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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) (Xenorhabdus luminescens (strain 2))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotationSAAS annotation

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38NADUniRule annotation1
Binding sitei39NADPUniRule annotation1
Active sitei159Proton donor/acceptorUniRule annotation1
Binding sitei160SubstrateUniRule annotation1
Active sitei163Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17NAD(P)UniRule annotation6
Nucleotide bindingi102 – 104NAD(P)UniRule annotation3
Nucleotide bindingi126 – 129NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

NADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPASY291112:GJQ0-575-MONOMER.
UniPathwayiUPA00034; UER00018.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotationSAAS annotation (EC:1.17.1.8UniRule annotationSAAS annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotationImported
Ordered Locus Names:PAU_00560Imported
OrganismiPhotorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) (Xenorhabdus luminescens (strain 2))Imported
Taxonomic identifieri553480 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaePhotorhabdus
Proteomesi
  • UP000002747 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi553480.PAU_00560.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 129DapB_NInterPro annotationAdd BLAST124
Domaini132 – 268DapB_CInterPro annotationAdd BLAST137

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 170Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105DUK. Bacteria.
COG0289. LUCA.
HOGENOMiHOG000227153.
KOiK00215.
OMAiYAREGHT.
OrthoDBiPOG091H01P6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB. 1 hit.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR023940. DHDPR_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C7BJY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTDIRVAI VGAGGRMGRQ LIQAVHQLSG VVLGVALERS GSSLLGTDAG
60 70 80 90 100
ELAGIGHIGV TVCDDLKNVV DDFDVLIDFT RPEGTLAHLE ICRRHSKAIV
110 120 130 140 150
IGTTGFDDEG KQAIKDASAN IPIVFAANFS VGVNLVLKLL EKAAKVMGEY
160 170 180 190 200
TDIEIIEAHH RHKVDAPSGT ALAMGESIAH ALGRDLKECA VYAREGYTGE
210 220 230 240 250
RDPKSIGFAT IRAGDIVGEH TAMFADVGER VEITHKASSR MTFANGAVKA
260 270
ALWLNGKNSG LFTMKDVLNL DLI
Length:273
Mass (Da):28,957
Last modified:September 22, 2009 - v1
Checksum:i9C657FA42BE8EEB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM162591 Genomic DNA. Translation: CAQ82652.1.

Genome annotation databases

EnsemblBacteriaiCAQ82652; CAQ82652; PAU_00560.
KEGGipay:PAU_00560.
PATRICi20494709. VBIPhoAsy71438_0644.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM162591 Genomic DNA. Translation: CAQ82652.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi553480.PAU_00560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ82652; CAQ82652; PAU_00560.
KEGGipay:PAU_00560.
PATRICi20494709. VBIPhoAsy71438_0644.

Phylogenomic databases

eggNOGiENOG4105DUK. Bacteria.
COG0289. LUCA.
HOGENOMiHOG000227153.
KOiK00215.
OMAiYAREGHT.
OrthoDBiPOG091H01P6.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.
BioCyciPASY291112:GJQ0-575-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB. 1 hit.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR023940. DHDPR_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC7BJY6_PHOAA
AccessioniPrimary (citable) accession number: C7BJY6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: November 30, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.