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C6W7X3 (C6W7X3_ACTMD) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172
Ordered Locus Names:Amir_3078
OrganismActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971) [Complete proteome] [HAMAP]
Taxonomic identifier446462 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Actinosynnemataceae › Actinosynnema

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6291Zinc By similarity HAMAP MF_00172
Metal binding6311Zinc By similarity HAMAP MF_00172
Metal binding7141Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
C6W7X3 [UniParc].

Last modified September 22, 2009. Version 1.
Checksum: 2C712B74DA43D3E1

FASTA74480,975
        10         20         30         40         50         60 
MTSGIGSTVL GYPRIGPRRE LKRAVESYWA GRTDRDALLA TAARLRADTW RDLREAGLDS 

        70         80         90        100        110        120 
VPSNTFSFYD QVLDTAALVG AIPARYRQDH EVDTLFAAAR GTGSLAPLEL TKWFDTNYHY 

       130        140        150        160        170        180 
LVPEIDETTT FALTGDKPLA EHREARAQGV PTRPVVLGPV TFLLLAKGVD PLSRLDELVE 

       190        200        210        220        230        240 
VYADLLRGLA DEGVEWVQLD EPAFAADRSE RELVALERAY TRLAAQSARP QLLVTGYYGD 

       250        260        270        280        290        300 
LGRALGVLAG TAVDALAVDL VAGSEEGLAE VTALRGKILV AGLVDGRNVW RTDLRAALAR 

       310        320        330        340        350        360 
AEALRPLARE VVVSTSSTLL HVPYDLDQEQ GLGEEVRDRL AFARQKVHEV VLLGRALAEG 

       370        380        390        400        410        420 
PTEEWEAEAD RPRPAPRHDG AVRSRLTGLQ PDARTRAPYA DRVAAQSDLG LPRLATTTIG 

       430        440        450        460        470        480 
SFPQTTEIRT ARAALRSGEL DLDGYAERMR TEIGRVIALQ EDIGLDVLVH GEPERNDMVQ 

       490        500        510        520        530        540 
YFAEHLDGVA GTELGWVQSY GSRCVRPPIL HGDVTRPEPI TVPWTTHARS LTTRQVKGML 

       550        560        570        580        590        600 
TGPVTILAWS FVRDDQPLRA TADQVALALR DEIADLEAAG TRVIQVDEPA LRELLPLREA 

       610        620        630        640        650        660 
DREDYLDWSV SAFRLATGGA AAGTQVHTHL CYSEFGEVIE AIDALDADVT TIEAARSKME 

       670        680        690        700        710        720 
VLADLSAAGF ARGVGPGVYD IHSPRVPGEE ELVALIAEAS RAVPGDRLWV NPDCGLKTRA 

       730        740 
YAEVEPALRA LVAAAKRVRE TTTN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001630 Genomic DNA. Translation: ACU36994.1.
RefSeqYP_003100840.1. NC_013093.1.

3D structure databases

ProteinModelPortalC6W7X3.
ModBaseSearch...

Protein-protein interaction databases

STRINGC6W7X3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8327268.
GenomeReviewsGene locus Amir_3078 in contig CP001630_GR.
KEGGami:Amir_3078.
PATRIC20769120. VBIActMir80567_3170.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMARNIWRAN.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC6W7X3_ACTMD
AccessionPrimary (citable) accession number: C6W7X3
Entry history
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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