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C6WKM8

- C6WKM8_ACTMD

UniProt

C6WKM8 - C6WKM8_ACTMD

Protein

Isocitrate dehydrogenase [NADP]

Gene

Amir_6478

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateUniRule annotation
    Binding sitei82 – 821NADP
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Sitei139 – 1391Critical for catalysisUniRule annotation
    Sitei212 – 2121Critical for catalysisUniRule annotation
    Metal bindingi252 – 2521Magnesium or manganeseUniRule annotation
    Binding sitei260 – 2601NADP
    Metal bindingi275 – 2751Magnesium or manganeseUniRule annotation
    Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygen

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADP
    Nucleotide bindingi310 – 3156NADP

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Enzyme and pathway databases

    BioCyciAMIR446462:GH7R-6544-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    Ordered Locus Names:Amir_6478Imported
    OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)Imported
    Taxonomic identifieri446462 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeActinosynnema
    ProteomesiUP000002213: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi446462.Amir_6478.

    Structurei

    3D structure databases

    ProteinModelPortaliC6WKM8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0538.
    HOGENOMiHOG000019858.
    KOiK00031.
    OMAiFKDEFQR.
    OrthoDBiEOG628F3X.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6WKM8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKIKVQGTV VELDGDEMTR IIWQFIKDKL VHPYLDVNLE YYDLGIEHRD    50
    ATDDQVTIDA ANAIKKHNVG VKCATITPDE ARVEEFGLKK MWVSPNGTIR 100
    NILGGVVFRE PIIISNIPRL VPGWTKPIII GRHAHGDQYK ATNFKVPGAG 150
    KLTVTFTPED GSAPIEHVVA NYGADGGVAM GMYNFNKSIE DFARASFSYG 200
    LQRNYPVYMS TKNTILKAYD GAFKDIFQQV FEAEFKAEFD AKGLTYEHRL 250
    IDDMVAAAMK WEGGYVWACK NYDGDVQSDT VAQGFGSLGL MTSVLMTPDG 300
    KTVEAEAAHG TVTRHFRQHQ AGKPTSTNPI ASIFAWTGGL KHRGKLDGTP 350
    EVTGFAETLE KVIIETVESG KMTKDLALLV GRDQEWQTTE DFLATLDENL 400
    QKKMAG 406
    Length:406
    Mass (Da):44,879
    Last modified:September 22, 2009 - v1
    Checksum:iB0E3E0D3998AFE7A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001630 Genomic DNA. Translation: ACU40279.1.
    RefSeqiWP_015805162.1. NC_013093.1.
    YP_003104125.1. NC_013093.1.

    Genome annotation databases

    EnsemblBacteriaiACU40279; ACU40279; Amir_6478.
    GeneIDi8330689.
    KEGGiami:Amir_6478.
    PATRICi20776163. VBIActMir80567_6689.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001630 Genomic DNA. Translation: ACU40279.1 .
    RefSeqi WP_015805162.1. NC_013093.1.
    YP_003104125.1. NC_013093.1.

    3D structure databases

    ProteinModelPortali C6WKM8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 446462.Amir_6478.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACU40279 ; ACU40279 ; Amir_6478 .
    GeneIDi 8330689.
    KEGGi ami:Amir_6478.
    PATRICi 20776163. VBIActMir80567_6689.

    Phylogenomic databases

    eggNOGi COG0538.
    HOGENOMi HOG000019858.
    KOi K00031.
    OMAi FKDEFQR.
    OrthoDBi EOG628F3X.

    Enzyme and pathway databases

    BioCyci AMIR446462:GH7R-6544-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971Imported.

    Entry informationi

    Entry nameiC6WKM8_ACTMD
    AccessioniPrimary (citable) accession number: C6WKM8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3