 27-270 [» ]
ProteinModelPortali C7C422.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1667695.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 11934636.
13913959.
13914060.
13914125.
13914405.
14258327.
14858116.
15407950.
17373266.
18983573.

Miscellaneous databases

EvolutionaryTracei C7C422.

Family and domain databases

Gene3Di 3.60.15.10. 1 hit.
InterProi IPR001279. Beta-lactamas-like.
[Graphical view ]
Pfami PF00753. Lactamase_B. 1 hit.
[Graphical view ]
SMARTi SM00849. Lactamase_B. 1 hit.
[Graphical view ]
SUPFAMi SSF56281. SSF56281. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India."
    Yong D., Toleman M.A., Giske C.G., Cho H.S., Sundman K., Lee K., Walsh T.R.
    Antimicrob. Agents Chemother. 53:5046-5054(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: KP-05-506.
  2. Cited for: EPIDEMIOLOGY.
  3. "Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase -United States, 2010."
    Limbago B., Kallen A.
    MMWR Morb. Mortal. Wkly. Rep. 59:750-750(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: EPIDEMIOLOGY.

Entry informationi

Entry nameiBLAN1_KLEPN
AccessioniPrimary (citable) accession number: C7C422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: September 22, 2009
Last modified: October 29, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Enterobacteriaceae carrying this gene (identified by PCR) have been isolated in Sweden, India, Pakistan, Bangladesh, England, Scotland, Northern Ireland, Australia and the USA. The organisms they were identified in were K.pneumoniae, Enterobacter cloacae, E.coli, Proteus spp, Citrobacter freundii, Morganella morganii, Providencia spp and Klebsille oxytoca. In India most isolates were from community-acquired infections rather than hospital-acquired infections, indicating the gene is widespread in the environment.

Caution

Transfer of a plasmid encoding this gene has been detected between bacteria within a patient (between K.pneumoniae and E.coli). Additionally the gene may be encoded within a transposon.Curated

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3