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C6WID8

- PSB_ACTMD

UniProt

C6WID8 - PSB_ACTMD

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Protein

Proteasome subunit beta

Gene

prcB

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561NucleophileUniRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
  2. proteasomal protein catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciAMIR446462:GH7R-2286-MONOMER.
UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PrcBUniRule annotation
Gene namesi
Name:prcBUniRule annotation
Ordered Locus Names:Amir_2241
OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Taxonomic identifieri446462 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeActinosynnema
ProteomesiUP000002213: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 5555Removed in mature form; by autocatalysisUniRule annotationPRO_0000397492Add
BLAST
Chaini56 – 282227Proteasome subunit betaPRO_0000397493Add
BLAST

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

Protein-protein interaction databases

STRINGi446462.Amir_2241.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000245308.
KOiK03433.
OMAiFQVELEH.
OrthoDBiEOG6XM79W.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_B. Proteasome_B_B.
InterProiIPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C6WID8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNSSTGRYP AASLPPAYLR PGSSSFTDFL RAQAPELLPT ARSFPEGSVV
60 70 80 90 100
QAAHGTTIVA LTFKGGVVIA GDRRATMGNV IAQRDMKKVF VTDDYSAVGI
110 120 130 140 150
AGTAGIAIEI VRLFAVELRH YEKIEGVSLS LDGKANRLSG MVKGNLDAAL
160 170 180 190 200
AGLAVVPLFV GYDTDAADPD RAGRIVSYDV TGARFEETLG YQSVGSGSLF
210 220 230 240 250
AKSALKKLYD PDADAEGAVR AAVEALYDAA DDDSATGGPD LVRRIFPVVV
260 270 280
TVTAEGAVHL PEERTSAIAE TVVEGRRARP AG
Length:282
Mass (Da):29,490
Last modified:September 22, 2009 - v1
Checksum:iF0664FC22C3F51D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001630 Genomic DNA. Translation: ACU36181.1.
RefSeqiYP_003100027.1. NC_013093.1.

Genome annotation databases

EnsemblBacteriaiACU36181; ACU36181; Amir_2241.
GeneIDi8326430.
KEGGiami:Amir_2241.
PATRICi20767383. VBIActMir80567_2300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001630 Genomic DNA. Translation: ACU36181.1 .
RefSeqi YP_003100027.1. NC_013093.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 446462.Amir_2241.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACU36181 ; ACU36181 ; Amir_2241 .
GeneIDi 8326430.
KEGGi ami:Amir_2241.
PATRICi 20767383. VBIActMir80567_2300.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000245308.
KOi K03433.
OMAi FQVELEH.
OrthoDBi EOG6XM79W.

Enzyme and pathway databases

UniPathwayi UPA00997 .
BioCyci AMIR446462:GH7R-2286-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_02113_B. Proteasome_B_B.
InterProi IPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03690. 20S_bact_beta. 1 hit.
PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971.

Entry informationi

Entry nameiPSB_ACTMD
AccessioniPrimary (citable) accession number: C6WID8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: September 22, 2009
Last modified: October 29, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3