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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / NCIB 9290)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
Active sitei132 – 1321Proton acceptorUniRule annotation
Binding sitei159 – 1591Allosteric activator ADPUniRule annotation
Binding sitei167 – 1671Substrate; shared with dimeric partnerUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Binding sitei251 – 2511Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi106 – 1094ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHEP485917:GHL9-3329-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Phep_3293Imported
OrganismiPedobacter heparinus (strain ATCC 13125 / DSM 2366 / NCIB 9290)Imported
Taxonomic identifieri485917 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesSphingobacteriaceaePedobacter
ProteomesiUP000000852 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi485917.Phep_3293.

Structurei

3D structure databases

ProteinModelPortaliC6Y444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni130 – 1323Substrate bindingUniRule annotation
Regioni174 – 1763Substrate bindingUniRule annotation
Regioni190 – 1923Allosteric activator ADP bindingUniRule annotation
Regioni218 – 2203Allosteric activator ADP bindingUniRule annotation
Regioni257 – 2604Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiINALICI.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6Y444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPNIKNIAV LTSGGDAPGM NACIRAVVRT GIYNGINMFG VLQGYQGLIS
60 70 80 90 100
NNINPMDARS VSNIIHLGGT ILKTARCLEF KTDEGMALAY ENMKARDIDG
110 120 130 140 150
LVVIGGDGTF TGAKRFGEKF GIRVMGVPGT IDNDLYGSDF TLGYDTAINT
160 170 180 190 200
VIEAIDKIRD TADSHDRLFF IEVMGRDSGC IALRSAISSG AEAVLLPEKE
210 220 230 240 250
TSLNELISML ETGASTKKTS SIVIVSEGHK DGGAYEVAKK VKERFNHYDT
260 270 280 290 300
KVTILGHLQR GGSPSSFDRI LGSRLGFAAV NELLKGNTME MVGLRGNEVK
310 320
TTSIEEALTR HTFKLESDLL EMTKVLSI
Length:328
Mass (Da):35,364
Last modified:September 22, 2009 - v1
Checksum:i9227CB8DABFC3229
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001681 Genomic DNA. Translation: ACU05487.1.
RefSeqiWP_015809096.1. NZ_AQGK01000001.1.
YP_003093549.1. NC_013061.1.

Genome annotation databases

EnsemblBacteriaiACU05487; ACU05487; Phep_3293.
KEGGiphe:Phep_3293.
PATRICi22883743. VBIPedHep98714_3336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001681 Genomic DNA. Translation: ACU05487.1.
RefSeqiWP_015809096.1. NZ_AQGK01000001.1.
YP_003093549.1. NC_013061.1.

3D structure databases

ProteinModelPortaliC6Y444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi485917.Phep_3293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU05487; ACU05487; Phep_3293.
KEGGiphe:Phep_3293.
PATRICi22883743. VBIPedHep98714_3336.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiINALICI.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciPHEP485917:GHL9-3329-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13125 / DSM 2366 / NCIB 9290Imported.

Entry informationi

Entry nameiC6Y444_PEDHD
AccessioniPrimary (citable) accession number: C6Y444
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: April 29, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.