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C6WIE4

- PAFA_ACTMD

UniProt

C6WIE4 - PAFA_ACTMD

Protein

Pup--protein ligase

Gene

pafA

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.UniRule annotation

    Catalytic activityi

    ATP + [Pup] + [protein]-lysine = ADP + phosphate + [protein]-N-pupyllysine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91MagnesiumUniRule annotation
    Binding sitei53 – 531ATPUniRule annotation
    Metal bindingi55 – 551MagnesiumUniRule annotation
    Active sitei57 – 571Proton acceptorUniRule annotation
    Metal bindingi63 – 631MagnesiumUniRule annotation
    Binding sitei66 – 661ATP; via carbonyl oxygenUniRule annotation
    Binding sitei419 – 4191ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. small conjugating protein ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
    2. proteasomal protein catabolic process Source: UniProtKB-HAMAP
    3. protein pupylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAMIR446462:GH7R-2292-MONOMER.
    UniPathwayiUPA00997.
    UPA00998.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pup--protein ligaseUniRule annotation (EC:6.3.2.n2UniRule annotation)
    Alternative name(s):
    Proteasome accessory factor AUniRule annotation
    Pup-conjugating enzymeUniRule annotation
    Gene namesi
    Name:pafAUniRule annotation
    Ordered Locus Names:Amir_2247
    OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
    Taxonomic identifieri446462 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeActinosynnema
    ProteomesiUP000002213: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Pup--protein ligasePRO_0000395891Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi446462.Amir_2247.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiNOG04175.
    HOGENOMiHOG000264267.
    KOiK13571.
    OMAiHDKAGER.
    OrthoDBiEOG6PS5V6.

    Family and domain databases

    HAMAPiMF_02111. Pup_ligase.
    InterProiIPR022279. Pup_ligase.
    IPR004347. Pup_ligase/deamidase.
    [Graphical view]
    PfamiPF03136. Pup_ligase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018077. UCP018077. 1 hit.
    TIGRFAMsiTIGR03686. pupylate_PafA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C6WIE4-1 [UniParc]FASTAAdd to Basket

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    MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLRN    50
    GSRLYLDVGS HPEYATAECD DLAQLVSHDK AGERILEDLL VDAERRLADE 100
    GIGGDIFLFK NNTDSAGNSY GCHENYLVAR AGEFSRIADV LLPFLVTRQL 150
    ICGAGKVLQT PRGAVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA 200
    ERYRRLHVIV GDSNMSEVTT LLKVGSANLV LEMIEQGVQF RDFSLDNPIR 250
    AIREISHDLT GRRTVKLAGG KEASALDIQR EYYERAVEHV AKRSPDPMAE 300
    RVLELWGRTL DAIAAQDLSK IDREIDWAIK HRLLERYQAK HDMDLSNPRI 350
    AQLDLAYHDI RRGRGVFDLL QRKGQVERVT DDGEIEAAKD TPPQTTRAKL 400
    RGDFIAAAQA AGRDFTVDWV HLKLNDQAQR TVLCKDPFRA VDERVERLIA 450
    SL 452
    Length:452
    Mass (Da):51,144
    Last modified:September 22, 2009 - v1
    Checksum:iB3D3D861D86BCC59
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001630 Genomic DNA. Translation: ACU36187.1.
    RefSeqiYP_003100033.1. NC_013093.1.

    Genome annotation databases

    EnsemblBacteriaiACU36187; ACU36187; Amir_2247.
    GeneIDi8326436.
    KEGGiami:Amir_2247.
    PATRICi20767401. VBIActMir80567_2309.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001630 Genomic DNA. Translation: ACU36187.1 .
    RefSeqi YP_003100033.1. NC_013093.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 446462.Amir_2247.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACU36187 ; ACU36187 ; Amir_2247 .
    GeneIDi 8326436.
    KEGGi ami:Amir_2247.
    PATRICi 20767401. VBIActMir80567_2309.

    Phylogenomic databases

    eggNOGi NOG04175.
    HOGENOMi HOG000264267.
    KOi K13571.
    OMAi HDKAGER.
    OrthoDBi EOG6PS5V6.

    Enzyme and pathway databases

    UniPathwayi UPA00997 .
    UPA00998 .
    BioCyci AMIR446462:GH7R-2292-MONOMER.

    Family and domain databases

    HAMAPi MF_02111. Pup_ligase.
    InterProi IPR022279. Pup_ligase.
    IPR004347. Pup_ligase/deamidase.
    [Graphical view ]
    Pfami PF03136. Pup_ligase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018077. UCP018077. 1 hit.
    TIGRFAMsi TIGR03686. pupylate_PafA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971.

    Entry informationi

    Entry nameiPAFA_ACTMD
    AccessioniPrimary (citable) accession number: C6WIE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3