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C6H178

- BGAL2_LACAI

UniProt

C6H178 - BGAL2_LACAI

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Protein

Beta-galactosidase LacA

Gene
lacA
Organism
Lactobacillus acidophilus
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes lactose, oNP-galactoside (oNPG), pNP-galactosidase (pNPG), pNP-mannoside, pNP-glucoside, pNP-fucoside, pNP-N-acetylglucosamide, but not pNP-arabinoside or 4-methylumbelliferyl-beta-galactopyranoside (MUG). Transgalactosylates lactose at 10 g/L, but not at 270 g/L.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

pH dependencei

Hydrolyzes lactose at pH 6.5, 6.8 and 7.2.1 Publication

Temperature dependencei

Hydrolyzes lactose between 30-45 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091Substrate By similarity
Metal bindingi113 – 1131Zinc By similarity
Binding sitei147 – 1471Substrate By similarity
Active sitei148 – 1481Proton donor By similarity
Metal bindingi153 – 1531Zinc By similarity
Metal bindingi155 – 1551Zinc By similarity
Metal bindingi158 – 1581Zinc By similarity
Active sitei307 – 3071Nucleophile By similarity
Binding sitei315 – 3151Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase LacA (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:lacA
OrganismiLactobacillus acidophilus
Taxonomic identifieri1579 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Beta-galactosidase LacAPRO_0000407691Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC6H178.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 3584Substrate binding

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

C6H178-1 [UniParc]FASTAAdd to Basket

« Hide

MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP    50
REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ 100
DRRHVFGQRH NFCPNSSNYQ RLAGELEKQL VERYKDNKHI VFWHINNEYG 150
GNCYCENCQN AFKKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE 200
LGDVWGIKGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKFDPETPVT 250
TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH 300
QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFRLKQA 350
VGGSEKFHSA VIAHSQRTVT RVFKELADLG KKLKNAGPTI LGSKTKARFA 400
IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF 450
SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG 500
GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK 550
ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG 600
VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG 650
EKAHKDMKGW DVQVLRN 667
Length:667
Mass (Da):76,705
Last modified:September 1, 2009 - v1
Checksum:iC42D4FE9EE44BBD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN424352 Genomic DNA. Translation: CAZ66938.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN424352 Genomic DNA. Translation: CAZ66938.1 .

3D structure databases

ProteinModelPortali C6H178.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Heterologous expression of glycoside hydrolase family 2 and 42 beta-galactosidases of lactic acid bacteria in Lactococcus lactis."
    Schwab C., Soerensen K.I., Gaenzle M.G.
    Syst. Appl. Microbiol. 33:300-307(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
    Strain: FUA3191.

Entry informationi

Entry nameiBGAL2_LACAI
AccessioniPrimary (citable) accession number: C6H178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: September 1, 2009
Last modified: June 11, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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