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Protein

Kynureninase

Gene

kynU

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.UniRule annotation

Catalytic activityi

L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation
L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Pyridoxal phosphate; via amide nitrogenUniRule annotation
Binding sitei86 – 861Pyridoxal phosphateUniRule annotation
Binding sitei179 – 1791Pyridoxal phosphateUniRule annotation
Binding sitei182 – 1821Pyridoxal phosphateUniRule annotation
Binding sitei204 – 2041Pyridoxal phosphateUniRule annotation
Binding sitei234 – 2341Pyridoxal phosphateUniRule annotation
Binding sitei260 – 2601Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. kynureninase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. L-kynurenine catabolic process Source: UniProtKB-UniPathway
  4. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  5. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Pyridine nucleotide biosynthesisUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

BioCyciAMIR446462:GH7R-279-MONOMER.
UniPathwayiUPA00253; UER00329.

Names & Taxonomyi

Protein namesi
Recommended name:
KynureninaseUniRule annotation (EC:3.7.1.3UniRule annotation)
Alternative name(s):
L-kynurenine hydrolaseUniRule annotation
Gene namesi
Name:kynUUniRule annotation
Ordered Locus Names:Amir_0265Imported
OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)Imported
Taxonomic identifieri446462 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeActinosynnema
ProteomesiUP000002213: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei205 – 2051N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi446462.Amir_0265.

Structurei

3D structure databases

ProteinModelPortaliC6WFA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni112 – 1154Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the kynureninase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3844.
HOGENOMiHOG000242437.
KOiK01556.
OMAiRFWQPLS.
OrthoDBiEOG6N67XP.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.

Sequencei

Sequence statusi: Complete.

C6WFA2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDPLAHVRE LFDLADDVVY LDGNSLGAPP RAVAERLDDV VRRQWGRRLI
60 70 80 90 100
RSWSEGWWEA PQRVGDRIGR LVGAAPGQVV VGDSTSVNLF KALVGAVRGT
110 120 130 140 150
DRSEIVVDAG TFPTDGYIAG SAAELTGLSL RPADPDDLRL DERTAVVLLN
160 170 180 190 200
HVDYRTGRLL DMAGLTERAH EVGAKVVWDL CHSVGVLPIE LDALGVDLAV
210 220 230 240 250
GCTYKFLNGG PGSPAFLYVP SAAQPGFRQP LTGWTGHREP FAMAAEYTPD
260 270 280 290 300
AGIGRARAGT PDILSMLALD AALDVWDGVE LADVRAKGLA LTARFMELVR
310 320 330 340 350
DLPGIEVITP ADGRRGHQVS LRHPEAARLM AELIAGGVIG DHRPPDVLRF
360 370
GFAPLYNTFD EAERAAKALE ELL
Length:373
Mass (Da):40,159
Last modified:September 22, 2009 - v1
Checksum:iFAB9E82801C19316
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA. Translation: ACU34234.1.
RefSeqiYP_003098080.1. NC_013093.1.

Genome annotation databases

EnsemblBacteriaiACU34234; ACU34234; Amir_0265.
GeneIDi8324422.
KEGGiami:Amir_0265.
PATRICi20763285. VBIActMir80567_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA. Translation: ACU34234.1.
RefSeqiYP_003098080.1. NC_013093.1.

3D structure databases

ProteinModelPortaliC6WFA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi446462.Amir_0265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU34234; ACU34234; Amir_0265.
GeneIDi8324422.
KEGGiami:Amir_0265.
PATRICi20763285. VBIActMir80567_0270.

Phylogenomic databases

eggNOGiCOG3844.
HOGENOMiHOG000242437.
KOiK01556.
OMAiRFWQPLS.
OrthoDBiEOG6N67XP.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
BioCyciAMIR446462:GH7R-279-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971Imported.

Entry informationi

Entry nameiC6WFA2_ACTMD
AccessioniPrimary (citable) accession number: C6WFA2
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: February 4, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.