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C6EKG8 (C6EKG8_ECOBD) Unreviewed, UniProtKB/TrEMBL

Last modified November 16, 2011. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit beta HAMAP MF_01649
EC=1.14.12.19 HAMAP MF_01649
Alternative name(s):
Digoxigenin subunit beta HAMAP MF_01649
Gene names
Name:hcaF HAMAP MF_01649 EMBL ACT44251.1
Ordered Locus Names:B21_02395, ECBD_1145, ECD_02431
OrganismEscherichia coli (strain B / BL21-DE3) [Complete proteome] [HAMAP]
Taxonomic identifier469008 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length172 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP MF_01649

Catalytic activity

3-phenylpropanoic acid + NADH + O2 = cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD+. HAMAP MF_01649

Cinnamic acid + H+ + NADH + O2 = cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD+. HAMAP MF_01649

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01649

Subunit structure

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity. HAMAP MF_01649

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family. HAMAP MF_01649

Sequences

Sequence LengthMass (Da)Tools
C6EKG8 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: 58203B22B5D97554

FASTA17220,565
        10         20         30         40         50         60 
MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA QTRDRRKGVQ 

        70         80         90        100        110        120 
PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQVSETDIP NVFAVRVNYL 

       130        140        150        160        170 
LYRAQKERDE TFYVGTRFDK VRRLEDDNWR LLERDIVLDQ AVITSHNLSV LF

« Hide

References

[1]"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)."
Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F.
J. Mol. Biol. 394:644-652(2009) [PubMed: 19786035] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B / BL21-DE3 [Korea].
[2]"Sequencing and gene expression analysis of Escherichia coli BL21."
Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M.
Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B / BL21-DE3 [Austria].
[3]"Complete sequence of Escherichia coli BL21(DE3)."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B / BL21-DE3 [JGI].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001665 Genomic DNA. Translation: ACT28211.1.
CP001509 Genomic DNA. Translation: ACT44251.1.
AM946981 Genomic DNA. Translation: CAQ32912.1.

3D structure databases

ProteinModelPortalC6EKG8.
SMRC6EKG8. Positions 2-172.
ModBaseSearch...

Protein-protein interaction databases

STRINGC6EKG8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000157724; EBESCP00000149645; EBESCG00000159305.
EBESCT00000189081; EBESCP00000177838; EBESCG00000188491.
EBESCT00000194054; EBESCP00000180851; EBESCG00000192275.
GenomeReviewsGene locus B21_02395 in contig AM946981_GR.
Gene locus ECD_02431 in contig CP001509_GR.
Gene locus ECBD_1145 in contig CP001665_GR.
KEGGebd:ECBD_1145.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000011518.
OMAARRTICV.
ProtClustDBPRK10069.

Family and domain databases

HAMAPMF_01649. HcaF.
[Tree]
InterProIPR023712. HcaF.
IPR000391. Rng_hydr_dOase-bsu.
[Graphical view]
KOK05709.
PfamPF00866. Ring_hydroxyl_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC6EKG8_ECOBD
AccessionPrimary (citable) accession number: C6EKG8
Secondary accession number(s): C5W7P3
Entry history
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 16, 2011
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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