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C7C422

- BLAN1_KLEPN

UniProt

C7C422 - BLAN1_KLEPN

Protein

Beta-lactamase NDM-1

Gene

blaNDM-1

Organism
Klebsiella pneumoniae
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Confers resistance to many beta-lactam antibiotics, including some carbapenems. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin.1 Publication

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.1 Publication

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Kineticsi

    No activity detected against the monobactam aztreonam or the beta-lactamase inhibitor clavulanic acid.

    1. KM=8 µM for cefuroxime1 Publication
    2. KM=10 µM for cefotaxime1 Publication
    3. KM=10 µM for cephalothin1 Publication
    4. KM=12 µM for piperacillin1 Publication
    5. KM=16 µM for penicillin G1 Publication
    6. KM=22 µM for ampicillin1 Publication
    7. KM=49 µM for cefoxitin1 Publication
    8. KM=49 µM for meropenem1 Publication
    9. KM=77 µM for cefepime1 Publication
    10. KM=94 µM for imipenem1 Publication
    11. KM=181 µM for ceftazidime1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi120 – 1201Zinc 1By similarity
    Metal bindingi122 – 1221Zinc 1By similarity
    Metal bindingi124 – 1241Zinc 2By similarity
    Metal bindingi189 – 1891Zinc 1By similarity
    Metal bindingi208 – 2081Zinc 2By similarity
    Metal bindingi250 – 2501Zinc 2By similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase NDM-1 (EC:3.5.2.6)
    Short name:
    NDM-1
    Alternative name(s):
    Metallo-beta-lactamase NDM-1
    Gene namesi
    Name:blaNDM-1
    Encoded oniPlasmid pKpANDM-10 Publication
    OrganismiKlebsiella pneumoniae
    Taxonomic identifieri573 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    Subcellular locationi

    Periplasm Curated
    Note: The predicted signal sequence is not very convincing.

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 270242Beta-lactamase NDM-1PRO_0000400316Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Beta strandi43 – 464
    Beta strandi49 – 557
    Beta strandi58 – 6710
    Turni68 – 703
    Beta strandi71 – 8212
    Beta strandi85 – 906
    Helixi95 – 10814
    Beta strandi113 – 1175
    Beta strandi120 – 1223
    Helixi123 – 1264
    Helixi129 – 1346
    Beta strandi138 – 1425
    Helixi143 – 1486
    Helixi149 – 1513
    Beta strandi158 – 1614
    Beta strandi167 – 1693
    Helixi171 – 1733
    Beta strandi180 – 1834
    Beta strandi187 – 1904
    Beta strandi195 – 1984
    Turni199 – 2024
    Beta strandi203 – 2053
    Turni207 – 2093
    Helixi221 – 2233
    Turni226 – 2283
    Helixi229 – 23911
    Beta strandi245 – 2473
    Beta strandi249 – 2513
    Helixi257 – 26711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3PG4X-ray2.00A49-270[»]
    3Q6XX-ray1.30A/B29-270[»]
    3RKJX-ray2.00A/B39-270[»]
    3RKKX-ray2.35A/B39-270[»]
    3SBLX-ray2.31A39-270[»]
    3SFPX-ray2.27A/B/C/D39-270[»]
    3SPUX-ray2.10A/B/C/D/E27-270[»]
    3SRXX-ray2.50A/B37-270[»]
    3ZR9X-ray1.91A42-270[»]
    4EXSX-ray2.40A/B1-270[»]
    4EXYX-ray1.47A/B1-270[»]
    4EY2X-ray1.17A/B1-270[»]
    4EYBX-ray1.16A/B1-270[»]
    4EYFX-ray1.80A/B1-270[»]
    4EYLX-ray1.90A/B1-270[»]
    4GYQX-ray1.35A/B/C/D31-270[»]
    4GYUX-ray1.80A31-270[»]
    4H0DX-ray1.50A/B31-270[»]
    4HKYX-ray2.00A/B31-270[»]
    4HL1X-ray1.50A/B31-270[»]
    4HL2X-ray1.05A/B31-270[»]
    ProteinModelPortaliC7C422.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiC7C422.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Beta-lactamas-like.
    [Graphical view]
    PfamiPF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C7C422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELPNIMHPV AKLSTALAAA LMLSGCMPGE IRPTIGQQME TGDQRFGDLV    50
    FRQLAPNVWQ HTSYLDMPGF GAVASNGLIV RDGGRVLVVD TAWTDDQTAQ 100
    ILNWIKQEIN LPVALAVVTH AHQDKMGGMD ALHAAGIATY ANALSNQLAP 150
    QEGMVAAQHS LTFAANGWVE PATAPNFGPL KVFYPGPGHT SDNITVGIDG 200
    TDIAFGGCLI KDSKAKSLGN LGDADTEHYA ASARAFGAAF PKASMIVMSH 250
    SAPDSRAAIT HTARMADKLR 270
    Length:270
    Mass (Da):28,499
    Last modified:September 22, 2009 - v1
    Checksum:i1B6009120B945F14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN396876 Genomic DNA. Translation: CAZ39946.1.
    RefSeqiYP_005352173.1. NC_016980.1.
    YP_006958736.1. NC_019153.1.
    YP_006959150.1. NC_019158.1.
    YP_006959255.1. NC_019162.1.
    YP_006959318.1. NC_019163.1.
    YP_007195502.1. NC_019889.1.
    YP_007641427.1. NC_020811.1.
    YP_007936899.1. NC_021180.1.
    YP_008719663.1. NC_022609.1.
    YP_009023060.1. NC_023908.1.

