Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

C6KSX0 (PF12_PLAF7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Merozoite surface protein P12

Cleaved into the following chain:

  1. Merozoite surface protein P12, processed form
Gene names
Name:PF12
ORF Names:PFF0615c
OrganismPlasmodium falciparum (isolate 3D7) [Reference proteome]
Taxonomic identifier36329 [NCBI]
Taxonomic lineageEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Heterodimer; heterodimerizes with PF41. May form an antiparallel heterodimer with PF41.

Subcellular location

Merozoite surface protein P12: Cell surface. Cell membrane; Lipid-anchorGPI-anchor Probable. Note: Present on the surface of merozoite (Ref.6). Ref.4 Ref.5 Ref.6 Ref.7

Merozoite surface protein P12, processed form: Cell surface. Cell membrane. Note: Shed from the merozoite surface (Ref.6). Ref.4 Ref.6 Ref.7

Developmental stage

Specifically present in asexual blood stage parasites. First detected in the trophozoite stage, 30-40 hours post-invasion (HPI) and the proteins reaches peak expression in schizont stage, 40-48 HPI. Ref.4 Ref.6

Post-translational modification

Processed into a soluble form (Ref.6).

Disruption phenotype

No visible phenotype. Parasites grow at normal rates in vitro. Ref.6

Miscellaneous

Does not have erythrocyte-receptor-binding ability (Ref.6).

Sequence similarities

Contains 2 6-Cys domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 322297Merozoite surface protein P12
PRO_0000423557
Chain26 – ?Merozoite surface protein P12, processed formPRO_0000423558
Propeptide323 – 34725Removed in mature form Potential
PRO_0000423559

Regions

Domain27 – 1721466-Cys 1
Domain175 – 3051316-Cys 2

Amino acid modifications

Lipidation3221GPI-anchor amidated asparagine Potential
Glycosylation281N-linked (GlcNAc...) Potential
Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2281N-linked (GlcNAc...) Potential
Glycosylation2421N-linked (GlcNAc...) Potential
Glycosylation2651N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 53 Ref.7
Disulfide bond67 ↔ 138 Ref.7
Disulfide bond81 ↔ 136 Ref.7
Disulfide bond179 ↔ 211 Ref.7
Disulfide bond225 ↔ 286 Ref.7
Disulfide bond236 ↔ 284 Ref.7

Secondary structure

.............................................. 347
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
C6KSX0 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: 0950614A8565DEA5

FASTA34739,434
        10         20         30         40         50         60 
MIKLSKKYCL GISFVLYILL SVCEGHKNLT CDFNDVYKLE FHPNQQTSVT KLCNLTPNVL 

        70         80         90        100        110        120 
EKVTIKCGSD KLNYNLYPPT CFEEVYASRN MMHLKKIKEF VIGSSMFMRR SLTPNKINEV 

       130        140        150        160        170        180 
SFRIPPNMMP EKPIYCFCEN KKTITINGSN GNPSSKKDII NRGIVEIIIP SLNEKVKGCD 

       190        200        210        220        230        240 
FTTSESTIFS KGYSINEISN KSSNNQQDIV CTVKAHANDL IGFKCPSNYS VEPHDCFVSA 

       250        260        270        280        290        300 
FNLSGKNENL ENKLKLTNII MDHYNNTFYS RLPSLISDNW KFFCVCSKDN EKKLVFTVEA 

       310        320        330        340 
SISSSNTKLA SRDNTYQDYI SNSSFLTLSS YCAFITFIIT SFLSFIL 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the human malaria parasite Plasmodium falciparum."
Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W., Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D., Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S. expand/collapse author list , Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M., Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A., Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I., Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J., Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.
Nature 419:498-511(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate 3D7.
[2]"Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z. expand/collapse author list , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate 3D7.
[3]"Predicted disulfide-bonded structures for three uniquely related proteins of Plasmodium falciparum, Pfs230, Pfs48/45 and Pf12."
Carter R., Coulson A., Bhatti S., Taylor B.J., Elliott J.F.
Mol. Biochem. Parasitol. 71:203-210(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[4]"Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum."
Sanders P.R., Gilson P.R., Cantin G.T., Greenbaum D.C., Nebl T., Carucci D.J., McConville M.J., Schofield L., Hodder A.N., Yates J.R. III, Crabb B.S.
J. Biol. Chem. 280:40169-40176(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[5]"Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum."
Gilson P.R., Nebl T., Vukcevic D., Moritz R.L., Sargeant T., Speed T.P., Schofield L., Crabb B.S.
Mol. Cell. Proteomics 5:1286-1299(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR.
[6]"Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41."
Taechalertpaisarn T., Crosnier C., Bartholdson S.J., Hodder A.N., Thompson J., Bustamante L.Y., Wilson D.W., Sanders P.R., Wright G.J., Rayner J.C., Cowman A.F., Gilson P.R., Crabb B.S.
PLoS ONE 7:E41937-E41937(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, CLEAVAGE, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, INTERACTION WITH PF41.
[7]"Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12) reveals a unique interdomain organization and the potential for an antiparallel arrangement with Pf41."
Tonkin M.L., Arredondo S.A., Loveless B.C., Serpa J.J., Makepeace K.A., Sundar N., Petrotchenko E.V., Miller L.H., Grigg M.E., Boulanger M.J.
J. Biol. Chem. 288:12805-12817(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 28-304, DISULFIDE BOND, SUBCELLULAR LOCATION, INTERACTION WITH PF41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL844505 Genomic DNA. Translation: CAG25366.1.
RefSeqXP_966114.1. XM_961021.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YMOX-ray1.90A28-304[»]
ProteinModelPortalC6KSX0.
SMRC6KSX0. Positions 29-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5833.PFF0615c-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsPFF0615c:mRNA; PFF0615c:pep; PFF0615c.
GeneID3885775.
KEGGpfa:PFF0615c.

Organism-specific databases

EuPathDBPlasmoDB:PF3D7_0612700.

Phylogenomic databases

HOGENOMHOG000281492.
PhylomeDBC6KSX0.
ProtClustDBPTZ00442.

Family and domain databases

InterProIPR010884. Sexual_stage_antigen_S48/45.
[Graphical view]
PfamPF07422. s48_45. 2 hits.
[Graphical view]
SMARTSM00970. s48_45. 2 hits.
[Graphical view]
PROSITEPS51701. 6_CYS. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePF12_PLAF7
AccessionPrimary (citable) accession number: C6KSX0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: September 1, 2009
Last modified: April 16, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references