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C7C422 (BLAN1_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase NDM-1

Short name=NDM-1
EC=3.5.2.6
Alternative name(s):
Metallo-beta-lactamase NDM-1
Gene names
Name:blaNDM-1
Encoded onPlasmid pKpANDM-1
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Confers resistance to many beta-lactam antibiotics, including some carbapenems. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin. Ref.1

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid. Ref.1

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Monomer. Ref.1

Subcellular location

Periplasm Potential. Note: The predicted signal sequence is not very convincing.

Miscellaneous

Enterobacteriaceae carrying this gene (identified by PCR) have been isolated in Sweden, India, Pakistan, Bangladesh, England, Scotland, Northern Ireland, Australia and the USA. The organisms they were identified in were K.pneumoniae, Enterobacter cloacae, E.coli, Proteus spp, Citrobacter freundii, Morganella morganii, Providencia spp and Klebsille oxytoca. In India most isolates were from community-acquired infections rather than hospital-acquired infections, indicating the gene is widespread in the environment.

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family.

Caution

Transfer of a plasmid encoding this gene has been detected between bacteria within a patient (between K.pneumoniae and E.coli). Additionally the gene may be encoded within a transposon.

Biophysicochemical properties

Kinetic parameters:

No activity detected against the monobactam aztreonam or the beta-lactamase inhibitor clavulanic acid.

KM=8 µM for cefuroxime Ref.1

KM=10 µM for cefotaxime

KM=10 µM for cephalothin

KM=12 µM for piperacillin

KM=16 µM for penicillin G

KM=22 µM for ampicillin

KM=49 µM for cefoxitin

KM=49 µM for meropenem

KM=77 µM for cefepime

KM=94 µM for imipenem

KM=181 µM for ceftazidime

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentPeriplasm
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processresponse to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 270242Beta-lactamase NDM-1
PRO_0000400316

Sites

Metal binding1201Zinc 1 By similarity
Metal binding1221Zinc 1 By similarity
Metal binding1241Zinc 2 By similarity
Metal binding1891Zinc 1 By similarity
Metal binding2081Zinc 2 By similarity
Metal binding2501Zinc 2 By similarity

Secondary structure

..................................................... 270
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
C7C422 [UniParc].

Last modified September 22, 2009. Version 1.
Checksum: 1B6009120B945F14

FASTA27028,499
        10         20         30         40         50         60 
MELPNIMHPV AKLSTALAAA LMLSGCMPGE IRPTIGQQME TGDQRFGDLV FRQLAPNVWQ 

        70         80         90        100        110        120 
HTSYLDMPGF GAVASNGLIV RDGGRVLVVD TAWTDDQTAQ ILNWIKQEIN LPVALAVVTH 

       130        140        150        160        170        180 
AHQDKMGGMD ALHAAGIATY ANALSNQLAP QEGMVAAQHS LTFAANGWVE PATAPNFGPL 

       190        200        210        220        230        240 
KVFYPGPGHT SDNITVGIDG TDIAFGGCLI KDSKAKSLGN LGDADTEHYA ASARAFGAAF 

       250        260        270 
PKASMIVMSH SAPDSRAAIT HTARMADKLR 

« Hide

References

[1]"Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India."
Yong D., Toleman M.A., Giske C.G., Cho H.S., Sundman K., Lee K., Walsh T.R.
Antimicrob. Agents Chemother. 53:5046-5054(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: KP-05-506.
[2]"Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study."
Kumarasamy K.K., Toleman M.A., Walsh T.R., Bagaria J., Butt F., Balakrishnan R., Chaudhary U., Doumith M., Giske C.G., Irfan S., Krishnan P., Kumar A.V., Maharjan S., Mushtaq S., Noorie T., Paterson D.L., Pearson A., Perry C. expand/collapse author list , Pike R., Rao B., Ray U., Sarma J.B., Sharma M., Sheridan E., Thirunarayan M.A., Turton J., Upadhyay S., Warner M., Welfare W., Livermore D.M., Woodford N.
Lancet Infect. Dis. 10:597-602(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: EPIDEMIOLOGY.
[3]"Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase -United States, 2010."
Limbago B., Kallen A.
MMWR Morb. Mortal. Wkly. Rep. 59:750-750(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: EPIDEMIOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN396876 Genomic DNA. Translation: CAZ39946.1.
RefSeqYP_005352173.1. NC_016980.1.
YP_006958736.1. NC_019153.1.
YP_006959150.1. NC_019158.1.
YP_006959255.1. NC_019162.1.
YP_006959318.1. NC_019163.1.
YP_007195502.1. NC_019889.1.
YP_007641427.1. NC_020811.1.
YP_007936899.1. NC_021180.1.
YP_008719663.1. NC_022609.1.
YP_009023060.1. NC_023908.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3PG4X-ray2.00A49-270[»]
3Q6XX-ray1.30A/B29-270[»]
3RKJX-ray2.00A/B39-270[»]
3RKKX-ray2.35A/B39-270[»]
3SBLX-ray2.31A39-270[»]
3SFPX-ray2.27A/B/C/D39-270[»]
3SPUX-ray2.10A/B/C/D/E27-270[»]
3SRXX-ray2.50A/B37-270[»]
3ZR9X-ray1.91A42-270[»]
4EXSX-ray2.40A/B1-270[»]
4EXYX-ray1.47A/B1-270[»]
4EY2X-ray1.17A/B1-270[»]
4EYBX-ray1.16A/B1-270[»]
4EYFX-ray1.80A/B1-270[»]
4EYLX-ray1.90A/B1-270[»]
4GYQX-ray1.35A/B/C/D31-270[»]
4GYUX-ray1.80A31-270[»]
4H0DX-ray1.50A/B31-270[»]
4HKYX-ray2.00A/B31-270[»]
4HL1X-ray1.50A/B31-270[»]
4HL2X-ray1.05A/B31-270[»]
ProteinModelPortalC7C422.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL1667695.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11934636.
13913959.
13914060.
13914125.
13914405.
14258327.
14858116.
15407950.
17373266.
18983573.

Family and domain databases

Gene3D3.60.15.10. 1 hit.
InterProIPR001279. Beta-lactamas-like.
[Graphical view]
PfamPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMSSF56281. SSF56281. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceC7C422.

Entry information

Entry nameBLAN1_KLEPN
AccessionPrimary (citable) accession number: C7C422
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: September 22, 2009
Last modified: July 9, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references