C7C1C0 (C7C1C0_HELPB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049 EMBL CAX30251.1
Ordered Locus Names:	HELPY_1550 EMBL CAX30251.1

Organism

Helicobacter pylori (strain B38) [Complete proteome] [HAMAP] EMBL CAX30251.1

Taxonomic identifier

592205 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049 RuleBase RU003489
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 RuleBase RU003489 EMBL CAX30251.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	40 – 50	11	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	574 – 578	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	577	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	C7C1C0 [UniParc]. Last modified September 22, 2009. Version 1. Checksum: 55221F7BDC90B27E	FASTA	808	93,254
	10 20 30 40 50 60 MMDFINIIEK KWQEFWWKNK SFEPKDDFNL PKKYILSMLP YPSGEIHMGH VRNYTIGDAL 70 80 90 100 110 120 ARYYRLHHYN VLHPMGFDSF GMPAENAAIK HGIHPKTWTY ENIEAMQKEF EALGFSFSKN 130 140 150 160 170 180 REFATSDPDY TKFEQQFFID LWEKGLIYRK KAMLNWCPND KTVLANEQVI DGKCWRCDTE 190 200 210 220 230 240 VVQKELYQYY LKITNYAEEL LKDLETLEDH WPSQVLIMQK NWIGKSSGLQ FGFKIADECL 250 260 270 280 290 300 KACNNIQEIE VFTTRADTIY GVTYIAIAPE HPLVEHAIKQ VSQEDSKMIK AILNTTQRER 310 320 330 340 350 360 ALEKKGAFLG IYAIHPLTKQ KIPVWVANFA LANYGSGALM GVPACDERDF EFANLYHIPI 370 380 390 400 410 420 KVITQSPQNL PHTKEEVLKN SGEWSNLSSS VAREKIIAYF EKENLGKRVI NYRLQDWGVS 430 440 450 460 470 480 RQRYWGAPIP MIHCKHCGIV PETQLPVTLP EDIVIDGEGN PLERHASWKF AQCPKCHKDA 490 500 510 520 530 540 LRETDTMDTF IQSSWYFLRY TTPKNQRENQ AFDQNYLKYF MPVDTYIGGI EHAILHLLYA 550 560 570 580 590 600 RFFTKALRDL GYLHLDEPFK QLITQGMVLK NGAKMSKSKG NVVSPKEILK KYGADAARLF 610 620 630 640 650 660 ILFAAPPAKE LEWNDSALEG AHRFIKRLYD KASAITPTTS KPEFKGVSLN EAQKLGRKKV 670 680 690 700 710 720 YEALKKSHEI FNKAESAYAF NTLIASCMEA LNALNAQTNE QILCEGYFVL LQILEPIIPH 730 740 750 760 770 780 TAWELSERLF KRENFKPIAI DESALMEDFM TLGLTINGKR RAELKVNINA SKEEIIILAK 790 800 KELEKYLEKA SVKKEIYVPN KLVNFVVA

References

[1]

"From array-based hybridization of Helicobacter pylori isolates to the complete genome sequence of an isolate associated with MALT lymphoma." Thiberge J.M., Boursaux-Eude C., Lehours P., Dillies M.A., Creno S., Coppee J.Y., Rouy Z., Lajus A., Ma L., Burucoa C., Ruskone-Foumestraux A., Courillon-Mallet A., De Reuse H., Gomperts Boneca I., Lamarque D., Megraud F., Delchier J.C., Medigue C. , Bouchier C., Labigne A., Raymond J. BMC Genomics 11:368-368(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B38 EMBL CAX30251.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM991728 Genomic DNA. Translation: CAX30251.1.
RefSeq	YP_003058186.1. NC_012973.1.
3D structure databases
ProteinModelPortal	C7C1C0.
ModBase	Search...
Protein-protein interaction databases
STRING	592205.HELPY_1550.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAX30251; CAX30251; HELPY_1550.
GeneID	8208297.
KEGG	hpb:HELPY_1550.
PATRIC	20597775. VBIHelPyl144313_1538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200747.
KO	K01869.
OMA	GWPEQVK.
ProtClustDB	PRK00390.
Enzyme and pathway databases
BioCyc	HPYL592205:GJAG-1530-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 2 hits.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF00133. tRNA-synt_1. 1 hit. PF13603. tRNA-synt_1_2. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C7C1C0_HELPB

Accession

Primary (citable) accession number: C7C1C0

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)