C6VKY4 (C6VKY4_LACPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193 EMBL ACT61352.1
Ordered Locus Names:	JDM1_0463

Organism

Lactobacillus plantarum (strain JDM1) [Complete proteome] [HAMAP]

Taxonomic identifier

644042 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	49	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	C6VKY4 [UniParc]. Last modified September 22, 2009. Version 1. Checksum: A1C78763ED7949D5	FASTA	275	30,590
	10 20 30 40 50 60 MRPLQAEIIK ALHVAPTIDP EVEIRRSIDF LKAYLTKNTF LKTYVLGISG GQDSTLAGKL 70 80 90 100 110 120 TEMAITEMRQ ETGDDRYQFI AVRLPYGNQA DEADAMAAID FMQADVTDRV DIQPATDAMV 130 140 150 160 170 180 TALEANQLTI HDFNKGNIKA RQRMIVQYGI AGEMHGAVVG TDHAAEAVTG FYTKYGDGGA 190 200 210 220 230 240 DIVPLWRLNK RQGKQMLAAL DAPKHLYDKV PTADLEEDRP ALPDEVALGV RYDDIDDYLE 250 260 270 GRTVSDAAAE KIEAWYLKTA HKRHAAITVF DDFWK

References

[1]	"Complete genome sequence of Lactobacillus plantarum JDM1." Zhang Z.-Y., Liu C., Zhu Y.-Z., Zhong Y., Zhu Y.-Q., Zheng H.-J., Zhao G.-P., Wang S.-Y., Guo X.-K. J. Bacteriol. 191:5020-5021(2009) [PubMed: 19465650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001617 Genomic DNA. Translation: ACT61352.1.
RefSeq	YP_003062049.1. NC_012984.1.
3D structure databases
ProteinModelPortal	C6VKY4.
SMR	C6VKY4. Positions 4-275.
ModBase	Search...
Protein-protein interaction databases
STRING	C6VKY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8189344.
GenomeReviews	Gene locus JDM1_0463 in contig CP001617_GR.
KEGG	lpj:JDM1_0463.
PATRIC	22241447. VBILacPla68346_0458.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C6VKY4_LACPJ

Accession

Primary (citable) accession number: C6VKY4

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)