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C6KSX0

- PF12_PLAF7

UniProt

C6KSX0 - PF12_PLAF7

Protein

Merozoite surface protein P12

Gene

PF12

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    GO - Biological processi

    1. entry into host cell Source: GeneDB_Pfalciparum

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Merozoite surface protein P12
    Cleaved into the following chain:
    Gene namesi
    Name:PF12
    ORF Names:PFF0615c
    OrganismiPlasmodium falciparum (isolate 3D7)
    Taxonomic identifieri36329 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)
    ProteomesiUP000001450: Chromosome 6

    Organism-specific databases

    EuPathDBiPlasmoDB:PF3D7_0612700.

    Subcellular locationi

    Chain Merozoite surface protein P12 : Cell surface. Cell membrane Curated; Lipid-anchorGPI-anchor Curated
    Note: Present on the surface of merozoite.1 Publication
    Chain Merozoite surface protein P12, processed form : Cell surface. Cell membrane
    Note: Shed from the merozoite surface.1 Publication

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: GeneDB_Pfalciparum
    2. apical part of cell Source: GeneDB_Pfalciparum
    3. cell surface Source: GeneDB_Pfalciparum
    4. symbiont-containing vacuolar space Source: GeneDB_Pfalciparum

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Parasites grow at normal rates in vitro.1 Publication

    Keywords - Diseasei

    Malaria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 322297Merozoite surface protein P12PRO_0000423557Add
    BLAST
    Chaini26 – ?Merozoite surface protein P12, processed formPRO_0000423558
    Propeptidei323 – 34725Removed in mature formSequence AnalysisPRO_0000423559Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi31 ↔ 531 Publication
    Disulfide bondi67 ↔ 1381 Publication
    Disulfide bondi81 ↔ 1361 Publication
    Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi179 ↔ 2111 Publication
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi225 ↔ 2861 Publication
    Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi236 ↔ 2841 Publication
    Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
    Lipidationi322 – 3221GPI-anchor amidated asparagineSequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Processed into a soluble form.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Developmental stagei

    Specifically present in asexual blood stage parasites. First detected in the trophozoite stage, 30-40 hours post-invasion (HPI) and the proteins reaches peak expression in schizont stage, 40-48 HPI.2 Publications

    Interactioni

    Subunit structurei

    Heterodimer; heterodimerizes with PF41. May form an antiparallel heterodimer with PF41.

    Protein-protein interaction databases

    STRINGi5833.PFF0615c-1.

    Structurei

    Secondary structure

    1
    347
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 323
    Turni33 – 364
    Beta strandi50 – 556
    Beta strandi62 – 665
    Beta strandi70 – 723
    Beta strandi75 – 784
    Beta strandi119 – 1235
    Beta strandi134 – 1407
    Beta strandi161 – 1677
    Beta strandi176 – 1805
    Beta strandi187 – 1926
    Helixi195 – 1984
    Beta strandi209 – 2157
    Beta strandi220 – 2245
    Beta strandi230 – 2334
    Beta strandi239 – 2457
    Beta strandi247 – 2493
    Beta strandi257 – 2626
    Turni263 – 2664
    Beta strandi267 – 2715
    Beta strandi274 – 2763
    Beta strandi280 – 2878
    Beta strandi294 – 3007
    Beta strandi302 – 3043

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YMOX-ray1.90A28-304[»]
    ProteinModelPortaliC6KSX0.
    SMRiC6KSX0. Positions 29-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 1721466-Cys 1Add
    BLAST
    Domaini175 – 3051316-Cys 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 6-Cys domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOGENOMiHOG000281492.
    OMAiESHYSAN.
    PhylomeDBiC6KSX0.

