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C6V1K0 (C6V1K0_ECO5T) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
EC=2.7.1.50 HAMAP-Rule MF_00228
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase HAMAP-Rule MF_00228
Gene names
Name:thiM HAMAP-Rule MF_00228 EMBL ACT72688.1
Ordered Locus Names:ECSP_2899 EMBL ACT72688.1
OrganismEscherichia coli O157:H7 (strain TW14359 / EHEC) [Complete proteome] [HAMAP] EMBL ACT72688.1
Taxonomic identifier544404 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228 SAAS SAAS000417

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228 SAAS SAAS000417

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228 SAAS SAAS000417

Sequence similarities

Belongs to the Thz kinase family. HAMAP-Rule MF_00228

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site501Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00228
Binding site1251ATP By similarity HAMAP-Rule MF_00228
Binding site1711ATP By similarity HAMAP-Rule MF_00228
Binding site1981Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00228

Sequences

Sequence LengthMass (Da)Tools
C6V1K0 [UniParc].

Last modified September 22, 2009. Version 1.
Checksum: A28BF773FD2AD29F

FASTA26227,355
        10         20         30         40         50         60 
MQVDLLSSAQ SAHTLHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASQF 

        70         80         90        100        110        120 
AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP VAVGALDYRR HFCHELLSFK 

       130        140        150        160        170        180 
PAAIRGNASE IMALAGVANG GRGVDTTDAA VNAIPAAQTL ARETGAIVVV TGEVDYVTDG 

       190        200        210        220        230        240 
HRAVGIHGGD PLMTKVVGTG CALSAVVAAC CALPGDMLEN VASACHWMKQ AGERAVARSE 

       250        260 
GPGSFVPHFL DALWQLTQEV QA

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References

[1]"Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhance virulence."
Kulasekara B.R., Jacobs M., Zhou Y., Wu Z., Sims E., Saenphimmachak C., Rohmer L., Ritchie J.M., Radey M., McKevitt M., Freeman T.L., Hayden H., Haugen E., Gillett W., Fong C., Chang J., Beskhlebnaya V., Waldor M.K. expand/collapse author list , Samadpour M., Whittam T.S., Kaul R., Brittnacher M., Miller S.I.
Infect. Immun. 77:3713-3721(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TW14359 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001368 Genomic DNA. Translation: ACT72688.1.
RefSeqYP_003078764.1. NC_013008.1.

3D structure databases

ProteinModelPortalC6V1K0.
SMRC6V1K0. Positions 4-261.
ModBaseSearch...

Protein-protein interaction databases

STRING544404.ECSP_2899.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACT72688; ACT72688; ECSP_2899.
GeneID8217465.
KEGGetw:ECSP_2899.
PATRIC18389829. VBIEscCol9396_2946.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
ProtClustDBPRK09355.

Enzyme and pathway databases

UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC6V1K0_ECO5T
AccessionPrimary (citable) accession number: C6V1K0
Entry history
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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