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C7BK35 (C7BK35_PHOAA) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD 1 HAMAP MF_00336
EC=6.3.3.3 HAMAP MF_00336
Alternative name(s):
DTB synthetase 1 HAMAP MF_00336
Dethiobiotin synthase 1 HAMAP MF_00336
Gene names
Name:ynfK EMBL CAQ84275.1
Synonyms:bioD1 HAMAP MF_00336
Ordered Locus Names:PAU_02183
OrganismPhotorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) (Xenorhabdus luminescens (strain 2)) [Complete proteome] [HAMAP]
Taxonomic identifier553480 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336 SAAS SAAS004472

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336 SAAS SAAS004472

Cofactor

Magnesium By similarity. HAMAP MF_00336 SAAS SAAS004472

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336 SAAS SAAS004472

Subunit structure

Homodimer By similarity. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336 SAAS SAAS004472.

Sequence similarities

Belongs to the dethiobiotin synthetase family. HAMAP MF_00336

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding13 – 186ATP By similarity HAMAP MF_00336
Nucleotide binding109 – 1124ATP By similarity HAMAP MF_00336
Nucleotide binding169 – 1702ATP By similarity HAMAP MF_00336
Nucleotide binding198 – 2003ATP By similarity HAMAP MF_00336

Sites

Metal binding131Magnesium 1 By similarity HAMAP MF_00336
Metal binding171Magnesium 2 By similarity HAMAP MF_00336
Metal binding551Magnesium 2 By similarity HAMAP MF_00336
Metal binding1091Magnesium 2 By similarity HAMAP MF_00336
Binding site421Substrate By similarity HAMAP MF_00336
Binding site551ATP By similarity HAMAP MF_00336
Binding site2051ATP By similarity HAMAP MF_00336

Sequences

Sequence LengthMass (Da)Tools
C7BK35 [UniParc].

Last modified September 22, 2009. Version 1.
Checksum: E084C6F399007FA6

FASTA22325,084
        10         20         30         40         50         60 
MLTRLFVTGT DTDIGKTVVT RALLQVFNRD GATAVGYKPI ATEGYETEEG MRNRDAVVMQ 

        70         80         90        100        110        120 
KSSPVQLRYE EINPVIWKNS YCEGNQIDFE LMKKGLNNLS NKADRVIIEG NGGWRVLLND 

       130        140        150        160        170        180 
ETFYFDWVIR EKIPVILVVG IQPGCVNHAI LTAQAIINDG LPLVGWIANR INPGLSHYIK 

       190        200        210        220 
VLERLRCHIN APQLGEIPYV LRPEEKDLSC YLDISAILKQ TGK

« Hide

References

[1]"Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens."
Wilkinson P., Waterfield N.R., Crossman L., Corton C., Sanchez-Contreras M., Vlisidou I., Barron A., Bignell A., Clark L., Ormond D., Mayho M., Bason N., Smith F., Simmonds M., Churcher C., Harris D., Thompson N.R., Quail M., Parkhill J., ffrench-Constant R.H.
BMC Genomics 10:302-302(2009) [PubMed: 19583835] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM162591 Genomic DNA. Translation: CAQ84275.1.
RefSeqYP_003041019.1. NC_012962.1.

3D structure databases

ProteinModelPortalC7BK35.
ModBaseSearch...

Protein-protein interaction databases

STRINGC7BK35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8166333.
GenomeReviewsGene locus PAU_02183 in contig FM162591_GR.
PATRIC20498252. VBIPhoAsy71438_2394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAINPGLSH.
ProtClustDBCLSK2394752.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC7BK35_PHOAA
AccessionPrimary (citable) accession number: C7BK35
Entry history
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: December 14, 2011
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info