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C6H178

- BGAL2_LACAI

UniProt

C6H178 - BGAL2_LACAI

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Protein

Beta-galactosidase LacA

Gene

lacA

Organism
Lactobacillus acidophilus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes lactose, oNP-galactoside (oNPG), pNP-galactosidase (pNPG), pNP-mannoside, pNP-glucoside, pNP-fucoside, pNP-N-acetylglucosamide, but not pNP-arabinoside or 4-methylumbelliferyl-beta-galactopyranoside (MUG). Transgalactosylates lactose at 10 g/L, but not at 270 g/L.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

pH dependencei

Hydrolyzes lactose at pH 6.5, 6.8 and 7.2.1 Publication

Temperature dependencei

Hydrolyzes lactose between 30-45 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091SubstrateBy similarity
Metal bindingi113 – 1131ZincBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Active sitei148 – 1481Proton donorBy similarity
Metal bindingi153 – 1531ZincBy similarity
Metal bindingi155 – 1551ZincBy similarity
Metal bindingi158 – 1581ZincBy similarity
Active sitei307 – 3071NucleophileBy similarity
Binding sitei315 – 3151SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase LacA (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:lacAImported
OrganismiLactobacillus acidophilus
Taxonomic identifieri1579 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Beta-galactosidase LacAPRO_0000407691Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC6H178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 3584Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

C6H178-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP
60 70 80 90 100
REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ
110 120 130 140 150
DRRHVFGQRH NFCPNSSNYQ RLAGELEKQL VERYKDNKHI VFWHINNEYG
160 170 180 190 200
GNCYCENCQN AFKKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE
210 220 230 240 250
LGDVWGIKGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKFDPETPVT
260 270 280 290 300
TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH
310 320 330 340 350
QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFRLKQA
360 370 380 390 400
VGGSEKFHSA VIAHSQRTVT RVFKELADLG KKLKNAGPTI LGSKTKARFA
410 420 430 440 450
IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF
460 470 480 490 500
SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG
510 520 530 540 550
GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK
560 570 580 590 600
ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG
610 620 630 640 650
VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG
660
EKAHKDMKGW DVQVLRN
Length:667
Mass (Da):76,705
Last modified:September 1, 2009 - v1
Checksum:iC42D4FE9EE44BBD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN424352 Genomic DNA. Translation: CAZ66938.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN424352 Genomic DNA. Translation: CAZ66938.1 .

3D structure databases

ProteinModelPortali C6H178.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Heterologous expression of glycoside hydrolase family 2 and 42 beta-galactosidases of lactic acid bacteria in Lactococcus lactis."
    Schwab C., Soerensen K.I., Gaenzle M.G.
    Syst. Appl. Microbiol. 33:300-307(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
    Strain: FUA3191Imported.

Entry informationi

Entry nameiBGAL2_LACAI
AccessioniPrimary (citable) accession number: C6H178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: September 1, 2009
Last modified: October 1, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3