C6UM53 (C6UM53_ECOBR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase HAMAP-Rule MF_00033
EC=2.4.1.227 HAMAP-Rule MF_00033

Alternative name(s):
Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase HAMAP-Rule MF_00033

Gene names

Name:	murG HAMAP-Rule MF_00033
Ordered Locus Names:	ECB_00091

Organism

Escherichia coli (strain B / REL606) [Complete proteome] [HAMAP] EMBL ACT37784.1

Taxonomic identifier

413997 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli B

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) By similarity. HAMAP-Rule MF_00033 SAAS SAAS006009
Catalytic activity	UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP-Rule MF_00033 SAAS SAAS006009
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00033 SAAS SAAS006009
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00033.
Sequence similarities	Belongs to the glycosyltransferase 28 family. MurG subfamily. HAMAP-Rule MF_00033

Ontologies

Keywords
Biological process	Cell cycle Cell division HAMAP-Rule MF_00033 SAAS SAAS006009 Cell shape Cell wall biogenesis/degradation HAMAP-Rule MF_00033 SAAS SAAS006009 Peptidoglycan synthesis HAMAP-Rule MF_00033 SAAS SAAS006009
Cellular component	Cell inner membrane HAMAP-Rule MF_00033 Cell membrane Membrane
Molecular function	Glycosyltransferase HAMAP-Rule MF_00033 SAAS SAAS006009 EMBL ACT37784.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C6UM53 [UniParc]. Last modified September 22, 2009. Version 1. Checksum: DE347361A7B9293A	FASTA	355	37,815
	10 20 30 40 50 60 MSGQGKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEADLV PKHGIEIDFI 70 80 90 100 110 120 RISGLRGKGI KALIAAPLRI FNAWRQARAI MKAYKPDVVL GMGGYVSGPG GLAAWSLGIP 130 140 150 160 170 180 VVLHEQNGIA GLTNKWLAKI ATKVMQAFPG AFPNAEVVGN PVRTDVLALP LPQQRLAGRE 190 200 210 220 230 240 GPVRVLVVGG SQGARILNQT MPQVAAKLGD SVTIWHQSGK GSQQSVEQAY AEAGQPQHKV 250 260 270 280 290 300 TEFIDDMAAA YAWADVVVCR SGALTVSEIA AAGLPALFVP FQHKDRQQYW NALPLEKAGA 310 320 330 340 350 AKIIEQPQLS VDAVANTLAG WSRETLLTMA ERARAASIPD ATERVANEVS RVARA

References

[1]

"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)." Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F. J. Mol. Biol. 394:644-652(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / REL606.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000819 Genomic DNA. Translation: ACT37784.1.
RefSeq	YP_003043320.1. NC_012967.1.
3D structure databases
ProteinModelPortal	C6UM53.
SMR	C6UM53. Positions 6-355.
ModBase	Search...
Protein-protein interaction databases
STRING	413997.ECB_00091.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACT37784; ACT37784; ECB_00091.
GeneID	8175079.
KEGG	ebr:ECB_00091.
PATRIC	18232072. VBIEscCol118196_0097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0707.
HOGENOM	HOG000218321.
KO	K02563.
OMA	DDHQTKN.
ProtClustDB	PRK00726.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
HAMAP	MF_00033. MurG.
InterPro	IPR006009. GlcNAc_MurG. IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. [Graphical view]
PANTHER	PTHR21015:SF22. PTHR21015:SF22. 1 hit.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. [Graphical view]
TIGRFAMs	TIGR01133. murG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C6UM53_ECOBR

Accession

Primary (citable) accession number: C6UM53

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)