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C6KSX0

- PF12_PLAF7

UniProt

C6KSX0 - PF12_PLAF7

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Protein
Merozoite surface protein P12
Gene
PF12, PFF0615c
Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Merozoite surface protein P12
Cleaved into the following chain:
Gene namesi
Name:PF12
ORF Names:PFF0615c
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)
ProteomesiUP000001450: Chromosome 6

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0612700.

Subcellular locationi

Chain Merozoite surface protein P12 : Cell surface. Cell membrane; Lipid-anchorGPI-anchor Inferred
Note: Present on the surface of merozoite (1 Publication).4 Publications
Chain Merozoite surface protein P12, processed form : Cell surface. Cell membrane
Note: Shed from the merozoite surface (1 Publication).3 Publications

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell surface Source: UniProtKB-SubCell
  3. membrane Source: GeneDB_Pfalciparum
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Parasites grow at normal rates in vitro.1 Publication

Keywords - Diseasei

Malaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 322297Merozoite surface protein P12
PRO_0000423557Add
BLAST
Chaini26 – ?Merozoite surface protein P12, processed formPRO_0000423558
Propeptidei323 – 34725Removed in mature form Reviewed prediction
PRO_0000423559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi31 ↔ 531 Publication
Disulfide bondi67 ↔ 1381 Publication
Disulfide bondi81 ↔ 1361 Publication
Glycosylationi147 – 1471N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi179 ↔ 2111 Publication
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi225 ↔ 2861 Publication
Glycosylationi228 – 2281N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi236 ↔ 2841 Publication
Glycosylationi242 – 2421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi265 – 2651N-linked (GlcNAc...) Reviewed prediction
Lipidationi322 – 3221GPI-anchor amidated asparagine Reviewed prediction
Glycosylationi322 – 3221N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Processed into a soluble form (1 Publication).

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Developmental stagei

Specifically present in asexual blood stage parasites. First detected in the trophozoite stage, 30-40 hours post-invasion (HPI) and the proteins reaches peak expression in schizont stage, 40-48 HPI.2 Publications

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with PF41. May form an antiparallel heterodimer with PF41.

Protein-protein interaction databases

STRINGi5833.PFF0615c-1.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 323
Turni33 – 364
Beta strandi50 – 556
Beta strandi62 – 665
Beta strandi70 – 723
Beta strandi75 – 784
Beta strandi119 – 1235
Beta strandi134 – 1407
Beta strandi161 – 1677
Beta strandi176 – 1805
Beta strandi187 – 1926
Helixi195 – 1984
Beta strandi209 – 2157
Beta strandi220 – 2245
Beta strandi230 – 2334
Beta strandi239 – 2457
Beta strandi247 – 2493
Beta strandi257 – 2626
Turni263 – 2664
Beta strandi267 – 2715
Beta strandi274 – 2763
Beta strandi280 – 2878
Beta strandi294 – 3007
Beta strandi302 – 3043

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YMOX-ray1.90A28-304[»]
ProteinModelPortaliC6KSX0.
SMRiC6KSX0. Positions 29-305.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 1721466-Cys 1
Add
BLAST
Domaini175 – 3051316-Cys 2
Add
BLAST

Sequence similaritiesi

Contains 2 6-Cys domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000281492.
OMAiESHYSAN.
PhylomeDBiC6KSX0.

Family and domain databases

InterProiIPR010884. Sexual_stage_antigen_S48/45.
[Graphical view]
PfamiPF07422. s48_45. 2 hits.
[Graphical view]
SMARTiSM00970. s48_45. 2 hits.
[Graphical view]
PROSITEiPS51701. 6_CYS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C6KSX0-1 [UniParc]FASTAAdd to Basket

« Hide

MIKLSKKYCL GISFVLYILL SVCEGHKNLT CDFNDVYKLE FHPNQQTSVT    50
KLCNLTPNVL EKVTIKCGSD KLNYNLYPPT CFEEVYASRN MMHLKKIKEF 100
VIGSSMFMRR SLTPNKINEV SFRIPPNMMP EKPIYCFCEN KKTITINGSN 150
GNPSSKKDII NRGIVEIIIP SLNEKVKGCD FTTSESTIFS KGYSINEISN 200
KSSNNQQDIV CTVKAHANDL IGFKCPSNYS VEPHDCFVSA FNLSGKNENL 250
ENKLKLTNII MDHYNNTFYS RLPSLISDNW KFFCVCSKDN EKKLVFTVEA 300
SISSSNTKLA SRDNTYQDYI SNSSFLTLSS YCAFITFIIT SFLSFIL 347
Length:347
Mass (Da):39,434
Last modified:September 1, 2009 - v1
Checksum:i0950614A8565DEA5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL844505 Genomic DNA. Translation: CAG25366.1.
RefSeqiXP_966114.1. XM_961021.1.

Genome annotation databases

EnsemblProtistsiPFF0615c:mRNA; PFF0615c:pep; PFF0615c.
GeneIDi3885775.
KEGGipfa:PFF0615c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL844505 Genomic DNA. Translation: CAG25366.1 .
RefSeqi XP_966114.1. XM_961021.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YMO X-ray 1.90 A 28-304 [» ]
ProteinModelPortali C6KSX0.
SMRi C6KSX0. Positions 29-305.
ModBasei Search...

Protein-protein interaction databases

STRINGi 5833.PFF0615c-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi PFF0615c:mRNA ; PFF0615c:pep ; PFF0615c .
GeneIDi 3885775.
KEGGi pfa:PFF0615c.

Organism-specific databases

EuPathDBi PlasmoDB:PF3D7_0612700.

Phylogenomic databases

HOGENOMi HOG000281492.
OMAi ESHYSAN.
PhylomeDBi C6KSX0.

Family and domain databases

InterProi IPR010884. Sexual_stage_antigen_S48/45.
[Graphical view ]
Pfami PF07422. s48_45. 2 hits.
[Graphical view ]
SMARTi SM00970. s48_45. 2 hits.
[Graphical view ]
PROSITEi PS51701. 6_CYS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  2. "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
    Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z.
    , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
    Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  3. "Predicted disulfide-bonded structures for three uniquely related proteins of Plasmodium falciparum, Pfs230, Pfs48/45 and Pf12."
    Carter R., Coulson A., Bhatti S., Taylor B.J., Elliott J.F.
    Mol. Biochem. Parasitol. 71:203-210(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum."
    Sanders P.R., Gilson P.R., Cantin G.T., Greenbaum D.C., Nebl T., Carucci D.J., McConville M.J., Schofield L., Hodder A.N., Yates J.R. III, Crabb B.S.
    J. Biol. Chem. 280:40169-40176(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  5. "Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum."
    Gilson P.R., Nebl T., Vukcevic D., Moritz R.L., Sargeant T., Speed T.P., Schofield L., Crabb B.S.
    Mol. Cell. Proteomics 5:1286-1299(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.
  6. Cited for: SUBCELLULAR LOCATION, CLEAVAGE, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, INTERACTION WITH PF41.
  7. "Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12) reveals a unique interdomain organization and the potential for an antiparallel arrangement with Pf41."
    Tonkin M.L., Arredondo S.A., Loveless B.C., Serpa J.J., Makepeace K.A., Sundar N., Petrotchenko E.V., Miller L.H., Grigg M.E., Boulanger M.J.
    J. Biol. Chem. 288:12805-12817(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 28-304, DISULFIDE BOND, SUBCELLULAR LOCATION, INTERACTION WITH PF41.

Entry informationi

Entry nameiPF12_PLAF7
AccessioniPrimary (citable) accession number: C6KSX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: September 1, 2009
Last modified: May 14, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Does not have erythrocyte-receptor-binding ability (1 Publication).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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