C6X8G4 (C6X8G4_METSD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182
Ordered Locus Names:	Msip34_0185 EMBL ACT49434.1

Organism

Methylovorus sp. (strain SIP3-4) (Methylotenera sp. (strain SIP3-4)) [Complete proteome] [HAMAP] EMBL ACT49434.1

Taxonomic identifier

582744 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylovorus › Methylovorus glucosotrophus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS005794
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS005794
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS005794
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS005794 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	C6X8G4 [UniParc]. Last modified September 22, 2009. Version 1. Checksum: 125F84B8D6C2E2D8	FASTA	316	33,407
	10 20 30 40 50 60 MPALKIIFAG TPDFAVPALA ALVASQHEVV MVLTQPDRPA GRGMKLKASP VKELALQHQI 70 80 90 100 110 120 PVLQPETLKD ADIQAQIAAV EADVMIVAAY GLIIPTSVLN MPALGCYNIH ASLLPRWRGA 130 140 150 160 170 180 APIQRALLAG DAETGVTIME VVPALDAGAM VEKGVLPITE RDTAQSLHDG LSAMGAQLMV 190 200 210 220 230 240 KAMDTLATQR SLPAEPQDEA LVTYAAKLQK AEAGIDWTQS AEAISRQVRA FNPFPVAQAK 250 260 270 280 290 300 LNEDICRIWM ATAMDGNAEP GVVVSVGDAI VVGCGQGLLR IEELQMPGGK RLQAAQFMAG 310 HALKPGDRFH SVLPQA

References

[1]

"Complete sequence of chromosome of Methylovorus sp. SIP3-4." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Kayluzhnaya M., Chistoserdova L. Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SIP3-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001674 Genomic DNA. Translation: ACT49434.1.
RefSeq	YP_003049961.1. NC_012969.1.
3D structure databases
ProteinModelPortal	C6X8G4.
ModBase	Search...
Protein-protein interaction databases
STRING	582744.Msip34_0185.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACT49434; ACT49434; Msip34_0185.
GeneID	8173463.
KEGG	mei:Msip34_0185.
PATRIC	22615338. VBIMetSp110381_0184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	CLSK2529103.
Enzyme and pathway databases
BioCyc	MGLU582744:GHXQ-194-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C6X8G4_METSD

Accession

Primary (citable) accession number: C6X8G4

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	May 29, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)