C6UD50 (C6UD50_ECOBR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase HAMAP MF_00126
EC=6.1.1.18 HAMAP MF_00126

Alternative name(s):
Glutaminyl-tRNA synthetase HAMAP MF_00126

Gene names

Name:	glnS HAMAP MF_00126
Ordered Locus Names:	ECB_00637

Organism

Escherichia coli (strain B / REL606) [Complete proteome] [HAMAP] EMBL ACT38320.1

Taxonomic identifier

413997 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00126

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00126
Cellular component	Cytoplasm HAMAP MF_00126
Ligand	ATP-binding HAMAP MF_00126 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00126 EMBL ACT38320.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	34 – 44	11	"HIGH" region By similarity HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region By similarity HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity HAMAP MF_00126

Sequences

	Sequence		Length	Mass (Da)
	C6UD50 [UniParc]. Last modified September 22, 2009. Version 1. Checksum: E720164EF990F335	FASTA	554	63,478
	10 20 30 40 50 60 MSEAEARPTN FIRQIIDEDL ASGKHTTVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYKG 70 80 90 100 110 120 QCNLRFDDTN PVKEDIEYVE SIKNDVEWLG FHWSGNVRYS SDYFDQLHAY AIELINKGLA 130 140 150 160 170 180 YVDELTPEQI REYRGTLTQP GKNSPYRDRS VEENLALFEK MRAGGFEEGK ACLRAKIDMA 190 200 210 220 230 240 SPFIVMRDPV LYRIKFAEHH QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNITI PVHPRQYEFS RLNLEYTVMS KRKLNLLVTD KHVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYTAASIREF CKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV KLVIENYQGE 370 380 390 400 410 420 GEMVTMPNHP NKPEMGSRQV PFSGEIWIDR ADFREEANKQ YKRLVLGKEV RLRNAYVIKA 430 440 450 460 470 480 ERVEKDAEGN ITTIFCTYDA DTLSKDPADG RKVKGVIHWV SAAHALPVEI RLYDRLFSVP 490 500 510 520 530 540 NPGAADDFLS VINPESLVIK QGFAEPSLKD AVAGKAFQFE REGYFCLDSR HSTAEKPVFN 550 RTVGLRDTWA KVGE

References

[1]

"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)." Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F. J. Mol. Biol. 394:644-652(2009) [PubMed: 19786035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000819 Genomic DNA. Translation: ACT38320.1.
RefSeq	YP_003043856.1. NC_012967.1.
3D structure databases
ProteinModelPortal	C6UD50.
SMR	C6UD50. Positions 9-548.
ModBase	Search...
Protein-protein interaction databases
STRING	C6UD50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000164072; EBESCP00000150928; EBESCG00000159935.
GeneID	8175502.
GenomeReviews	Gene locus ECB_00637 in contig CP000819_GR.
KEGG	ebr:ECB_00637.
PATRIC	18233266. VBIEscCol118196_0677.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009048.
ProtClustDB	PRK05347.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6UD50_ECOBR

Accession

Primary (citable) accession number: C6UD50

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)