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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Methylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi250Magnesium or manganese 1UniRule annotation1
Metal bindingi263Magnesium or manganese 1UniRule annotation1
Metal bindingi263Magnesium or manganese 2UniRule annotation1
Metal bindingi265Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Mmol_2001Imported
OrganismiMethylotenera mobilis (strain JLW8 / ATCC BAA-1282 / DSM 17540)Imported
Taxonomic identifieri583345 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylotenera
Proteomesi
  • UP000002742 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi583345.Mmol_2001.

Structurei

3D structure databases

ProteinModelPortaliC6WYI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 296ATP-graspInterPro annotationAdd BLAST196

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
HOGENOMiHOG000011592.
KOiK01921.
OMAiYETKYTE.
OrthoDBiPOG091H00GT.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 2 hits.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

C6WYI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDFGKVAVL LGGKSGEREV SLKSGTAVLA ALQRQGVDAH AFDPATRPLH
60 70 80 90 100
DLESFDTAMI SLHGRFGEDG AMQGALELLG IPYTGSGVMA SAIGMDKWRT
110 120 130 140 150
KMMWSAAGVS TPAFELVSAD SDFEAIEQRL GLPLFVKPAN EGSSIGISKV
160 170 180 190 200
KQTGGLKAAY ELAAKSDPLV IAEQFVGGGE YTVGILGDQA LPIVRIVPKN
210 220 230 240 250
EFYDFEAKYL RDDTEYRCPC GLSAEKEAQI QQEALLAFNT IGCQGWGRVD
260 270 280 290 300
FLMDEAGKHY FLEVNTSPGM TDHSLVPMAA KAAGISFDEL VVSVLRLAHV

G
Length:301
Mass (Da):32,179
Last modified:September 22, 2009 - v1
Checksum:i81B34D91D7D9FA4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001672 Genomic DNA. Translation: ACT48903.1.
RefSeqiWP_015832938.1. NC_012968.1.

Genome annotation databases

EnsemblBacteriaiACT48903; ACT48903; Mmol_2001.
KEGGimmb:Mmol_2001.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiC6WYI4_METML
AccessioniPrimary (citable) accession number: C6WYI4
Entry historyiIntegrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: June 7, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported