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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101D-inositol 3-phosphateUniRule annotation1
Binding sitei24UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei791D-inositol 3-phosphateUniRule annotation1
Binding sitei1121D-inositol 3-phosphateUniRule annotation1
Binding sitei1361D-inositol 3-phosphateUniRule annotation1
Binding sitei1561D-inositol 3-phosphateUniRule annotation1
Binding sitei230UDP-GlcNAcUniRule annotation1
Binding sitei235UDP-GlcNAcUniRule annotation1
Binding sitei296UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi305Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi306Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi308Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei318UDP-GlcNAcUniRule annotation1
Binding sitei326UDP-GlcNAcUniRule annotation1
Metal bindingi332MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Amir_6757
OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Taxonomic identifieri446462 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeActinosynnema
Proteomesi
  • UP000002213 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001081 – 417D-inositol 3-phosphate glycosyltransferaseAdd BLAST417

Proteomic databases

PRIDEiC6WPK3.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi446462.Amir_6757.

Structurei

3D structure databases

ProteinModelPortaliC6WPK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 17UDP-GlcNAc bindingUniRule annotation2
Regioni21 – 261D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

C6WPK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVAVLSVH TSPLEQPGTG DAGGMNVYVV QTATAMARRG VEVEIFTRAT
60 70 80 90 100
SSELPPVAEL APGVKVRHVV AGPFSGLEKE ELPGQLCAFT AGVLRAEARH
110 120 130 140 150
EPGHYDVVHS HYWLSGQVGW LARERWGVPL VHTAHTLAKV KNLALADSDA
160 170 180 190 200
PEPRMRVIGE EQVVAEADRL IANTDVEADQ LTGLYAADPA KVLVVPPGVD
210 220 230 240 250
LGRFTPGDRG EARRSLGLAP DALVLAFVGR IQPLKAPDVL VRATAALLER
260 270 280 290 300
DPGLRSRLVV LVVGGPSGSG MRTPDELVGL ARSLGVADVV RFLPPQGGGS
310 320 330 340 350
LAQVYRAADA VAVPSHNESF GLVALEAQAC GTPVVAAAVG GLPVAVRDGV
360 370 380 390 400
TGLLVAGHRT SDWADALSRI ALAPGLREAL AGNAVGHARG FSWDRTTESL
410
LAAYGQARDV FREEVYA
Length:417
Mass (Da):43,718
Last modified:September 22, 2009 - v1
Checksum:iB57A776243126E16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA. Translation: ACU40554.1.
RefSeqiWP_015805431.1. NC_013093.1.

Genome annotation databases

EnsemblBacteriaiACU40554; ACU40554; Amir_6757.
KEGGiami:Amir_6757.
PATRICi20776753. VBIActMir80567_6980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA. Translation: ACU40554.1.
RefSeqiWP_015805431.1. NC_013093.1.

3D structure databases

ProteinModelPortaliC6WPK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi446462.Amir_6757.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEiC6WPK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU40554; ACU40554; Amir_6757.
KEGGiami:Amir_6757.
PATRICi20776753. VBIActMir80567_6980.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_ACTMD
AccessioniPrimary (citable) accession number: C6WPK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.