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C6UQ52

- C6UQ52_ECO5T

UniProt

C6UQ52 - C6UQ52_ECO5T

Protein

Succinyl-diaminopimelate desuccinylase

Gene

dapE

Organism
Escherichia coli O157:H7 (strain TW14359 / EHEC)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.UniRule annotationSAAS annotation

    Catalytic activityi

    N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 Co2+ ion per subunit.UniRule annotationSAAS annotation
    Binds 1 Zn2+ ion per subunit.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Cobalt or zinc 1UniRule annotation
    Active sitei68 – 681UniRule annotation
    Metal bindingi99 – 991Cobalt or zinc 1UniRule annotation
    Metal bindingi99 – 991Cobalt or zinc 2UniRule annotation
    Active sitei133 – 1331Proton acceptorUniRule annotation
    Metal bindingi134 – 1341Cobalt or zinc 2UniRule annotation
    Metal bindingi162 – 1621Cobalt or zinc 1UniRule annotation
    Metal bindingi348 – 3481Cobalt or zinc 2UniRule annotation

    GO - Molecular functioni

    1. cobalt ion binding Source: UniProtKB-HAMAP
    2. metallopeptidase activity Source: InterPro
    3. succinyl-diaminopimelate desuccinylase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    CobaltUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciECOL544404:GKCX-3406-MONOMER.
    UniPathwayiUPA00034; UER00021.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinyl-diaminopimelate desuccinylaseUniRule annotation (EC:3.5.1.18UniRule annotation)
    Short name:
    SDAP desuccinylaseUniRule annotation
    Alternative name(s):
    N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolaseUniRule annotation
    Gene namesi
    Name:dapEUniRule annotationImported
    Ordered Locus Names:ECSP_3410Imported
    OrganismiEscherichia coli O157:H7 (strain TW14359 / EHEC)Imported
    Taxonomic identifieri544404 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000002056: Chromosome

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi544404.ECSP_3410.

    Structurei

    3D structure databases

    ProteinModelPortaliC6UQ52.
    SMRiC6UQ52. Positions 2-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family. DapE subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0624.
    HOGENOMiHOG000243770.
    KOiK01439.
    OMAiHAGRLAF.
    OrthoDBiEOG60651W.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    HAMAPiMF_01690. DapE.
    InterProiIPR001261. ArgE/DapE_CS.
    IPR005941. DapE_proteobac.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01246. dapE_proteo. 1 hit.
    PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6UQ52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCPVIELTQ QLIRRPSLSP DDAGCQALLI ERLQAIGFTV ERMDFADTQN    50
    FWAWRGQGET LAFAGHTDVV PPGDADRWIN PPFEPTIRDG MLFGRGAADM 100
    KGSLAAMVVA AERFVAQHPN HTGRLAFLIT SDEEASAHNG TVKAVEALMA 150
    RNERLDYCLV GEPSSIEVVG DVVKNGRRGS LTCNLTIHGV QGHVAYPHLA 200
    DNPVHRAAPF LNELVAIEWD QGNEFFPATS MQIANIQAGT GSNNVIPGEL 250
    FVQFNFRFST ELTDEMIKAQ VLALLEKHQL RYTVDWWLSG QPFLTARGKL 300
    VDAVVNAVEH YNEIKPQLLT TGGTSDGRFI ARMGAQVVEL GPVNATIHKI 350
    NECVNAADLQ LLARMYQRIM EQLVA 375
    Length:375
    Mass (Da):41,241
    Last modified:September 22, 2009 - v1
    Checksum:iB249E6B09D84F2CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001368 Genomic DNA. Translation: ACT73177.1.
    RefSeqiYP_003079253.1. NC_013008.1.

    Genome annotation databases

    EnsemblBacteriaiACT73177; ACT73177; ECSP_3410.
    GeneIDi8217976.
    KEGGietw:ECSP_3410.
    PATRICi18390894. VBIEscCol9396_3464.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001368 Genomic DNA. Translation: ACT73177.1 .
    RefSeqi YP_003079253.1. NC_013008.1.

    3D structure databases

    ProteinModelPortali C6UQ52.
    SMRi C6UQ52. Positions 2-374.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 544404.ECSP_3410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACT73177 ; ACT73177 ; ECSP_3410 .
    GeneIDi 8217976.
    KEGGi etw:ECSP_3410.
    PATRICi 18390894. VBIEscCol9396_3464.

    Phylogenomic databases

    eggNOGi COG0624.
    HOGENOMi HOG000243770.
    KOi K01439.
    OMAi HAGRLAF.
    OrthoDBi EOG60651W.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00021 .
    BioCyci ECOL544404:GKCX-3406-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    HAMAPi MF_01690. DapE.
    InterProi IPR001261. ArgE/DapE_CS.
    IPR005941. DapE_proteobac.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01246. dapE_proteo. 1 hit.
    PROSITEi PS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhance virulence."
      Kulasekara B.R., Jacobs M., Zhou Y., Wu Z., Sims E., Saenphimmachak C., Rohmer L., Ritchie J.M., Radey M., McKevitt M., Freeman T.L., Hayden H., Haugen E., Gillett W., Fong C., Chang J., Beskhlebnaya V., Waldor M.K.
      , Samadpour M., Whittam T.S., Kaul R., Brittnacher M., Miller S.I.
      Infect. Immun. 77:3713-3721(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TW14359 / EHECImported.

    Entry informationi

    Entry nameiC6UQ52_ECO5T
    AccessioniPrimary (citable) accession number: C6UQ52
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3