Periplasmic nitrate reductase - Escherichia coli O157:H7 (strain TW14359 / EHEC)

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C6V2U2 (C6V2U2_ECO5T) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 32. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase HAMAP-Rule MF_01630
EC=1.7.99.4 HAMAP-Rule MF_01630

Gene names

Name:	napA HAMAP-Rule MF_01630 EMBL ACT72866.1
Ordered Locus Names:	ECSP_3085 EMBL ACT72866.1

Organism

Escherichia coli O157:H7 (strain TW14359 / EHEC) [Complete proteome] [HAMAP] EMBL ACT72866.1

Taxonomic identifier

544404 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630 SAAS SAAS006656
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 1 molybdenum ion per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subunit structure	Interacts with NapB By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subcellular location	Periplasm By similarity HAMAP-Rule MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven By similarity. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. HAMAP-Rule MF_01630 Contains 1 4Fe-4S Mo/W bis-MGD-type domain. HAMAP-Rule MF_01630

Ontologies

Keywords
Biological process	Electron transport HAMAP-Rule MF_01630 SAAS SAAS006656 Nitrate assimilation HAMAP-Rule MF_01630 SAAS SAAS006656 Transport
Cellular component	Periplasm HAMAP-Rule MF_01630
Domain	Signal HAMAP-Rule MF_01630
Ligand	4Fe-4S HAMAP-Rule MF_01630 SAAS SAAS006656 Iron Iron-sulfur Metal-binding Molybdenum HAMAP-Rule MF_01630 SAAS SAAS006656
Molecular function	Oxidoreductase HAMAP-Rule MF_01630 SAAS SAAS006656
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

Tat-type signal By similarity HAMAP-Rule MF_01630

Regions

Domain

39 – 95

4Fe-4S Mo/W bis-MGD-type By similarity HAMAP-Rule MF_01630

Sites

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Sequences

Sequence

Length

Mass (Da)

Tools

C6V2U2 [UniParc].

Last modified September 22, 2009. Version 1.
Checksum: 967121CB05C59323
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FASTA

828

93,057

        10         20         30         40         50         60 
MKLSRRSFMK ANAVAAAAAA AGLSVPGVAR AVVGQQEAIK WDKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
QGRVVACQGD PDAPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKNGKYD KEGEFTPITW 

       130        140        150        160        170        180 
DQAFDVMEEK FKTALKEKGP ESIGMFGSGQ WTIWEGYAAS KLFKAGFRSN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGANMAEMH PILWSRITNR RLSNQDVTVA 

       250        260        270        280        290        300 
VLSTYQHRSF ELADNGIIFT PQSDLVILNY IANYIIQNNA INQDFFSKHV NLRKGATDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFEDYK AFVAEYTLEK TAEMTGVPKD QLEQLAQLYA 

       370        380        390        400        410        420 
DPNKKVISYW TMGFNQHTRG VWANNLVYNL HLLTGKISQP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD MVVTNEKHRD ICEKKWNIPS GTIPAKIGLH AVAQDRALKD GKLNVYWTMC 

       490        500        510        520        530        540 
TNNMQAGPNI NEERMPGWRD PRNFIIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVQ APGEAKSDLW QLVQFSRRFK TEEVWPEELL AKKPELRGKT LYEVLYATPE 

       610        620        630        640        650        660 
VSKFPVSELA EDQLNDESRE LGFYLQKGLF EEYAWFGRGH GHDLAPFDDY HKARGLRWPV 

       670        680        690        700        710        720 
VNGKETQWRY SEGNDPYVKA GEGYKFYGKP DGKAVIFALP FEPAAEAPDE EYDLWLSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARDLRR GDKVKVVSRR GEVISIVETR 

       790        800        810        820 
GRNRPPQGLV YMPFFDAAQL VNKLTLDATD PLSKETDFKK CAVKLEKV

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References

[1]

"Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhance virulence."
Kulasekara B.R., Jacobs M., Zhou Y., Wu Z., Sims E., Saenphimmachak C., Rohmer L., Ritchie J.M., Radey M., McKevitt M., Freeman T.L., Hayden H., Haugen E., Gillett W., Fong C., Chang J., Beskhlebnaya V., Waldor M.K.

Miller S.I.
Infect. Immun. 77:3713-3721(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TW14359 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001368 Genomic DNA. Translation: ACT72866.1.
RefSeq	YP_003078942.1. NC_013008.1.
3D structure databases
ProteinModelPortal	C6V2U2.
SMR	C6V2U2. Positions 1-35, 37-827.
ModBase	Search...
Protein-protein interaction databases
STRING	544404.ECSP_3085.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACT72866; ACT72866; ECSP_3085.
GeneID	8217651.
KEGG	etw:ECSP_3085.
PATRIC	18390220. VBIEscCol9396_3137.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	HWIKAAR.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	ECOL544404:GKCX-3081-MONOMER.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_4Fe-4S_dom. IPR027467. MopterinOxRdtase_cofactor_BS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6V2U2_ECO5T

Accession

Primary (citable) accession number: C6V2U2

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	May 29, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information