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Protein

Phosphoheptose isomerase

Gene

lpcA

Organism
Escherichia coli O157:H7 (strain TW14359 / EHEC)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+SAAS annotationNote: Binds 1 zinc ion per subunit.SAAS annotation
  • Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611ZincUniRule annotation
Metal bindingi65 – 651ZincUniRule annotation
Binding sitei65 – 651SubstrateUniRule annotation
Binding sitei124 – 1241SubstrateUniRule annotation
Metal bindingi172 – 1721ZincUniRule annotation
Binding sitei172 – 1721SubstrateUniRule annotation
Metal bindingi180 – 1801ZincUniRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. D-sedoheptulose 7-phosphate isomerase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-glycero-D-manno-heptose 7-phosphate biosynthetic process Source: UniProtKB-UniPathway
  2. lipopolysaccharide core region biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Biological processi

Carbohydrate metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciECOL544404:GKCX-254-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoheptose isomeraseUniRule annotationSAAS annotation (EC:5.3.1.28UniRule annotationSAAS annotation)
Alternative name(s):
Sedoheptulose 7-phosphate isomeraseUniRule annotation
Gene namesi
Name:lpcAImported
Synonyms:gmhAUniRule annotation
Ordered Locus Names:ECSP_0254Imported
OrganismiEscherichia coli O157:H7 (strain TW14359 / EHEC)Imported
Taxonomic identifieri544404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000002056: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Proteomic databases

PRIDEiC6UX01.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi544404.ECSP_0254.

Structurei

3D structure databases

ProteinModelPortaliC6UX01.
SMRiC6UX01. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 192156SISUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 543Substrate bindingUniRule annotation
Regioni93 – 942Substrate bindingUniRule annotation
Regioni119 – 1213Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the SIS family. GmhA subfamily.UniRule annotation
Contains 1 SIS domain.UniRule annotation
Contains SIS domain.SAAS annotation

Phylogenomic databases

eggNOGiCOG0279.
HOGENOMiHOG000237571.
KOiK03271.
OMAiGVKNDVL.
OrthoDBiEOG6384PC.

Family and domain databases

HAMAPiMF_00067. GmhA.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6UX01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYQDLIRNEL NEAAETLANF LKDDANIHAI QRAAVLLADS FKAGGKVLSC
60 70 80 90 100
GNGGSHCDAM HFAEELTGRY RENRPGYPAI AISDVSHISC VGNDFGFNDI
110 120 130 140 150
FSRYVEAVGR EGDVLLGIST SGNSANVIKA IAAAREKGMK VITLTGKDGG
160 170 180 190
KMAGTADIEI RVPHFGYADR IQEIHIKVIH ILIQLIEKEM VK
Length:192
Mass (Da):20,815
Last modified:September 22, 2009 - v1
Checksum:i7A2C05E1079108B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001368 Genomic DNA. Translation: ACT70142.1.
RefSeqiYP_003076218.1. NC_013008.1.

Genome annotation databases

EnsemblBacteriaiACT70142; ACT70142; ECSP_0254.
GeneIDi8214820.
KEGGietw:ECSP_0254.
PATRICi18384342. VBIEscCol9396_0253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001368 Genomic DNA. Translation: ACT70142.1.
RefSeqiYP_003076218.1. NC_013008.1.

3D structure databases

ProteinModelPortaliC6UX01.
SMRiC6UX01. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi544404.ECSP_0254.

Proteomic databases

PRIDEiC6UX01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACT70142; ACT70142; ECSP_0254.
GeneIDi8214820.
KEGGietw:ECSP_0254.
PATRICi18384342. VBIEscCol9396_0253.

Phylogenomic databases

eggNOGiCOG0279.
HOGENOMiHOG000237571.
KOiK03271.
OMAiGVKNDVL.
OrthoDBiEOG6384PC.

Enzyme and pathway databases

BioCyciECOL544404:GKCX-254-MONOMER.

Family and domain databases

HAMAPiMF_00067. GmhA.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhance virulence."
    Kulasekara B.R., Jacobs M., Zhou Y., Wu Z., Sims E., Saenphimmachak C., Rohmer L., Ritchie J.M., Radey M., McKevitt M., Freeman T.L., Hayden H., Haugen E., Gillett W., Fong C., Chang J., Beskhlebnaya V., Waldor M.K.
    , Samadpour M., Whittam T.S., Kaul R., Brittnacher M., Miller S.I.
    Infect. Immun. 77:3713-3721(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW14359 / EHECImported.

Entry informationi

Entry nameiC6UX01_ECO5T
AccessioniPrimary (citable) accession number: C6UX01
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.