C6UBQ2 (C6UBQ2_ECOBR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit HAMAP-Rule MF_00444 RuleBase RU003567
EC=3.4.21.92 HAMAP-Rule MF_00444 RuleBase RU000549

Alternative name(s):
Endopeptidase Clp HAMAP-Rule MF_00444

Gene names

Name:	clpP HAMAP-Rule MF_00444
Ordered Locus Names:	ECB_00388

Organism

Escherichia coli (strain B / REL606) [Complete proteome] [HAMAP] EMBL ACT38078.1

Taxonomic identifier

413997 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli B

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP-Rule MF_00444 RuleBase RU000550
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP-Rule MF_00444 RuleBase RU000549
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. HAMAP-Rule MF_00444 RuleBase RU003567

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_00444
Ligand	ATP-binding HAMAP-Rule MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP-Rule MF_00444 RuleBase RU000549
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	111	1	By similarity HAMAP-Rule MF_00444
	Active site	136	1	By similarity HAMAP-Rule MF_00444

Sequences

	Sequence		Length	Mass (Da)
	C6UBQ2 [UniParc]. Last modified September 22, 2009. Version 1. Checksum: A7843D036C8CB3C2	FASTA	207	23,187
	10 20 30 40 50 60 MSYSGERDNF APHMALVPMV IEQTSRGERS FDIYSRLLKE RVIFLTGQVE DHMANLIVAQ 70 80 90 100 110 120 MLFLEAENPE KDIYLYINSP GGVITAGMSI YDTMQFIKPD VSTICMGQAA SMGAFLLTAG 130 140 150 160 170 180 AKGKRFCLPN SRVMIHQPLG GYQGQATDIE IHAREILKVK GRMNELMALH TGQSLEQIER 190 200 DTERDRFLSA PEAVEYGLVD SILTHRN

References

[1]

"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)." Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F. J. Mol. Biol. 394:644-652(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / REL606.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000819 Genomic DNA. Translation: ACT38078.1.
RefSeq	YP_003043614.1. NC_012967.1.
3D structure databases
ProteinModelPortal	C6UBQ2.
SMR	C6UBQ2. Positions 16-207.
ModBase	Search...
Protein-protein interaction databases
STRING	413997.ECB_00388.
Proteomic databases
PRIDE	C6UBQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACT38078; ACT38078; ECB_00388.
GeneID	8175309.
KEGG	ebr:ECB_00388.
PATRIC	18232732. VBIEscCol118196_0418.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HOG000285833.
KO	K01358.
OMA	LVHPPQA.
ProtClustDB	PRK00277.
Family and domain databases
HAMAP	MF_00444. ClpP.
InterPro	IPR001907. ClpP. IPR023562. ClpP/TepA. IPR018215. ClpP_AS. [Graphical view]
PANTHER	PTHR10381. PTHR10381. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. clpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6UBQ2_ECOBR

Accession

Primary (citable) accession number: C6UBQ2

Entry history

Integrated into UniProtKB/TrEMBL:	September 22, 2009
Last sequence update:	September 22, 2009
Last modified:	May 29, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)