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C6WID8 (PSB_ACTMD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta

EC=3.4.25.1
Alternative name(s):
20S proteasome beta subunit
Proteasome core protein PrcB
Gene names
Name:prcB
Ordered Locus Names:Amir_2241
OrganismActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971) [Complete proteome] [HAMAP]
Taxonomic identifier446462 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeActinosynnema

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation By similarity. HAMAP-Rule MF_02113

Catalytic activity

Cleavage of peptide bonds with very broad specificity. HAMAP-Rule MF_02113

Enzyme regulation

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. HAMAP-Rule MF_02113

Pathway

Protein degradation; proteasomal Pup-dependent pathway. HAMAP-Rule MF_02113

Subunit structure

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02113.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 5555Removed in mature form; by autocatalysis By similarity
PRO_0000397492
Chain56 – 282227Proteasome subunit beta HAMAP-Rule MF_02113
PRO_0000397493

Sites

Active site561Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
C6WID8 [UniParc].

Last modified September 22, 2009. Version 1.
Checksum: F0664FC22C3F51D4

FASTA28229,490
        10         20         30         40         50         60 
MDNSSTGRYP AASLPPAYLR PGSSSFTDFL RAQAPELLPT ARSFPEGSVV QAAHGTTIVA 

        70         80         90        100        110        120 
LTFKGGVVIA GDRRATMGNV IAQRDMKKVF VTDDYSAVGI AGTAGIAIEI VRLFAVELRH 

       130        140        150        160        170        180 
YEKIEGVSLS LDGKANRLSG MVKGNLDAAL AGLAVVPLFV GYDTDAADPD RAGRIVSYDV 

       190        200        210        220        230        240 
TGARFEETLG YQSVGSGSLF AKSALKKLYD PDADAEGAVR AAVEALYDAA DDDSATGGPD 

       250        260        270        280 
LVRRIFPVVV TVTAEGAVHL PEERTSAIAE TVVEGRRARP AG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001630 Genomic DNA. Translation: ACU36181.1.
RefSeqYP_003100027.1. NC_013093.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING446462.Amir_2241.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACU36181; ACU36181; Amir_2241.
GeneID8326430.
KEGGami:Amir_2241.
PATRIC20767383. VBIActMir80567_2300.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000245308.
KOK03433.
OMAFQVELEH.
OrthoDBEOG6XM79W.

Enzyme and pathway databases

BioCycAMIR446462:GH7R-2286-MONOMER.
UniPathwayUPA00997.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
HAMAPMF_02113_B. Proteasome_B_B.
InterProIPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR03690. 20S_bact_beta. 1 hit.
PROSITEPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSB_ACTMD
AccessionPrimary (citable) accession number: C6WID8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: September 22, 2009
Last modified: June 11, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways