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C6HLY2

- LIPA_AJECH

UniProt

C6HLY2 - LIPA_AJECH

Protein

Lipoyl synthase, mitochondrial

Gene

HCDG_07601

Organism
Ajellomyces capsulatus (strain H143) (Darling's disease fungus) (Histoplasma capsulatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi141 – 1411Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi146 – 1461Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi152 – 1521Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi172 – 1721Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi176 – 1761Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi179 – 1791Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:HCDG_07601
    OrganismiAjellomyces capsulatus (strain H143) (Darling's disease fungus) (Histoplasma capsulatum)
    Taxonomic identifieri544712 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesAjellomycetaceaeAjellomyces
    ProteomesiUP000002624: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737MitochondrionUniRule annotationAdd
    BLAST
    Chaini38 – 430393Lipoyl synthase, mitochondrialPRO_0000398247Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliC6HLY2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C6HLY2-1 [UniParc]FASTAAdd to Basket

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    MATSAGKLRT LYSAHSSLSS LPPSARPTLQ LATLRSYATT TPHDSPIGNT    50
    SNTPPTVKRP ATAFKDKLNA GPAFSDFVSG KKDEPLDPAE AYALKTALVG 100
    PPGRKKEITR LPPWLKTPIP DSSNYKRIKN DLRGLNLHTV CEEARCPNIA 150
    DCWGGSSKSA ATATIMLMGD TCTRGCRFCS VKTSNKPPPL DPHEPDNTAE 200
    ALSRWGLGYV VLTTVDRDDL ADGGARHCAE TVMKIKQKAP NILVECLTGD 250
    YAGDLDMVAL VANSGLDVFA HNVETVEALT PFVRDRRASF QQSLRVLKAA 300
    KAAKPELITK TSLMLGLGET EAQLWDALRA LRAINVDVVT FGQYMRPTKR 350
    HMAVHEYVRP DVFDLWKERA LEMGFLYCAS GPLVRSSYKA GEAFIENVLK 400
    KRKGGNAGSV NEKVTTSENV KKLVAGEAMR 430
    Length:430
    Mass (Da):46,742
    Last modified:September 1, 2009 - v1
    Checksum:iED3348D459576A68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG692431 Genomic DNA. Translation: EER38732.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG692431 Genomic DNA. Translation: EER38732.1 .

    3D structure databases

    ProteinModelPortali C6HLY2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H143.

    Entry informationi

    Entry nameiLIPA_AJECH
    AccessioniPrimary (citable) accession number: C6HLY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3