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Protein

Secreted effector protein EspF(U)

Gene

espF(U)

Organism
Escherichia coli O157:H7 (strain TW14359 / EHEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processVirulence

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted effector protein EspF(U)
Alternative name(s):
EspF-like protein encoded on prophage U
Tir-cytoskeleton coupling protein TccP
Gene namesi
Name:espF(U)
Synonyms:tccP
Ordered Locus Names:ECSP_2584
OrganismiEscherichia coli O157:H7 (strain TW14359 / EHEC)
Taxonomic identifieri544404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Secreted By similarity
  • Host cytoplasm By similarity
  • Note: Secreted via the type III secretion system (TTSS). In host cells, localizes to the tip of the actin pedestal (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004139861 – 337Secreted effector protein EspF(U)Add BLAST337

Proteomic databases

PRIDEiC6UYI3.

Interactioni

Subunit structurei

Interacts with host BAIAP2 and host WASL/N-WASP. Can also interact with host proteins BAIAP2L1 and WAS/WASP (By similarity).By similarity

Structurei

3D structure databases

SMRiC6UYI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati96 – 1421Add BLAST47
Repeati143 – 1892Add BLAST47
Repeati190 – 2363Add BLAST47
Repeati237 – 2834Add BLAST47
Repeati284 – 3305Add BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 3305 X 48 AA approximate tandem repeatsAdd BLAST235

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi107 – 316Pro-richAdd BLAST210

Domaini

The N-terminal 21 amino acids are necessary and sufficient for translocation into the host cell. The C-terminal region, composed of several highly conserved proline-rich repeats, interacts with the SH3 domain of BAIAP2 and BAIAP2L1, and the GTPase binding domain (GBD) of WASL/N-WASP and WAS/WASP. The N-terminal translocation signal and two proline-rich repeats are sufficient for triggering actin polymerization, but each additional repeat gives higher activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the EspF(U)/TccP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000230854.
KOiK12789.
OMAiIQHLAEH.

Family and domain databases

InterProiView protein in InterPro
IPR006891. T3SS_EspF.
PfamiView protein in Pfam
PF04806. EspF. 6 hits.

Sequencei

Sequence statusi: Complete.

C6UYI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINNVSSLFP TVNRNITAVY KKSSFSVSPQ KITLNPVKIS SPFSPSSSSI
60 70 80 90 100
SATTLFRAPN AHSASFHRQS TAESSLHQQL PNVRQRLIQH LAEHGIKPAR
110 120 130 140 150
SMAEHIPPAP NWPAPPPPVQ NEQSRPLPDV AQRLVQHLAE HGIQPARNMA
160 170 180 190 200
EHIPPAPNWP APPLPVQNEQ SRPLPDVAQR LVQHLAEHGI QPARSMAEHI
210 220 230 240 250
PPAPNWPAPP PPVQNEQSRP LPDVAQRLMQ HLAEHGIQPA RNMAEHIPPA
260 270 280 290 300
PNWPAPTPPV QNEQSRPLPD VAQRLMQHLA EHGIQPARNM AEHIPPAPNW
310 320 330
PAPTPPVQNE QSRPLPDVAQ RLMQHLAEHG INTSKRS
Length:337
Mass (Da):37,192
Last modified:September 22, 2009 - v1
Checksum:i49A1BE2EA220E1C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001368 Genomic DNA. Translation: ACT72391.1.
RefSeqiWP_010917831.1. NC_013008.1.

Genome annotation databases

EnsemblBacteriaiACT72391; ACT72391; ECSP_2584.
KEGGietw:ECSP_2584.

Similar proteinsi

Entry informationi

Entry nameiESPFU_ECO5T
AccessioniPrimary (citable) accession number: C6UYI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: September 22, 2009
Last modified: June 7, 2017
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families