    Family and domain databases

    InterProiIPR010884. Sexual_stage_antigen_S48/45.
    [Graphical view]
    PfamiPF07422. s48_45. 2 hits.
    [Graphical view]
    SMARTiSM00970. s48_45. 2 hits.
    [Graphical view]
    PROSITEiPS51701. 6_CYS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C6KSX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKLSKKYCL GISFVLYILL SVCEGHKNLT CDFNDVYKLE FHPNQQTSVT    50
    KLCNLTPNVL EKVTIKCGSD KLNYNLYPPT CFEEVYASRN MMHLKKIKEF 100
    VIGSSMFMRR SLTPNKINEV SFRIPPNMMP EKPIYCFCEN KKTITINGSN 150
    GNPSSKKDII NRGIVEIIIP SLNEKVKGCD FTTSESTIFS KGYSINEISN 200
    KSSNNQQDIV CTVKAHANDL IGFKCPSNYS VEPHDCFVSA FNLSGKNENL 250
    ENKLKLTNII MDHYNNTFYS RLPSLISDNW KFFCVCSKDN EKKLVFTVEA 300
    SISSSNTKLA SRDNTYQDYI SNSSFLTLSS YCAFITFIIT SFLSFIL 347
    Length:347
    Mass (Da):39,434
    Last modified:September 1, 2009 - v1
    Checksum:i0950614A8565DEA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL844505 Genomic DNA. Translation: CAG25366.1.
    RefSeqiXP_966114.1. XM_961021.1.

    Genome annotation databases

    EnsemblProtistsiPFF0615c:mRNA; PFF0615c:pep; PFF0615c.
    GeneIDi3885775.
    KEGGipfa:PFF0615c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL844505 Genomic DNA. Translation: CAG25366.1 .
    RefSeqi XP_966114.1. XM_961021.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YMO X-ray 1.90 A 28-304 [» ]
    ProteinModelPortali C6KSX0.
    SMRi C6KSX0. Positions 29-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5833.PFF0615c-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi PFF0615c:mRNA ; PFF0615c:pep ; PFF0615c .
    GeneIDi 3885775.
    KEGGi pfa:PFF0615c.

    Organism-specific databases

    EuPathDBi PlasmoDB:PF3D7_0612700.

    Phylogenomic databases

    HOGENOMi HOG000281492.
    OMAi ESHYSAN.
    PhylomeDBi C6KSX0.

    Family and domain databases

    InterProi IPR010884. Sexual_stage_antigen_S48/45.
    [Graphical view ]
    Pfami PF07422. s48_45. 2 hits.
    [Graphical view ]
    SMARTi SM00970. s48_45. 2 hits.
    [Graphical view ]
    PROSITEi PS51701. 6_CYS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate 3D7.
    2. "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
      Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z.
      , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
      Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate 3D7.
    3. "Predicted disulfide-bonded structures for three uniquely related proteins of Plasmodium falciparum, Pfs230, Pfs48/45 and Pf12."
      Carter R., Coulson A., Bhatti S., Taylor B.J., Elliott J.F.
      Mol. Biochem. Parasitol. 71:203-210(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum."
      Sanders P.R., Gilson P.R., Cantin G.T., Greenbaum D.C., Nebl T., Carucci D.J., McConville M.J., Schofield L., Hodder A.N., Yates J.R. III, Crabb B.S.
      J. Biol. Chem. 280:40169-40176(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    5. "Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum."
      Gilson P.R., Nebl T., Vukcevic D., Moritz R.L., Sargeant T., Speed T.P., Schofield L., Crabb B.S.
      Mol. Cell. Proteomics 5:1286-1299(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR.
    6. Cited for: SUBCELLULAR LOCATION, CLEAVAGE, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, INTERACTION WITH PF41.
    7. "Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12) reveals a unique interdomain organization and the potential for an antiparallel arrangement with Pf41."
      Tonkin M.L., Arredondo S.A., Loveless B.C., Serpa J.J., Makepeace K.A., Sundar N., Petrotchenko E.V., Miller L.H., Grigg M.E., Boulanger M.J.
      J. Biol. Chem. 288:12805-12817(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 28-304, DISULFIDE BOND, SUBCELLULAR LOCATION, INTERACTION WITH PF41.

    Entry informationi

    Entry nameiPF12_PLAF7
    AccessioniPrimary (citable) accession number: C6KSX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 18, 2013
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Does not have erythrocyte-receptor-binding ability.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3