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C6KSX0

- PF12_PLAF7

UniProt

C6KSX0 - PF12_PLAF7

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Protein

Merozoite surface protein P12

Gene

PF12

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. entry into host cell Source: GeneDB_Pfalciparum
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Merozoite surface protein P12
Cleaved into the following chain:
Gene namesi
Name:PF12
ORF Names:PFF0615c
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)
ProteomesiUP000001450: Chromosome 6

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0612700.

Subcellular locationi

Chain Merozoite surface protein P12 : Cell surface. Cell membrane Curated; Lipid-anchorGPI-anchor Curated
Note: Present on the surface of merozoite.1 Publication
Chain Merozoite surface protein P12, processed form : Cell surface. Cell membrane
Note: Shed from the merozoite surface.1 Publication

GO - Cellular componenti

  1. anchored component of plasma membrane Source: GeneDB_Pfalciparum
  2. apical part of cell Source: GeneDB_Pfalciparum
  3. cell surface Source: GeneDB_Pfalciparum
  4. symbiont-containing vacuolar space Source: GeneDB_Pfalciparum
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Parasites grow at normal rates in vitro.1 Publication

Keywords - Diseasei

Malaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 322297Merozoite surface protein P12PRO_0000423557Add
BLAST
Chaini26 – ?Merozoite surface protein P12, processed formPRO_0000423558
Propeptidei323 – 34725Removed in mature formSequence AnalysisPRO_0000423559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi31 ↔ 531 Publication
Disulfide bondi67 ↔ 1381 Publication
Disulfide bondi81 ↔ 1361 Publication
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi179 ↔ 2111 Publication
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi225 ↔ 2861 Publication
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi236 ↔ 2841 Publication
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
Lipidationi322 – 3221GPI-anchor amidated asparagineSequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Processed into a soluble form.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Developmental stagei

Specifically present in asexual blood stage parasites. First detected in the trophozoite stage, 30-40 hours post-invasion (HPI) and the proteins reaches peak expression in schizont stage, 40-48 HPI.2 Publications

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with PF41. May form an antiparallel heterodimer with PF41.

Protein-protein interaction databases

STRINGi5833.PFF0615c-1.

Structurei

Secondary structure

1
347
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 323Combined sources
Turni33 – 364Combined sources
Beta strandi50 – 556Combined sources
Beta strandi62 – 665Combined sources
Beta strandi70 – 723Combined sources
Beta strandi75 – 784Combined sources
Beta strandi119 – 1235Combined sources
Beta strandi134 – 1407Combined sources
Beta strandi161 – 1677Combined sources
Beta strandi176 – 1805Combined sources
Beta strandi187 – 1926Combined sources
Helixi195 – 1984Combined sources
Beta strandi209 – 2157Combined sources
Beta strandi220 – 2245Combined sources
Beta strandi230 – 2334Combined sources
Beta strandi239 – 2457Combined sources
Beta strandi247 – 2493Combined sources
Beta strandi257 – 2626Combined sources
Turni263 – 2664Combined sources
Beta strandi267 – 2715Combined sources
Beta strandi274 – 2763Combined sources
Beta strandi280 – 2878Combined sources
Beta strandi294 – 3007Combined sources
Beta strandi302 – 3043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YMOX-ray1.90A28-304[»]
ProteinModelPortaliC6KSX0.
SMRiC6KSX0. Positions 29-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 1721466-Cys 1Add
BLAST
Domaini175 – 3051316-Cys 2Add
BLAST

Sequence similaritiesi

Contains 2 6-Cys domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000281492.
InParanoidiC6KSX0.
OMAiESHYSAN.
PhylomeDBiC6KSX0.

