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C6H178

- BGAL2_LACAI

UniProt

C6H178 - BGAL2_LACAI

Protein

Beta-galactosidase LacA

Gene

lacA

Organism
Lactobacillus acidophilus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Hydrolyzes lactose, oNP-galactoside (oNPG), pNP-galactosidase (pNPG), pNP-mannoside, pNP-glucoside, pNP-fucoside, pNP-N-acetylglucosamide, but not pNP-arabinoside or 4-methylumbelliferyl-beta-galactopyranoside (MUG). Transgalactosylates lactose at 10 g/L, but not at 270 g/L.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    pH dependencei

    Hydrolyzes lactose at pH 6.5, 6.8 and 7.2.1 Publication

    Temperature dependencei

    Hydrolyzes lactose between 30-45 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateBy similarity
    Metal bindingi113 – 1131ZincBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Active sitei148 – 1481Proton donorBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Metal bindingi155 – 1551ZincBy similarity
    Metal bindingi158 – 1581ZincBy similarity
    Active sitei307 – 3071NucleophileBy similarity
    Binding sitei315 – 3151SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase LacA (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:lacAImported
    OrganismiLactobacillus acidophilus
    Taxonomic identifieri1579 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 667667Beta-galactosidase LacAPRO_0000407691Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliC6H178.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 3584Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C6H178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQLSRFLYG GDYNPDQWPE ETWSKDIHVF KKADINSATI NIFSWALLEP    50
    REGKYNFSKL DKVVQQLSDA NFDIVMGTAT AAMPAWMFKK YPDIARVDYQ 100
    DRRHVFGQRH NFCPNSSNYQ RLAGELEKQL VERYKDNKHI VFWHINNEYG 150
    GNCYCENCQN AFKKWLKNKY KTVEGLNKAW NMNVWSHTIY DWDEIVVPNE 200
    LGDVWGIKGS ETIVAGLSID YLRFQSESMQ NLFKMEKKII KKFDPETPVT 250
    TNFHGLPNKM VDYQKWAKGQ DIISYDSYPT YDAPAYKAAF LYDLMRSLKH 300
    QPFMLMESAP SQVNWQPYSP LKRPGQMEAT EFQAVAHGAD TVQFFRLKQA 350
    VGGSEKFHSA VIAHSQRTVT RVFKELADLG KKLKNAGPTI LGSKTKARFA 400
    IVFDWSNFWS YEYVDGITQD LNYVDSILDY YRQFYERNIP TDIIGVDDDF 450
    SNYDLVVAPV LYMVKHGLDK KINDYVENGG NFVTTYMSGM VNSSDNVYLG 500
    GYPGPLKEVT GIWVEESDAV VPGQKIKVLM NGKDYDTGLI CNLIHPNDAK 550
    ILATYASEFY AGTPAVTENQ YGKGRAWYIG TRLEHQGLTQ LFNHIIFETG 600
    VESLVCDSHK LEITKRVTED GKELYFVLNM SNEERTLPSK FTGYEDILTG 650
    EKAHKDMKGW DVQVLRN 667
    Length:667
    Mass (Da):76,705
    Last modified:September 1, 2009 - v1
    Checksum:iC42D4FE9EE44BBD4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN424352 Genomic DNA. Translation: CAZ66938.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN424352 Genomic DNA. Translation: CAZ66938.1 .

    3D structure databases

    ProteinModelPortali C6H178.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Heterologous expression of glycoside hydrolase family 2 and 42 beta-galactosidases of lactic acid bacteria in Lactococcus lactis."
      Schwab C., Soerensen K.I., Gaenzle M.G.
      Syst. Appl. Microbiol. 33:300-307(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
      Strain: FUA3191Imported.

    Entry informationi

    Entry nameiBGAL2_LACAI
    AccessioniPrimary (citable) accession number: C6H178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3