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Protein

Glycine--tRNA ligase

Gene

glyQS

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glycine to tRNA(Gly).UniRule annotationSAAS annotation

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991SubstrateUniRule annotation
Binding sitei163 – 1631SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi195 – 1973ATPUniRule annotation
Nucleotide bindingi205 – 2106ATPUniRule annotation
Nucleotide bindingi282 – 2832ATPUniRule annotation
Nucleotide bindingi326 – 3294ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glycine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciAMIR446462:GH7R-1300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.14UniRule annotationSAAS annotation)
Alternative name(s):
Glycyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:glyQSUniRule annotation
Ordered Locus Names:Amir_1266Imported
OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)Imported
Taxonomic identifieri446462 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeActinosynnema
ProteomesiUP000002213: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi446462.Amir_1266.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 2145Substrate bindingUniRule annotation
Regioni322 – 3265Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0423.
HOGENOMiHOG000242016.
KOiK01880.
OMAiHKRFRAD.
OrthoDBiEOG6R87F8.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00253_B. Gly_tRNA_synth_B.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR022961. Gly_tRNA_ligase_bac.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6WRG6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVDRIETVV SLCKRRGFVY PCGEIYGGTR SAWDYGPLGV ELKDNIKRQW
60 70 80 90 100
WNFMVRGRED VVGLDSSVIL PREVWTASGH VEAFVDPLVE CNSCHKRFRQ
110 120 130 140 150
DTLSEEYAER TGKDVAEGDV SDVPCPNCGT RGQYTEPRMF NGLLKTHLGP
160 170 180 190 200
VETEEGLHYL RPETAQGIFT NFLNVQTTSR RKPPFGIGQI GKSFRNEITP
210 220 230 240 250
GNFIFRTREF EQMEMEFFVE PGTDEQWHQY WIDERTRWYT ELGISADNLR
260 270 280 290 300
HYEHPAEKLS HYAKRTVDIE YRFRFGGQEW GELEGIANRT DFDLTTHSNH
310 320 330 340 350
SGVDLSYFDQ ATNSRYRPFV IEPAAGVGRP MMAFLLEAYT EDEAPNAKGG
360 370 380 390 400
VDKRVVLRLD RRLAPVKAAV LPLSRNADLS PKAKDLAAAL RKHWNVEFDD
410 420 430 440 450
AGAIGRRYRR QDEIGTPFCV TVDFDTLTDH AVTVRERDTM SQERVSMDQI
460
EGYLAARLIG A
Length:461
Mass (Da):52,682
Last modified:September 22, 2009 - v1
Checksum:i7049E38ECC4E0AD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA. Translation: ACU35218.1.
RefSeqiYP_003099064.1. NC_013093.1.

Genome annotation databases

EnsemblBacteriaiACU35218; ACU35218; Amir_1266.
GeneIDi8325443.
KEGGiami:Amir_1266.
PATRICi20765347. VBIActMir80567_1290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA. Translation: ACU35218.1.
RefSeqiYP_003099064.1. NC_013093.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi446462.Amir_1266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU35218; ACU35218; Amir_1266.
GeneIDi8325443.
KEGGiami:Amir_1266.
PATRICi20765347. VBIActMir80567_1290.

Phylogenomic databases

eggNOGiCOG0423.
HOGENOMiHOG000242016.
KOiK01880.
OMAiHKRFRAD.
OrthoDBiEOG6R87F8.

Enzyme and pathway databases

BioCyciAMIR446462:GH7R-1300-MONOMER.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00253_B. Gly_tRNA_synth_B.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR022961. Gly_tRNA_ligase_bac.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971Imported.

Entry informationi

Entry nameiC6WRG6_ACTMD
AccessioniPrimary (citable) accession number: C6WRG6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.