Family and domain databases

InterProiIPR010884. Sexual_stage_antigen_S48/45.
[Graphical view]
PfamiPF07422. s48_45. 2 hits.
[Graphical view]
SMARTiSM00970. s48_45. 2 hits.
[Graphical view]
PROSITEiPS51701. 6_CYS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C6KSX0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKLSKKYCL GISFVLYILL SVCEGHKNLT CDFNDVYKLE FHPNQQTSVT
60 70 80 90 100
KLCNLTPNVL EKVTIKCGSD KLNYNLYPPT CFEEVYASRN MMHLKKIKEF
110 120 130 140 150
VIGSSMFMRR SLTPNKINEV SFRIPPNMMP EKPIYCFCEN KKTITINGSN
160 170 180 190 200
GNPSSKKDII NRGIVEIIIP SLNEKVKGCD FTTSESTIFS KGYSINEISN
210 220 230 240 250
KSSNNQQDIV CTVKAHANDL IGFKCPSNYS VEPHDCFVSA FNLSGKNENL
260 270 280 290 300
ENKLKLTNII MDHYNNTFYS RLPSLISDNW KFFCVCSKDN EKKLVFTVEA
310 320 330 340
SISSSNTKLA SRDNTYQDYI SNSSFLTLSS YCAFITFIIT SFLSFIL
Length:347
Mass (Da):39,434
Last modified:September 1, 2009 - v1
Checksum:i0950614A8565DEA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844505 Genomic DNA. Translation: CAG25366.1.
RefSeqiXP_966114.1. XM_961021.1.

Genome annotation databases

EnsemblProtistsiPFF0615c:mRNA; PFF0615c:pep; PFF0615c.
GeneIDi3885775.
KEGGipfa:PFF0615c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844505 Genomic DNA. Translation: CAG25366.1 .
RefSeqi XP_966114.1. XM_961021.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YMO X-ray 1.90 A 28-304 [» ]
ProteinModelPortali C6KSX0.
SMRi C6KSX0. Positions 29-305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5833.PFF0615c-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi PFF0615c:mRNA ; PFF0615c:pep ; PFF0615c .
GeneIDi 3885775.
KEGGi pfa:PFF0615c.

Organism-specific databases

EuPathDBi PlasmoDB:PF3D7_0612700.

Phylogenomic databases

HOGENOMi HOG000281492.
InParanoidi C6KSX0.
OMAi ESHYSAN.
PhylomeDBi C6KSX0.

Family and domain databases

InterProi IPR010884. Sexual_stage_antigen_S48/45.
[Graphical view ]
Pfami PF07422. s48_45. 2 hits.
[Graphical view ]
SMARTi SM00970. s48_45. 2 hits.
[Graphical view ]
PROSITEi PS51701. 6_CYS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  2. "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
    Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z.
    , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
    Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  3. "Predicted disulfide-bonded structures for three uniquely related proteins of Plasmodium falciparum, Pfs230, Pfs48/45 and Pf12."
    Carter R., Coulson A., Bhatti S., Taylor B.J., Elliott J.F.
    Mol. Biochem. Parasitol. 71:203-210(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum."
    Sanders P.R., Gilson P.R., Cantin G.T., Greenbaum D.C., Nebl T., Carucci D.J., McConville M.J., Schofield L., Hodder A.N., Yates J.R. III, Crabb B.S.
    J. Biol. Chem. 280:40169-40176(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  5. "Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum."
    Gilson P.R., Nebl T., Vukcevic D., Moritz R.L., Sargeant T., Speed T.P., Schofield L., Crabb B.S.
    Mol. Cell. Proteomics 5:1286-1299(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.
  6. Cited for: SUBCELLULAR LOCATION, CLEAVAGE, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, INTERACTION WITH PF41.
  7. "Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12) reveals a unique interdomain organization and the potential for an antiparallel arrangement with Pf41."
    Tonkin M.L., Arredondo S.A., Loveless B.C., Serpa J.J., Makepeace K.A., Sundar N., Petrotchenko E.V., Miller L.H., Grigg M.E., Boulanger M.J.
    J. Biol. Chem. 288:12805-12817(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 28-304, DISULFIDE BOND, SUBCELLULAR LOCATION, INTERACTION WITH PF41.

Entry informationi

Entry nameiPF12_PLAF7
AccessioniPrimary (citable) accession number: C6KSX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Does not have erythrocyte-receptor-binding ability.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3