    Genome annotation databases

    GeneIDi11934636.
    13913959.
    13914060.
    13914125.
    13914405.
    14258327.
    14858116.
    15407950.
    17373266.
    18983573.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN396876 Genomic DNA. Translation: CAZ39946.1 .
    RefSeqi YP_005352173.1. NC_016980.1.
    YP_006958736.1. NC_019153.1.
    YP_006959150.1. NC_019158.1.
    YP_006959255.1. NC_019162.1.
    YP_006959318.1. NC_019163.1.
    YP_007195502.1. NC_019889.1.
    YP_007641427.1. NC_020811.1.
    YP_007936899.1. NC_021180.1.
    YP_008719663.1. NC_022609.1.
    YP_009023060.1. NC_023908.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3PG4 X-ray 2.00 A 49-270 [» ]
    3Q6X X-ray 1.30 A/B 29-270 [» ]
    3RKJ X-ray 2.00 A/B 39-270 [» ]
    3RKK X-ray 2.35 A/B 39-270 [» ]
    3SBL X-ray 2.31 A 39-270 [» ]
    3SFP X-ray 2.27 A/B/C/D 39-270 [» ]
    3SPU X-ray 2.10 A/B/C/D/E 27-270 [» ]
    3SRX X-ray 2.50 A/B 37-270 [» ]
    3ZR9 X-ray 1.91 A 42-270 [» ]
    4EXS X-ray 2.40 A/B 1-270 [» ]
    4EXY X-ray 1.47 A/B 1-270 [» ]
    4EY2 X-ray 1.17 A/B 1-270 [» ]
    4EYB X-ray 1.16 A/B 1-270 [» ]
    4EYF X-ray 1.80 A/B 1-270 [» ]
    4EYL X-ray 1.90 A/B 1-270 [» ]
    4GYQ X-ray 1.35 A/B/C/D 31-270 [» ]
    4GYU X-ray 1.80 A 31-270 [» ]
    4H0D X-ray 1.50 A/B 31-270 [» ]
    4HKY X-ray 2.00 A/B 31-270 [» ]
    4HL1 X-ray 1.50 A/B 31-270 [» ]
    4HL2 X-ray 1.05 A/B 31-270 [» ]
    ProteinModelPortali C7C422.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1667695.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 11934636.
    13913959.
    13914060.
    13914125.
    13914405.
    14258327.
    14858116.
    15407950.
    17373266.
    18983573.

    Miscellaneous databases

    EvolutionaryTracei C7C422.

    Family and domain databases

    Gene3Di 3.60.15.10. 1 hit.
    InterProi IPR001279. Beta-lactamas-like.
    [Graphical view ]
    Pfami PF00753. Lactamase_B. 1 hit.
    [Graphical view ]
    SMARTi SM00849. Lactamase_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56281. SSF56281. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India."
      Yong D., Toleman M.A., Giske C.G., Cho H.S., Sundman K., Lee K., Walsh T.R.
      Antimicrob. Agents Chemother. 53:5046-5054(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: KP-05-506.
    2. Cited for: EPIDEMIOLOGY.
    3. "Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase -United States, 2010."
      Limbago B., Kallen A.
      MMWR Morb. Mortal. Wkly. Rep. 59:750-750(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: EPIDEMIOLOGY.

    Entry informationi

    Entry nameiBLAN1_KLEPN
    AccessioniPrimary (citable) accession number: C7C422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Enterobacteriaceae carrying this gene (identified by PCR) have been isolated in Sweden, India, Pakistan, Bangladesh, England, Scotland, Northern Ireland, Australia and the USA. The organisms they were identified in were K.pneumoniae, Enterobacter cloacae, E.coli, Proteus spp, Citrobacter freundii, Morganella morganii, Providencia spp and Klebsille oxytoca. In India most isolates were from community-acquired infections rather than hospital-acquired infections, indicating the gene is widespread in the environment.

    Caution

    Transfer of a plasmid encoding this gene has been detected between bacteria within a patient (between K.pneumoniae and E.coli). Additionally the gene may be encoded within a transposon.Curated

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3