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C6H178 (BGAL2_LACAI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase LacA

Short name=Beta-gal
EC=3.2.1.23
Gene names
Name:lacA
OrganismLactobacillus acidophilus
Taxonomic identifier1579 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length667 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes lactose, oNP-galactoside (oNPG), pNP-galactosidase (pNPG), pNP-mannoside, pNP-glucoside, pNP-fucoside, pNP-N-acetylglucosamide, but not pNP-arabinoside or 4-methylumbelliferyl-beta-galactopyranoside (MUG). Transgalactosylates lactose at 10 g/L, but not at 270 g/L. Ref.1

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Biophysicochemical properties

pH dependence:

Hydrolyzes lactose at pH 6.5, 6.8 and 7.2. Ref.1

Temperature dependence:

Hydrolyzes lactose between 30-45 degrees Celsius.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 667667Beta-galactosidase LacA
PRO_0000407691

Regions

Region355 – 3584Substrate binding

Sites

Active site1481Proton donor By similarity
Active site3071Nucleophile By similarity
Metal binding1131Zinc By similarity
Metal binding1531Zinc By similarity
Metal binding1551Zinc By similarity
Metal binding1581Zinc By similarity
Binding site1091Substrate By similarity
Binding site1471Substrate By similarity
Binding site3151Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
C6H178 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: C42D4FE9EE44BBD4

FASTA66776,705
        10         20         30         40         50         60 
MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP REGKYNFSKL 

        70         80         90        100        110        120 
DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ DRRHVFGQRH NFCPNSSNYQ 

       130        140        150        160        170        180 
RLAGELEKQL VERYKDNKHI VFWHINNEYG GNCYCENCQN AFKKWLKNKY KTVEGLNKAW 

       190        200        210        220        230        240 
NMNVWSHTIY DWDEIVVPNE LGDVWGIKGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII 

       250        260        270        280        290        300 
KKFDPETPVT TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH 

       310        320        330        340        350        360 
QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFRLKQA VGGSEKFHSA 

       370        380        390        400        410        420 
VIAHSQRTVT RVFKELADLG KKLKNAGPTI LGSKTKARFA IVFDWSNFWS YEYVDGITQD 

       430        440        450        460        470        480 
LNYVDSILDY YRQFYERNIP TDIIGVDDDF SNYDLVVAPV LYMVKHGLDK KINDYVENGG 

       490        500        510        520        530        540 
NFVTTYMSGM VNSSDNVYLG GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI 

       550        560        570        580        590        600 
CNLIHPNDAK ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG 

       610        620        630        640        650        660 
VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG EKAHKDMKGW 


DVQVLRN 

« Hide

References

[1]"Heterologous expression of glycoside hydrolase family 2 and 42 beta-galactosidases of lactic acid bacteria in Lactococcus lactis."
Schwab C., Soerensen K.I., Gaenzle M.G.
Syst. Appl. Microbiol. 33:300-307(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
Strain: FUA3191.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN424352 Genomic DNA. Translation: CAZ66938.1.

3D structure databases

ProteinModelPortalC6H178.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL2_LACAI
AccessionPrimary (citable) accession number: C6H178
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: September 1, 2009
Last modified: October 16, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries