Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Neorickettsia risticii (strain Illinois)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotationSAAS annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotationSAAS annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi266 – 2661Manganese 2UniRule annotation
Metal bindingi271 – 2711Manganese 1UniRule annotation
Metal bindingi271 – 2711Manganese 2UniRule annotation
Active sitei278 – 2781UniRule annotation
Metal bindingi289 – 2891Manganese 2UniRule annotation
Metal bindingi348 – 3481Manganese 1UniRule annotation
Metal bindingi350 – 3501Manganese 1UniRule annotation
Metal bindingi350 – 3501Manganese 2UniRule annotation
Active sitei352 – 3521UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

AminopeptidaseUniRule annotationSAAS annotation, Hydrolase, Protease

Keywords - Ligandi

ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciNRIS434131:GJCC-452-MONOMER.

Protein family/group databases

MEROPSiM17.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation
Alternative name(s):
Leucine aminopeptidaseUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:NRI_0450Imported
OrganismiNeorickettsia risticii (strain Illinois)Imported
Taxonomic identifieri434131 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeNeorickettsia
ProteomesiUP000001627: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi434131.NRI_0450.

Structurei

3D structure databases

ProteinModelPortaliC6V4W6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243132.
KOiK01255.
OMAiAMELGFP.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6V4W6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVFFLSPVE AMQSEPILFS GLYENSEFFG RISILDQQSS SFISKSIAST
60 70 80 90 100
SFTGKIGENV CIFLSDLVEG YPKLQQLCLI GLGKQKDFGA HTAEKIGTQI
110 120 130 140 150
ASLMKKNNVS SATVLLDGLK DDYALDLAFG AKVKDYSFNK YKTKKVDDKS
160 170 180 190 200
ITLERLVIGI ENYEHICSVF REKVEPLIES IKLTRDLINE PANHLNPETY
210 220 230 240 250
ANAVREITKT APNLKIEILD EKIMQKLGMN ALLGVGQGSA YPSRLAVVKY
260 270 280 290 300
NGAVDKDEPY IALVGKGVTF DSGGISLKPA RGMWHMISDM AGSATVLGAI
310 320 330 340 350
HAVAKKGVKA NVAAVLGIVE NAVSGVAQRP GDVVKSASGK TIEVLNTDAE
360 370 380 390 400
GRLVLADALW YAQEHLKANQ VIDVATLTGA IVVALGHDHA GLFSNDDVLA
410 420 430 440 450
ENLANIGKKV GEKLWRMPMS KNYDDLVNSE VADVKNISTE NHGADSITAA
460 470 480 490 500
QFLKRFINDG TKWAHLDIAG VAWNNSTSHF SSTGASGFGV RLLTEFISES

AKDTSK
Length:506
Mass (Da):54,634
Last modified:September 22, 2009 - v1
Checksum:iCFFECDAD256EBCAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001431 Genomic DNA. Translation: ACT69436.1.
RefSeqiWP_015816323.1. NC_013009.1.
YP_003081670.1. NC_013009.1.

Genome annotation databases

EnsemblBacteriaiACT69436; ACT69436; NRI_0450.
GeneIDi8220514.
KEGGinri:NRI_0450.
PATRICi22679184. VBINeoRis104330_0426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001431 Genomic DNA. Translation: ACT69436.1.
RefSeqiWP_015816323.1. NC_013009.1.
YP_003081670.1. NC_013009.1.

3D structure databases

ProteinModelPortaliC6V4W6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi434131.NRI_0450.

Protein family/group databases

MEROPSiM17.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACT69436; ACT69436; NRI_0450.
GeneIDi8220514.
KEGGinri:NRI_0450.
PATRICi22679184. VBINeoRis104330_0426.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243132.
KOiK01255.
OMAiAMELGFP.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciNRIS434131:GJCC-452-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever."
    Lin M., Zhang C., Gibson K., Rikihisa Y.
    Nucleic Acids Res. 37:6076-6091(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IllinoisImported.

Entry informationi

Entry nameiC6V4W6_NEORI
AccessioniPrimary (citable) accession number: C6V4W6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: March 4, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.